Presence of a classical RRM-fold palm domain in Thg1-type 3’- 5’nucleic acid polymerases and the origin of the GGDEF and CRISPR polymerase domains.
Vivek Anantharaman, Lakshminarayan M. Iyer, L. Aravind** Address for correspondence: L. Aravind (aravind@mail.nih.gov)
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
Abstract
Contents
Materials And Methods
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Multiple Alignment of Thg1 domain with the C terminal extension
Top
Family clusters of Thg1 domain containing proteins with domain architectures
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Prokaryotic Thg1 domain containing proteins with operons
Phylogenetic tree of Thg1 domain containing proteins
The tree was constructed with the Thg1 RRM-Palm domain alone (without the C terminal extension)
Archaea - Blue
Bacteria - Violet
Eukaryotes: Plants- Green; Animals- Red; Fungi- Grey; Apicomplexans- Brown; Amoebozoans/ bacterial transfers to eukaryotes- Ochre; Rest- Black
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Almost
all known nucleic acid polymerases catalyze 5’-3’
polymerization by mediating
the attack on an incoming nucleotide 5’ triphosphate by the
3’OH from the
growing polynucleotide chain in a template dependent or independent
manner. The
only known exception to this rule is the Thg1 RNA polymerase that
catalyzes
3’-5’ polymerization in vitro
and
also in vivo
as a part of the
maturation process of histidinyl tRNA. While the initiating reaction
catalyzed
by Thg1 has been compared to adenylation catalyzed by the aminoacyl
tRNA
synthetases, the evolutionary relationships of Thg1 and the actual
nature of
the polymerase reaction catalyzed by it remain unclear. Using sensitive
profile-profile comparison and structure prediction methods we show
that the
catalytic domain Thg1 contains a RRM (ferredoxin) fold palm domain,
just like
the viral RNA-dependent RNA polymerases, reverse transcriptases, family
A and B
DNA polymerases, adenylyl cyclases, diguanylate cyclases (GGDEF domain)
and the
predicted polymerase of the CRISPR system. We show just as in these
polymerases, Thg1 possesses an active site with three acidic residues
that
chelate Mg++
cations. Based on this we predict that Thg1
catalyzes
polymerization similarly to the 5’-3’ polymerases,
but uses
the incoming 3’ OH
to attack the 5’ triphosphate generated at the end of the
elongating
polynucleotide. In addition we identify a distinct set of residues
unique to
Thg1 that we predict as comprising a second active site, which
catalyzes the
initial adenylation reaction to prime 3’-5’
polymerization.
Based on contextual
information from conserved gene neighborhoods we show that Thg1 might
function in
conjunction with a polynucleotide kinase that generates an initial
5’ phosphate
substrate for it at the end of a RNA molecule. In addition to
histidinyl tRNA
maturation, Thg1 might have other RNA repair roles in representatives
from all
the three superkingdoms of life as well as certain large DNA viruses.
We also
present evidence that among the polymerase-like domains Thg1 is most
closely
related to the catalytic domains of the GGDEF and CRISPR polymerase
proteins.
Based on this relationship and the phyletic patterns of these enzymes
we infer
that the Thg1 protein is likely to represent an archaeo-eukaryotic
branch of
the same family of proteins that gave rise to the mobile CRISPR
polymerases and
in bacteria spawned the GGDEF domains. Thg1 is likely to be close to
the
ancestral version of this family of enzymes that might have played a
role in
RNA repair in the last universal common ancestor.
Contents
- Materials And Methods
- Multiple Alignment of Thg1 domain with the C terminal extension
- Family clusters of Thg1 domain containing proteins with domain architecture
- Operons of Thg1 containing prokaryotic proteins
- Phylogenetic tree of Thg1 domains
Materials And Methods
The
non-redundant (NR) database of protein sequences (
1.
Altschul
SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ:
Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs. Nucleic
Acids Res 1997,
25(17):3389-3402.
2.
Aravind L, Koonin EV:
Gleaning non-trivial structural,
functional
and evolutionary information about proteins by iterative database
searches.
J Mol Biol 1999,
287(5):1023-1040.
3.
Lassmann T, Sonnhammer EL:
Kalign--an accurate and fast multiple
sequence alignment algorithm. BMC
Bioinformatics 2005, 6:298.
4.
Lassmann T, Sonnhammer EL:
Kalign, Kalignvu and Mumsa: web
servers for
multiple sequence alignment. Nucleic
Acids Res 2006, 34(Web Server
issue):W596-599.
5.
Wootton JC: Non-globular
domains in protein sequences: automated
segmentation using
complexity measures. Comput
Chem 1994,
18(3):269-285.
6. Cuff JA, Barton GJ: Application of multiple sequence alignment profiles to improve protein secondary structure prediction. Proteins 2000, 40(3):502-511.
7. Eddy SR: Profile hidden Markov models. Bioinformatics 1998, 14:755-763.
8.
Soding
J: Protein homology
detection by HMM-HMM comparison. Bioinformatics
2005, 21:951-960.
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Multiple Alignment of Thg1 domain with the C terminal extension
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---------------------------E1-------------------------------H1------------------E2-------E3-------------------------------------H2----------------------------------------------------------------E4--------->>>C
terminal Extension FINAL ------HHHHHHH------------EEEEEE-----HH-HHHH----------HHHHHHHHHHHHHHHHHH-------EEEEE---EEEEEEE--------------------------HHHHHHHHHHHHHHHHHHHH------------------------------------------------------EEEE-----HHHHHHHHHHHH-HHH--HHHHHHHHHHHHHHH-----HHHHHHHHH---HHHHHHHHHHHH---------------EEEEE-------------------------------------------------------------------EEEE-----------------EEEEE------EEEEEE---------------H--HHHHHH------ THG1_Scer_6321461 MANSKFGYVRQFETHDV--ILP-QCYIVVRIDGKKFHE-FSKFYEFAKP---NDENALKLMNACAKNLVLK--YKNDIILAFGESDEYSFILKSS----------------TTLFNRRKDKLATLFGSFFTSNYVALWAKFFPEKPLNIKH------------------------------------LPYFDSRCVAYPN--LQTIKDYLSWRY-VDT--HINNLYNTTFWQLIIKCGLTPQESEKKLCGTFSNEKQEILFSECGINYNNEP-EMFKKGSLVTR---------------------------------------------------------------------------------------------------KGEILHI-----------NVIAQIDELFEG------- 6321461 Saccharomyces cerevisiae eukaryota>fungi>ascomycota ZYRO0A12144g_Zrou_254577809 MAKSRFEYVRDFEQRDV--LIP-ETYIVVRIDGKKFHE-FSNYYKFEKP---NDMRALKLMNACAKNVLLQ--YRSEVILAYGESDEYSFILRSD----------------TTLYNRRIDKISSLFVSLFTSQYVMLWSKFFPNDPLDVKH------------------------------------LPFFDSRCVSYPN--LKCIKDYLSWRF-VDT--HINNLYNTVFWQLVQKCGLTTREAENKLSGTVSSEKQEILFSECGINYNNEP-EMFKKGSLVTR---------------------------------------------------------------------------------------------------KGEILHI-----------DVIKKIDELFEG------- 254577809 Zygosaccharomyces rouxii CBS 732 eukaryota>fungi>ascomycota KLTH0G17270g_Lthe_255718999 MAKSRFEYVRQFESHET--LLP-ETYIVVRIDGKKFHE-FSKHYNFEKP---NDERALKLMNASAKNVVLK--YKNDVVLAFGESDEYSFILKKD----------------TSLFKRRRDKLSSLFVSLFTANYVALWSKFFPGTALDYKH------------------------------------LPFFDSRCVCYPN--LSTIKDYLCWRY-VDT--HINNLYNTVFWQLIIKCGLTPQESEQKLCGTLSSDKQEILFTDCGINYNDEP-NMFKKGSLVTN---------------------------------------------------------------------------------------------------KGEVCHI-----------DVVKQIDVLFEG------- 255718999 Lachancea thermotolerans CBS 6340 eukaryota>fungi>ascomycota AGOS_ABR031C_Agos_45185260 MAKSRFEYVREYEVHDT--LLP-ETYIVVRIDGKHFHE-FSQHYSFEKP---NDERALKLMNASAKNVVMA--YSGDIILAFGESDEYSFILRKD----------------STLFRRRRDKLSTLFVSLFTAQYVALWPKFFPEQPLSHKR------------------------------------LPFFDSRCVCYPN--TTVVKDYLCWRY-VDT--HINNLYNTVFWNLVLKCNLTPREAEQRLSGTLSSDKQEILFSECGVNYNNES-EMFKKGSLINR---------------------------------------------------------------------------------------------------KGEIMHI-----------DVVKQIDELFAG------- 45185260 Ashbya gossypii ATCC 10895 eukaryota>fungi>ascomycota EC1118_1G1_3257g_Scer_259146527 MANSKFGYVRQFETHDV--ILP-QCYIVARIDGKKFHE-FSKFYEFAKP---NDENALKLMNECAKNLVLK--YKNDIILAFGESDEYSFILKSS----------------TTLFNRRKDKLATLFGSFFTSNYVALWAKFFPEKPLNIKH------------------------------------LPYFDSRCVAYPN--LQTIKDYLSWRY-VDT--HINNLYNTTFWQLIIKCGLTPQESEKKLCGTFSNEKQEILFSECGINYNNEP-EMFKKGSLVTR---------------------------------------------------------------------------------------------------NGEILHI-----------NVIAQIDELFEG------- 259146527 Saccharomyces cerevisiae EC1118 eukaryota>fungi>ascomycota KLLA0B06237g_Klac_50303737 MAKSRFEYVRQFEVHDA--LLP-DTYIVVRVDGKKFHE-FSKYYNFAKP---NDERALKLMNAAAKNVFMQ--YKQEMICAYGESDEYSFILKRD----------------TKLFNRRRDKISTLFVSLFTANYVSLWNLFFPDVVLHHKH------------------------------------LPYFDSRCVCYPN--LTVVKDYLSWRF-VDT--HINNLYNTVFWYLIIKCGLTPQESEQKLCGTLSSDKQEILFSECGINYNNEP-EMYKKGSLVNS---------------------------------------------------------------------------------------------------KGEIVHI-----------DVVKQIDEIFNG------- 50303737 Kluyveromyces lactis NRRL Y-1140 eukaryota>fungi>ascomycota Kpol_526p19_Vpol_156843110 MANSRFEYVKQFETHDT--LLP-QTYIVVRIDGKKFHE-FSKFYDFAKP---NDERALKLMNACAKNVVLH--YKTDMIVAFGESDEYSFILKKD----------------TTLYRRRSEKLSTLFVSLFTSNYVALWPKFFPDTPLNHKY------------------------------------LPFFDSRCVVYPN--IQIIKDYLSWRF-VDT--HINNLYNTAFWQLIQICGLTAQEAEKKLMGTFSNDKQEILFTECGINYNNEP-EMYKKGSLITR---------------------------------------------------------------------------------------------------KGEILHI-----------DVIKQINELFVG------- 156843110 Vanderwaltozyma polyspora DSM 70294 eukaryota>fungi>ascomycota UM00383.1_Umay_71003728 MAGTKFAYVRNFELPDS--VIP-STYLVVRIDGKGFHK-FSAAHTFAKP---NDALALELMNEAARYVMYS--LKGHIALAFGESDEYSFLLRRS----------------TTLYNRRNSKITTHIVSLFTSAYVFSWSKFFPRNPL--LH------------------------------------PPSFDGRMVVYPN--EKVVRDYFSWRQ-ADT--HINNLYNTVFWALVLQAGLTEQEATKRLEGTVSADKHEILHSQFGINYDRLE-AMFRKGTTLVW-------------------------tcadssqeipshstaatgsqmeaekavtsdtveetqvltnlkdeklriknesrrvaakqakqekkdkks----MLSHHRHR-----------SNRD--LSWLTA------- 71003728 Ustilago maydis 521 eukaryota>fungi>basidiomycota CAGL0J00209g_Cgla_50290577 MAKSKYEYVKQFESHDT--LLP-QCYIVVRIDGKKFHE-FSKYYDFKKP---NDERALKLMNACAKNVVLQ--YRHEMILAYGESDEYSFVLKKD----------------TELYKRRRDKLSTLIVSLFTSNYVALWSKFFPGTNLHPKH------------------------------------LPFFDSRCVIYPN--LETIRDYVTWRY-VDT--HINNLYNTAFWQLIQKCGMNPQEAEKRLSGTVSSEKNEILFKECGINYNNEP-EMYKKGSLITN---------------------------------------------------------------------------------------------------KGEILHI-----------NVI---DSLDSLFEG---- 50290577 Candida glabrata CBS 138 eukaryota>fungi>ascomycota AgaP_AGAP011752_Agam_158300966 MALSRFEYVKQFEQEEK--LLP-NSWIVVRIDGKGFHR-FCNVHSFSKP---NDLDALQLMNLAGMTVLQE--F-NEIAIGYGQSDEYSFVFRRE----------------ASVYQRRRDKLVSYVASLFTSAYMFHWKRIFDGRSIAMRY------------------------------------PPSFDARAVLYPT--DENLRDYLSWRQ-ADV--HVNNLYNTTFWNLV-ASGLSNSDAEKRLQGTLASDKNEILFSQFGINYNNEP-IIYRKGTILLP-----------------------------------------------------------------------------------ksvvssegkkq----RLIVPIFE-----------DLIS--DAFWAKHPEILD- 158300966 Anopheles gambiae str. PEST eukaryota>metazoa>hexapoda CLUG_03111_Clus_260946739 MANSRFEYVKAFERENY--LLP-DTYIVIRVDGKGFHK-FSQEYSFAKP---NDVRALQVMNRAASAMLEQ--F-PDITMAYGDSDEYSFLLRRS----------------CALFERREMKLVSTFASFMSVHYVMQWNQEFPEKQIHSGR------------------------------------LPTFDARAVVYPN--AAVLRDYFSWRQ-VDC--HINNLYNTSFWGLVQKCGMSGQEAEQALSGTLAADKNEILFSRCGINYNNEP-EMFKKGSIIVR-------------------------------------------------------------------eyedwtpvdekgmtprqkqrvqkrrsk----AEIKVYHE-----------DIIK--DGFWDARPWLLE- 260946739 Clavispora lusitaniae ATCC 42720 eukaryota>fungi>ascomycota Phum_PHUM410370_Phum_242016870 MAKSKFEYVKEFETEDK--CLP-NTWIVVRVDGRGFSK-FSERYKFKKP---NDVRALSVMNAAAKAVMKE--F-PEIILAYGQSDEYSFVFKKD----------------STLYNRRIQKLTSVISSAFTAFYITLWPKYFGNESITT--------------------------------------PPIFDGRVVCYPS--ENNLRDYLSWRQ-TDT--HINNLYNTCFWNLVTNKKMSHKEAEEHLKGTVSSDKNELLFTEFGINYNNEP-EEFRKGTVIIR-------------------------------------------------------------------------------------------gs-----KKKLETHNC----------DIIG--NNFWDDHKELLN- 242016870 Pediculus humanus corporis eukaryota>metazoa>hexapoda zgc:101609_Drer_55925403 MAKSKFEYVRNFELDDT--CLR-NCYIVVRLDGRNLHK-FSDQHNFTKP---NDDRALGLMSRSACSVMEE--L-DDITIAYGQSDEFSFVFKRS----------------TNWFKRRASKLMTHVTSQFSSSFVFYWKEYFGEQPLLY--------------------------------------PPSFDGRVVLYPS--NRNVRDYLSWRQ-ADC--HINNLYNTAFWTLVQKGGLTTTQAEERLNGTLATDKNEILFSEFNINYNNES-SVYKKGTTLIW-------------------------------------------------------------------ekvnetttkqikrpneeetevtvtrt-----RKKVTSHSC----------DVIG--DQFWDEHPDILE- 55925403 Danio rerio eukaryota>metazoa>vertebrata>actinopterygii NEMVEDRAFT_v1g84802_Nvec_156403638 MAKSKYEYVRKFEQNDA--CLP-NCWIVVRVDGRNFHR-FSDSHGFKKP---NDPRGLGLMNKCAEAVMTE--F-GDIVICYGQSDEYSFVFRKN----------------TTQFSRRVSKLITNVVSLFAATYVFHWASFFTDQHLLY--------------------------------------PPMFDGRVVLYPS--DKNLRDYLSWRQ-ADC--HINNLYNTCFWCLVNQGGVTQTKAEERLCGTVSSDKNELLFSEFDVNYNNEP-ELYRKGSILIW--------------------------------------------------------------------------vqkneasteenttdkkkrir----RKVTTLHT-----------DIIG--DGFWDEHREILT- 156403638 Nematostella vectensis eukaryota>metazoa>cnidaria PF07_0095_Pfal_124511996 MANSKFSYVKLFEEERK--ILL-NCYFIVRIDGCDFKH-FVKAHNYNKP---NDIKGLNLMNECALDILKK--F-DDIDLCYGHSDEYSFLFNKS----------------TKLWNRRYDKILTNVVSYFTSCFLYKWKNYFQKEMLY---------------------------------------APSFDARIVVYPN--EKEIKDYFSWRQ-VDC--HINTQYNECFWNLIRQANYTNDEAHKFLLTTQTKDKNELLFSRFNINYNNLP-EIFRRGTIIIR-------------------------------------------nktfqkknealgtiynndtanniddnnnnnnnnnntfndnndienfeklkkecl-PKFIISHE-----------NLVS--EKFWDKYDYIFK- 124511996 Plasmodium falciparum 3D7 eukaryota>alveolata>apicomplexa TTHERM_00085230_Tthe_118358858 MACSQYEYVKKFETYQT--LLP-NTYIVVRIDGKGFTK-FTANHNFEKP---NDKRGLDLMNKAAESVMET--F-NEIMLAYGQSDEFSFVFKKD----------------AELYQRRTEKIVSCVVSCFTAAYAMHFSDYFNIKPSF---------------------------------------LPMFDARAVCYPD--FKNLRDYLNWRQ-VDC--HINNLYNTCFWTMVQKGNMTPQKAQEILKDTLSDRKNEILFNDYGINYAKLE-PQFRKGSTLIRvlvlkdkhankeaeqaesqqkrekceenqglnkegalqqqneegeeqkenaeellkeesdsvmtkgekkyiagqkkqkklnskq-ekiekskyr----SKIITLSD-----------DIIQ--NEFWDKYKDSLK- 118358858 Tetrahymena thermophila eukaryota>alveolata>ciliophora POSPLDRAFT_88866_Ppla_242214759 MAGSRYQYVKKFELPDP--ILP-GTFMVLRIDGHAFHR-LSEVHKFAKP---NDERALQLMDHAARDVMNE--Y-KDIVLAFGESDEYSFLFRKS----------------TALYNRRQAKIVTTLTSLFTSSYVFNWSRYLPDTPLEY--------------------------------------PPSFDGRIVVYPS--QKEIRDYFSWRQ-ADT--HINNLYNTIFWALVQQGGETTTQAHAILRGTVSGTKNEMLHSRFGINYNTIP-ARYRKGSVLVQervcslcswifelppassddlastagetp-----------gtpqqpeqapsg-----------------------------sskqkaskkahal----TRIELHHC-----------DIIG--DEFWDQRPYLLA- 242214759 Postia placenta Mad-698-R eukaryota>fungi>basidiomycota POSPLDRAFT_87675_Ppla_242205948 MAGSRYQYVKTFELPDP--ILP-GTFMVLRIDGHAFHR-LSEVHKFAKP---NDERALQLMDHAARDVMNE--Y-KDIVLAFGESDEYSFLFRKS----------------TALYNRRQAKIVTTLTSLFTSSYVFNWSRYLPDTPLEY--------------------------------------PPSFDGRIVVYPS--QKEIRDYFSWRQ-ADT--HINNLYNTIFWALVQQGGETTTQAHATLRGTVSGTKNEMLHSRFGINYNTIP-ARYRKGSVLVQersppassddlastagetqg---------------------tpqqpeqapsg-----------------------------ssrqkaskkahal----TKIELHHC-----------DIIG--DEFWDQRPYLLA- 242205948 Postia placenta Mad-698-R eukaryota>fungi>basidiomycota VDBG_07404_Valb_261358866 MANSKFEYVKAFEQPDT--LLQ-NTWVVVRIDGRGFTK-LCAKYAFEKP---NDKRALDLMNAAARAVMTD--L-PDITIAYGISDEYSFVFHKS----------------CTLFERRASKLVTTIVSTFTAHYIHSWSTYFPDAPLTLP-------------------------------------LPSFDGRAVCYPS--VQNLRDYMSWRQ-VDC--HINNLYNTTFWALIQLGGMDNRAAEELLAGTVSGDKNEILFSRFHINYNNEP-EMYKKGSVVFRdyelve----------------------------------pgthnvqadadaia---------------------epvsmtksqtekdkkrrnk----ARIVIEHL-----------DIIK--DDFWDRRPWLLS- 261358866 Verticillium albo-atrum VaMs.102 eukaryota>fungi>ascomycota NECHADRAFT_30739_Nhae_256734706 ---TRFEYVRTFETTDA--LLP-NTWIVVRVDGRGFTK-MCAKYGFEKP---NDRRALDLMNTAAKAVVTD--L-PEITIAYGVSDEYSFVFHKA----------------CTLFERRASKLVSTVVSTFTANYVFSWSTHFPDTPLSYP-------------------------------------LPTFDGRAVCYPS--VQNLRDYMSWRQ-VDC--HINNLYNTTFWSLIQLGGLDNKEAERTLAGTLAADKNEILFSRFSINYNNEP-EIFKKGSVIFR----------------------------------------d-----------------------------------pvqqsksqkekdkksrak----ARVVVEHL-----------DIIK--DDFWDRRPWLLS- 256734706 Nectria haematococca mpVI 77-13-4 eukaryota>fungi>ascomycota PODANSg09507_Pans_171695070 ---------------MT--VTN-MSLASLPNLSFCLTR-FSSKGFEKYP---NDRRALDLMNAAAQAVMYE--I-PEVTIAYGISDEYSFVFHKS----------------CTLFERRSSKLVTTIVSTFSSYYIHLWSTYFPDTPLSPP-------------------------------------LPSFDGRAVCYPS--VQNLRDYMSWRQ-VDC--HINNLYNTTFWALIQKGGMGNLEAEELLKGTYAADKNEILFSKFGINYNNEP-EIYKKGSVLFRglvd------------------------------------pathnaaaeadsla---------------------epvqqsknqtesdkkrrak----ARIVIEHL-----------DIIK--DEFWDRRPWLLS- 171695070 Podospora anserina S mat+ eukaryota>fungi>ascomycota PGUG_00408_Pgui_190344604 MANSRYEYVRQFERENF--LLP-DTYIIVRVDGKGFHK-FSDYYKFAKP---NDVGALEVMNEAALHVFKQ--I-SDIVMAYGDSDEYSFLLRKK----------------CSLYERREMKIVTLFAATMAAAYQHIWNTKFPEKPLQLER------------------------------------LPIFDARAVVYPS--MEHVSDYFRWRQ-VDC--HINNLYNTTFWALVSKGGMSPKEAENRLIGTVSSDKNEILFKEFGINYNNEP-EIFKKGTVIVR-------------------------------------------------------------------eyqesv--desqlsqrqkqryekkrgk----ASIMVHHI-----------DLIK--NDFWDSRPWLQN- 190344604 Pichia guilliermondii ATCC 6260 eukaryota>fungi>ascomycota PGUG_00408_Pgui_146422179 MANSRYEYVRQFERENF--LLP-DTYIIVRVDGKGFHK-FSDYYKFAKP---NDVGALEVMNEAALHVFKQ--I-SDIVMAYGDSDEYSFLLRKK----------------CSLYERREMKIVTLFAATMAAAYQHIWNTKFPEKPLQLER------------------------------------LPIFDARAVVYPS--MEHVSDYFRWRQ-VDC--HINNLYNTTFWALVLKGGMSPKEAENRLIGTVSSDKNEILFKEFGINYNNEP-EIFKKGTVIVR-------------------------------------------------------------------eyqesv--desqlsqrqkqryekkrgk----ASIMVHHI-----------DLIK--NDFWDSRPWLQN- 146422179 Pichia guilliermondii ATCC 6260 eukaryota>fungi>ascomycota LOC100264494_Vvin_225438521 INNIKPEYVKSFQFENK--LMP-LTWIVVRIDGCHFHR-FSEVHEFEKP---NDEQALNLMNSCAVAVLEQ--F-KDVIFSYGVSDEYSFVLKKD----------------SQFYQRRGSEIVTALVSYFTSIYVMKWKEFFPQKELKY--------------------------------------PPCFDGRAVLYPT--SEILRDYLTWRQ-VDC--HINNQYNTCFWMLV-KSGKSKSEAQAYLKGTQAREKNEVLLQQFGIDYNTLP-LMFRQGSSAFW--------------------------------------------------------------------------neeektgmye-ngasaeksg----KKVVVEQC-----------NIIE--QSFWEAHPSILN- 225438521 Vitis vinifera eukaryota>viridiplantae GSVIVT01022253001_Vvin_270232206 INNIKPEYVKSFQFENK--LMP-LTWIVVRIDGCHFHR-FSEVHEFEKP---NDEQALNLMNSCAVAVLEQ--F-KDVIFSYGVSDEYSFVLKKD----------------SQFYQRRGSEIVTALVSYFTSIYVMKWKEFFPQKELKY--------------------------------------PPCFDGRAVLYPT--SEILRDYLTWRQ-VDC--HINNQYNTCFWMLV-KSGKSKSEAQAYLKGTQAREKNEVLLQQFGIDYNTLP-LMFRQGSSAFW--------------------------------------------------------------------------neeektgmye-ngasaeksg----KKVVVEQC-----------NIIE--QSFWEAHPSILN- 270232206 Vitis vinifera eukaryota>viridiplantae AT2G31580_Atha_42569521 IGKIEPDFIRSFQFENK--LLP-LTWVVVRIDGCHFHR-FSDVHEFEKP---NDEQALKLMNSCAVAVLEE--F-EDIHFAYGVSDEYSFVLKKE----------------SELYKRQSSKIISAVASFFTSTYVLQWGEFFPHKELKY--------------------------------------PPSFDGRAVCYPT--YNILLDYLAWRQ-VDC--HINNQYNTCFWMLV-KSGKNKTQSQDYLKGTQTREKNELLSRQFGIEYNSLP-VIFRMGSSVFR-------------------------------------------------------------------------lkeaen------gvvsgkkle----GEVVVDHC-----------NIIE--RCFWEEHLHILS- 42569521 Arabidopsis thaliana eukaryota>viridiplantae yg1g_Omyk_259089291 MAKSKFEYVRNFETDDT--CLK-NCYIVVRLDGRNFHK-FAEQHNFLKP---NDDRALGLMTCSARSVMED--P-DDIIISYGQSDEFSFVFKRT----------------SNWFKRRASKLMTHVASQFSSSYVFYWKDYFGDQPLLY--------------------------------------PPGFDGRVVLYPS--NRNLRGYLSWRQ-ADC--HVNNLYNTVFWTLVQKGGLTTTQAEDRLKGTLASDKNEIMFFEFDINYNKEP-LVHRKGTTLIW-------------------------------------------------------------------ekleetvtksvklpneageevlvtrtr----RGVSAHHC-----------DVIG--NQFWEEHPNILE- 259089291 Oncorhynchus mykiss eukaryota>metazoa>vertebrata>actinopterygii thg1_Ssal_213514008 MAKSKFEYVRNFETDDT--CLK-NCYIVVRLDGRNFHK-FAEQHNFMKP---NDDRALGLMTCSARSVMED--L-DDIIISYGQSDEFSFVFKRT----------------SNWFKRRASKLMTHVASQFSSSYVFYWRDYFGDQPLLY--------------------------------------PPGFDGRVVLYPT--NRNLRDYLSWRQ-ADC--HVNNLYNTVFWTLVQKGGLTTTQAEDRLKGTLAADKNEIMFSEFDINYNKEP-LVHRKGTTLIW-------------------------------------------------------------------ekleetvtksvklpneageevlvtrtr----RRVGAHHC-----------DVIG--SQFWEEHPNILE- 213514008 Salmo salar eukaryota>metazoa>vertebrata>actinopterygii THG1_Eluc_225716354 MAKSKFEYVRNFEADDT--CLK-NCYIVVRLDGRNFHK-LSELHKFLKP---NDDRALGLMTRCARSVMED--M-DDIIISYGQSDEYSFIFKRS----------------SNWFKRRASKLMTHVVSQFSSSYVFYWKEYFGGQPLLY--------------------------------------PPGFDGRVVLYPS--NRNLRDYLSWRQ-ADC--HINNLYNTVFWTLVQRGGLTTTQAEDRLKGTLAADKNEIMFSEFDINYNNEP-LVHRKGTALIW-------------------------------------------------------------------ekmeetvtkrvklpsedekevpvtrfr----RRVSACHC-----------DIIG--DQFWEEHPDILE- 225716354 Esox lucius eukaryota>metazoa>vertebrata>actinopterygii BRAFLDRAFT_60263_Bflo_260828313 MAKSKFEYVRQFETQDP--CLP-NTWIVIRLDGRNFHR-FSTDHGFTKP---NDERALQLMNRAAETVMND--F-RDIVISYGQSDEFSFVLKKS----------------TMLYSRRASKLMTHIVSQFSSSYVFHWAQHFPDQPLQY--------------------------------------PPGFDGRVILYPS--NKNLRDYLSWRQ-ADC--HINNLYNTCFWALVQQGGMTNKQAEERIRHTFSADKNEILFSEFGINYNNEP-EMYKKGTVLVW------------------------------------------------------------------krveetvmkacrteddpverprevtklr----KKVVPLHT-----------DIIG--DVFWEEHPDILE- 260828313 Branchiostoma floridae eukaryota>metazoa LACBIDRAFT_252840_Lbic_170108182 MANSKYAYVRDFELPDP--LLP-GVFMVLRIDGHSFHR-FSEKHNFAKP---NDVRALQLMDHAAQDLMEE--Y-PDVVLGFGESDEFSFLLRKS----------------TSLYNRRHSKIVSTLTSLFTSSYVFHWSRYFPDTPLGY--------------------------------------PPSFDGRIVLYPG--TKEVKDYFAWRQ-ADT--HINNLYNTTFWALVQQGGQTTTEAHSTLRGTFAKDKHEILFSRFGINYNQLD-AQFRKGSVLVR------------------------------------------------------------------------------edleeekdgevsrrpk----SKVVMLHC-----------DIIK--DEFWEERSGLLV- 170108182 Laccaria bicolor S238N-H82 eukaryota>fungi>basidiomycota LOC100165160_Apis_193673956 MAKSKYEYVRTFEFEDR--CLQ-NCWIVVRLDGRSFHR-FTSSHKFEKP---NDKRALELMNRSAAAVMEE--F-RDVSLAYGQSDEYSFILRKN----------------TDLYNRRQSKIMSAINSIFSASYVYYWNTFFEDKKLLY--------------------------------------PPSFDARIVLYPT--DQNLRDYLSWRQ-ADT--HINNLYNTAFWGLISMKGLSNNEAEKVLSGTVSSEKNEILFKECGTNYNNEL-PIYRKGTVLVR----------------------------------------------------------------------------------klvnlppskskk----HVICPLHV-----------DIIG--DSFWEEYNEILN- 193673956 Acyrthosiphon pisum eukaryota>metazoa>hexapoda THG1L_Ggal_71895317 MAKSKFEYVRDFETDDT--CLP-NCWIVVRLDGRNFHR-FAEQHEFKKP---NDDRALHLMTKCAQTVMQE--L-EDIAIAYGQSDEYSFVFKKK----------------SKWFKRRASKFMTHVVSQFASSYVFYWKDYFKDQQLLY--------------------------------------PPGFDGRIVLYPS--NQNLKDYLSWRQ-ADC--HINNLYNTVFWMLVQRSGLTPVQAQDRLQGTLAGDKNEILFSEFNINYNNEP-LMYRKGTVLIW------------------------------------------------------------------qkvnevmtkkiklpkeseekevevtrtr----TKVVPLHC-----------DIIG--EQFWEEYPEILA- 71895317 Gallus gallus eukaryota>metazoa>vertebrata ACYPI006122_Apis_239788252 MAKSKYEYVRTFEFEDR--CLQ-NCWIVVRLDGRSFHR-FTSSHKFEKP---NDKRALELMNRSAAAVMEE--F-RDVSLAYGQSDEYSFILRKN----------------TDLYNRRQSKIMSAINSIFSASYVYYWNTFFEDKKLLY--------------------------------------PPSFDARIVLYPT--DQNLRDYLSWRQ-ADT--HINNLYNTAFWGLISMKGLSNNEAEKVLSGTVSSEKNEILFKECGTNYNNEL-PIYRKGTVLVR------------------------------------------------------------------klvnlpp----------------skskk----HVICPLHV-----------DIIG--DSFWEEYNEILN- 239788252 Acyrthosiphon pisum eukaryota>metazoa>hexapoda LOC100223262_Tgut_224067593 MAKSKFEYVRDFEADDT--VLP-NCWIVVRLDGRNFHR-FSEQHEFKKP---NDDRALQLMTKCAQTVMQE--L-EDIAIAYGQSDEYSFVFKKK----------------SRWFKRRASKFMTHVVSQFSSSYVFYWKDYFKDQQLLY--------------------------------------PPGFDGRIVLYPS--NQNLKDYLSWRQ-ADC--HINNLYNTVFWMLVQRGGLTPVQAQERLRGTLAGDKNEILFSEFNINYNNEP-LMYRKGTVLIW------------------------------------------------------------------qkinevitkkiklpkeeeekevevtrtk----TKVVPLHC-----------DIIG--DQFWEEYPEILA- 224067593 Taeniopygia guttata eukaryota>metazoa>vertebrata LOC100179812_Cint_198436739 MTHSQYDYVKSFEIPDP--CLP-NCWPVVRIDGKNFHK-FSTTHNFEKP---NDDRALKLMTAAATSVMDL--L-NDIVLAYGQSDEYSFVFKKK----------------TKAYNRRSSKLSSVVVSQFSTSYVYHWKKYFPDLELQY--------------------------------------PPAFDSRLVLYPS--DKNLRDYLSWRQ-VDC--HINNMYNYCFWKLV-QSGLTHVESQERLKGTLSGDKNELLFSQFGINYNNLP-QLHRKGTVIIR----------------------------------------dk--se-----------------------------------ngdctpgkngkdk----VAVVALNT-----------DIIG--KQFWEGRPHLLA- 198436739 Ciona intestinalis eukaryota>metazoa LOC663767_Tcas_91083329 MAQSKFEYVRKFETEEK--LLP-NCWIVVRIDGRAFHQ-FSTKHNFKKP---NDESALALMNKAASVVMNE--F-KDIVLAYGQSDEYSFVLRKD----------------TALYNRRGPKIMTYLSSLFTSSYVYHWNQFFKETKLKY--------------------------------------PPAFDARVVLYPS--DQNLRDYLSWRQ-ADC--HINNLYNTTFWALVLKGGLTNNEAEKRLCGTLSSDKNEILFSEFNTNYNNEP-EMFKKGTILIR----------------------------------------------------------------------------------krikspkhgkar----LVILPLHE-----------DLIQ--DKFWEKNSEILA- 91083329 Tribolium castaneum eukaryota>metazoa>hexapoda Pc16g14050_Pchr_255941868 MANSRFEYVKSFEQPDV--LLP-NTWIVVRIDGRGFHK-LSDHYAFAKP---NDRRALDLMNAAAVEVMKE--L-PDLCIAYGVSDEYSFVFHPN----------------CQLFERRNGKLVTTIVSTFTAHYIYKWSEYFPDRLLLPSH------------------------------------LPSFDGRAVIYPN--NRILRDYMSWRQ-VDC--HINNLYNTTFWTMVLQGGMSNTDAEQELKGTVSADKNEILFKRFGINYNNEL-EIYKKGTVLYR----------------------------------------qvy-eespv--------------------------seskqsrsqqdkirklrrk----AQVVIDHV-----------DIIK--DEFWEKRPWILS- 255941868 Penicillium chrysogenum Wisconsin 54-1255 eukaryota>fungi>ascomycota PKH_031670_Pkno_221052852 MANSKFAYVKQLEEERR--VLP-CCYFVVRIDGGNFKA-FTKTHGYTKP---NDIRGLHLMNACAKEVMEK--F-DEIDLAYGHSDEYSFLFRKK----------------TKVWNRRYDKILTNVVSCFSGSFPFLWKIFFPEQELLY--------------------------------------VPSFDGRIVLLPT--EREAKDYFRWRQ-VDC--HINTQYNECFWNLINKDGYSHQQAYNTLITTQKKEKNELLFSKFGINYNDLP-EIFRRGSILMRt---------------------------------------eqilkgghvaeqvditqmesdk-------------vappeeipsdaticqrdal----EKFTLSHE-----------NLVS--DIFWEKYHFLFA- 221052852 Plasmodium knowlesi strain H eukaryota>alveolata>apicomplexa TP01_0248_Tpar_71032267 MANSKYSYVREFEQDST--LLN-DCWIVVRVDGRAFSA-FSNRHEFRKP---NEPRALSVINAAAVNVMSE--F-DDIVLAYGHSDEYR--------------------------RKQ--KILSCVVSVFTSSYCYYWDTFFPNRPLLT--------------------------------------VPSFDGRVILYPT--YESVLDYFSWRH-VDC--HINNQYNTCFWCLILD-GKSNDEAYNWLKGTTKLEKNEYLFTSHKLNYNNLP-NIFKKGTTLVKsntkdvnsngencinynmfdqsdnmckr------------esgeilgeglvdkerkialei--------------nqselnelds-kvkeqasa----HNILVLHC-----------DIVK--DSFWELVS----- 71032267 Theileria parva strain Muguga eukaryota>alveolata>apicomplexa THG1_Lsal_225712230 KAHCKFEYVRQFETKET--LLP-NTWAVVRVDGRGFHA-FANKHEFTKP---NDVRALNLMNAAAKVVLGA--F-TDIVLGYGQSDEYSFVFRKS----------------ANLFSRRSAKIVTSVASLFASNYVYLWPEYFPDTKLKF--------------------------------------APSFDGRCVCYPS--DQNIRDYLSWRQ-ADC--HINNLYNTVFWALVQQGGMTNKEAQERLKGTLSADKNEILYSQFQINYNNES-QQFRKGSLLLK----------------------------------------kkvsvpvevhegnnassds----------------knedpstpskhqksgcrdr----VKIFDLNV-----------DIIG--DEFWENNEYIYS- 225712230 Lepeophtheirus salmonis eukaryota>metazoa>crustacea THG1_Lsal_225712866 KAHCKFEYVRQFETKET--LLP-NTWAVVRVDGRGFHA-FANKHEFTKP---NDVRALNLMNAAAKVVLGA--F-TDIVLGYGQSDEYSFVFRKS----------------ANLFSRRSAKIVTSVASLFASNYVYLWPEYFPDTKLKF--------------------------------------APSFDGRCVCYPS--DQNIRDYLSWRQ-ADC--HINNLYNTVFWALVQQGGMTNKEAQERLKGTLSADKNEILYSQFQINYNNES-QQFRKGSLLLK----------------------------------------kkvsvpvevhegnnass------------------dsknedpsk-hqksgcrdr----VKIFDLNV-----------DIIG--DEFWENNEYIYS- 225712866 Lepeophtheirus salmonis eukaryota>metazoa>crustacea MCYG_00432_Mcan_238837882 MANSKYEYVRNFEQSDV--LLP-NTWIVIRIDGRGFHK-LSDKYHFQKP---NDRRALDLMNSAAQAVMRD--I-PDLIMAYGVSDEFSFVFHPN----------------CQLFERRSSKLVSTIVSTFTAHYAFKWISFFPDTPLEPTF------------------------------------LPTFDGRAVQYPS--VKNLRDYMSWRQ-VDC--HINNLYNTTFWNMVQKGGMSNQDAEQELKGTVSSDKNEILFSRYGINYNNEL-EIFKKGSVLFR------------------dyeleq----------------vkrtpasiantnddrkw------------------envelsktqlekhqklrrk----ANVAIAHV-----------DIIK--DEFWEQRPWLLS- 238837882 Microsporum canis CBS 113480 eukaryota>fungi>ascomycota PAAG_05399_Pbra_226278784 SPNLLYEYVKAFEQDDN--LLP-NTWIVVRIDGRGFHR-FSGRYHFQKP---NDERALNLMNTAACAVMKD--L-PDLIIAYGVSDEYS-------------------------------KLVTTIVSTFTAHYIYNWSSFFPSAPLEPGF------------------------------------LPTFDGRAVQYPS--VRNLRDYMSWRQ-ADC--HINNLYNTTFWNMILRGGISNTEAEKELQYEIQPQTEE-----------------EKAGDG-------------------------------------------snevesetgeei-----------------------pptemtksqlarlrkiqkk----ATIVVKHM-----------DIIK--DDFWEQRPWILS- 226278784 Paracoccidioides brasiliensis Pb01 eukaryota>fungi>ascomycota PABG_05979_Pbra_225677608 ---VAYEYVKAFEQDDN--LLP-NTWIVVRIDGRGFHR-FSGRYHFQKP---NDERALNLMNTAACAVMKD--L-PDLIIAYGVSDEYS-------------------------------KLVTTIVSTFTAHYIYNWSSFFPSAPLEPGF------------------------------------LPTFDGRAVQYPS--VRNLRDYMSWRQ-ADC--HINNLYNTTFWNMILQGGISNTEAEKELQGTVSGEKNEILFSRFGINYNNEP-EMYRKGSVIFRdiahlfetqyetqpqteeekagdg----------------sneveaetgeev-----------------------pptemtksqlarlrkiqkk----ATIVVKHM-----------DIIK--DDFWEQRPWILS- 225677608 Paracoccidioides brasiliensis Pb03 eukaryota>fungi>ascomycota AFUB_014160_Afum_159131585 MANSKYEYVKSFEQPDV--LLP-NTWIVVRIDGRGFHK-LSDRYGFIKP---NDRRALDLMNAAAVEVMKE--L-PDLCIAYGVSDEYSFVFHPS----------------CQLFERRSAKLVTTIVSTFTAHYIYHWGTYFPSTPLHPPY------------------------------------LPSFDGRAVLYPT--TRILRDYMSWRQ-VDC--HINNLYNTTFWTMVQKGGMSNTDAERELQGTVSSDKNEILFKRFGINYNNED-EMFKKGSVVYRqvgqqgclydrrltwil-----qyql--------------edpkpesksrh---gdddeapm-------------deskisraqqdklrklrrk----AQVVVDHV-----------DIIK--DEFWERRPWILS- 159131585 Aspergillus fumigatus A1163 eukaryota>fungi>ascomycota ACLA_020800_Acla_121701067 MANSKYEYVKAFEQPDV--LLP-NTWIVVRIDGRGFHK-LSDRYEFIKP---NDRRALDLMNAAAVEVMKE--L-PDLCIAYGVSDEYSFVFHPS----------------CQLFERRSAKLVTTIVSTFTAHYIYQWGSYFPSMPLQAPH------------------------------------LPSFDGRAVLYPT--TRILRDYMSWRQ-VDC--HINNLYNTTFWTMVQKGGMSNTDAEKELQGTVSSDKNEILFKRFGINYNNED-EMYKKGSVLYR----------------------qyql--------------edpkptsdsksgtlgddgepalv------------qeasmsrsqqdkvrklrrk----AQVVVDHV-----------DIIK--DEFWERRPWILS- 121701067 Aspergillus clavatus NRRL 1 eukaryota>fungi>ascomycota AFUA_1G14630_Afum_70996154 MANSKYEYVKSFEQPDV--LLP-NTWIVVRIDGRGFHK-LSDRYGFIKP---NDRRALDLMNAAAVEVMKE--L-PDLCIAYGVSDEYRSLRFSP----------------QLSVVRATQRVSHPQPERASLPRLDLLTLQTLTLGPPY--------------------------------------LPSFDGRAVLYPT--TRILRDYMSWRQ-VDC--HINNLYNTTFWTMVQKGGMSNTDAERELHGTVSSDKNEILFKRFGINYNNED-EMFKKGSVVYRqvgqqgclydrrltwil-----qyql--------------edpkpesksrh---gdddeapm-------------deskisraqqdklrklrrk----AQVVVDHV-----------DIIK--DEFWERRPWILS- 70996154 Aspergillus fumigatus Af293 eukaryota>fungi>ascomycota NCU02105.1_Ncra_85111778 MANSKFEYVKQFEQPDS--LLP-NTWIVVRLDGRGFTK-FSTKYAFEKP---NDKRALDLMNAAARSVMSE--L-PDITIAYGVSDEYSFVFHKS----------------CTLFERRASKLVSTIVSTFTAYYIHHWPTYFVDGPPLSPP------------------------------------LPSFDGRAVCYPS--VQNLRDYMSWRQ-VDC--HINNLYNTTFWALINQGGMDGTAAELMLKGTFSADKNEILFKKFGINYNNEP-EMFKKGSVVFR---------------------nyelv--------------epgtkkvseee---aeemsssa-------------vpevksksqvekdkkvrtk----AKIVVEHL-----------DIIR--DEFWERRPWLLS- 85111778 Neurospora crassa OR74A eukaryota>fungi>ascomycota An01g12630_Anig_145230810 MANSKYEYVKSFEQPDA--LLP-NTWIVVRIDGRGFHK-LSDHYGFIKP---NDRRALDLMNAAAVGVMKD--L-PDLCIAYGISDEYSFAFHPN----------------CQLFERRSAKLVTTIVSTFTAHYIYLWGTYFPDTPLQPAA------------------------------------LPSFDGRAVMYPN--SRIFRDYMSWRQ-VDC--HINNLYNTTFWTMVLQGGMDRREAELELKGTLSSDKNEILFKRFGINYNNEE-EIYKKGSVIYR---q------e-----------------------------etspl------------------------------qedtpsktqqekirklrrk----VQVVVDHV-----------DIIK--DEFWERRPWILS- 145230810 Aspergillus niger CBS 513.88 eukaryota>fungi>ascomycota NFIA_010790_Nfis_119494994 MANSKYEYVKSFEQPDV--LLP-NTWIVVRIDGRGFHK-LSDRYGFIKP---NDRRALDLMNAAAVEVMKE--L-PDLCIAYGVSDEYSFVFHPS----------------CQLFERRSAKLVTTIVSTFTAHYIYQWGTYFPSTPLQPPY------------------------------------LPSFDGRAVLYPT--TRILRDYMSWRQ-VDC--HINNLYNTTFWTMVQKGGMSNTDAERELQGTVSSDKNEILFKRFGINYNNED-EMFKKGSVVYR----------------------qyql--------------edpkpesksrh---gdddealv-------------desktsrsqqdklrklrrk----AQVVVDHV-----------DIIK--DEFWERRPWILS- 119494994 Neosartorya fischeri NRRL 181 eukaryota>fungi>ascomycota AO090003000484_Aory_169770385 MANSKYEYVKAFEQPDV--LLP-NTWIVVRIDGRGFHK-LTDRYNFTKP---NDRRALDLMNAAAVEVMKD--L-PDLCIAYGVSDEYSFVFHPS----------------CQLFERRSAKLVTTIVSTFTAHYVYLWGTYFPDNPLQFPY------------------------------------LPSFDGRAVMYPA--TRNLRDYMSWRQ-VDC--HINNLYNTTFWTMVLQGGMSNTDAEQELKGTVSSDKNEILFKRFGINYNNEE-EIYKKGSVLYR----------------------qyqle-------------dikpksesksgvlaeeegnnv--------------qeakisrsqqdklrklrrk----AQVVVDHV-----------DIIK--DEFWERRPWILS- 169770385 Aspergillus oryzae RIB40 eukaryota>fungi>ascomycota cgd1_1990_Cpar_126643977 MACSKYEYIKTYEQPSR--IVK-NCWFVVRIDGHSFHE-FTKDHEFHKP---NDKRGLDLMSRCAENVMKN--L-GDIVISYGQSDEFSFVFRRK----------------TDLWSRKNDKILTHVVSLFTSSFIFFWDSFFPGKKLLY--------------------------------------PPTFDGRIIMYPT--DEDIRTYLSWRQ-ADC--HINNLYNTCFWSLVSINKLNEREATEKLKFTDSSYKNELLFKEFGINYNNIS-PQFRKGTTIYK---a-------------------------------------rpkekksrdeylllknndillfdkckeatietdhrdyteldkplnpiwkiddemviiSCIYKCHC-----------DIIQ--DKFWHENDHLLK- 126643977 Cryptosporidium parvum Iowa II eukaryota>alveolata>apicomplexa GSPATT00002474001_Ptet_145533455 MANSKFEYVKQFEQMQN--LLP-NTYIVVRIDGKGFHK-FTKCYDFEKP---NDEQGLKLMSFSACVVMET--F-PDIQIAYGQSDEFSFVLKKD----------------SELYSRRSDKIATCVCSTFTSVYTLNFEKFMKKPLQFPY-------------------------------------IPIFDARCVCYPD--LQNIRDYLSWRQ-ADC--HINNLYNTCFWGLVQKG-MNKQESEKTLAGTNAGDKNELLFSKLGKNYNNEL-EMFKKGTTIIRqptqervnlkveldvdskeqqklnkkqk------------kkqkykdlnqq-------------------------------elnqlpnqqked----LQVKEFYV-----------DIIK--DNFWIENKAYIL- 145533455 Paramecium tetraurelia strain d4-2 eukaryota>alveolata>ciliophora LOC100071194_Ecab_149726693 MAKSKFEYVRDFEADDT--CLA-HCWVVVRLDGRNFHR-FAEKHNFAKP---NDSRALHLMTKCAQTVMQE--L-EDIVIAYGQSDEYSFVFKRK----------------TNWFKRRASKFMTHVASQFASSYVFYWRDYFEEQPLLY--------------------------------------PPGFDGRVVVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALVQQSGLTPAQAQERLQGTLAADKNEILFSEFNINYNDEP-LMYRKGTVLIW-----------------------------------------------------------------qkvgevtt-kevklpaemegkkmavirtr----TKPVPLYC-----------DIIG--DAFWKEHPEILH- 149726693 Equus caballus eukaryota>metazoa>vertebrata Thg1l_Rnor_62078661 MAKSKFEYVRDFEVDDT--CLP-HCWVVVRLDGRNFHR-FAEKHNFAKP---NDSRALHLMTKCAQTVMQE--L-EDIVIAYGQSDEYSFVFRKR----------------SNWFKRRASKFMTLVASQFASSYVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVVLYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALIQQSGLTPVQAQQRLKGTLTADKNEILFSEFHINYNNEP-HMYRKGTVLVW-----------------------------------------------------------------qkvnevrt-qeirlpaemegekmavtrtr----TKLVALNC-----------DLIG--DAFWKEHPEILE- 62078661 Rattus norvegicus eukaryota>metazoa>vertebrata Thg1l_Mmus_62530979 MAKSKFEYVRNFEVQDT--CLP-HCWVVVRLDGRNFHR-FAEEHNFAKP---NDSRALHLMTKCAQTVMEE--L-EDIVIAYGQSDEYSFVFRKK----------------SNWFKRRASKFMTLVASQFASSYVFYWRDYFEDQPLRY--------------------------------------PPGFDGRVVLYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWVLIQQSGLTPVQAQQRLKGTLTADKNEILFSEFHINYNNEP-HMYRKGTVLVW-----------------------------------------------------------------kve-evrtqevrlpa-emegekkavartr----TRVVALNC-----------DLIG--DAFWKEHPEILA- 62530979 Mus musculus eukaryota>metazoa>vertebrata Thg1l_Mmus_124377988 MAKSKFEYVRNFEVQDT--CLP-HCWVVVRLDGRNFHR-FAEEHNFAKP---NDSRALHLMTKCAQTVMEE--L-EDIVIAYGQSDEYSFVFRKK----------------SNWFKRRASKFMTLVASQFASSYVFYWRDYFEDQPLRY--------------------------------------PPGFDGRVVLYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALIQQSGLTPVQAQQRLKGTLTADKNEILFSEFHINYNNEP-HMYRKGTVLVW-----------------------------------------------------------------qkveevrt-qevrlpaemegekkavartr----TRVVALNC-----------DLIG--DAFWKEHPEILA- 124377988 Mus musculus eukaryota>metazoa>vertebrata THG1L_Btau_122692489 MAKSKFEYVRDFEADDT--CLP-HCWVVVRLDGRNFHR-FAEKHSFIKP---NDSRALHLMTKCAQTVMNE--L-EDIVIAYGQSDEYSFVFKRK----------------SNWFKRRASKFMTHVVSQFASSYVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVIVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALVQQSGLTPLQAQERLQGTLAADKNEILFSEFNINYNNEP-LMYRKGTVLIW-----------------------------------------------------------------qkveeitt-kevklpaemegkkmavtrtr----TMVVPLHC-----------NIIG--DAFWKEHPEILD- 122692489 Bos taurus eukaryota>metazoa>vertebrata IscW_ISCW001226_Isca_241096199 MAKSKFEYVRQFEQ--DDRCLP-NCWLVVRVDGKAFHR-FSDAHNFEKP---NDKRALDLMSRCAERVMDE--F-KDICLSYGQSDEYSFVFRK----------------DSLVYNRRASKLMTSVSSLFTSAYVFHWPEYFPDSM-RY--------------------------------------PPSFDGRVVLYPS--DKNLVDYLSWRQ-ADC--HINNLYNTVFWSLVQSGGLSPKQAEERLRGTLSSDKNEILFQEFGINYNNLS-PLYRKGTVIVR-----------------------------------------------------------------------epvpdetapaeggkkpgkqkdth----FFMKNSTN-----------KIYS-----TLEMCYIIDL 241096199 Ixodes scapularis eukaryota>metazoa PANDA_000301_Amel_281345114 MAKSKFEYVRNFEADDT--CLA-HCWVVVRLDGRNFHR-FAEKHNFAKP---NDSRALHLMTKCAQTVMKE--L-EDIVIAYGQSDEYSFVFKRK----------------SNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVIVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALVQQSGLTPVQAQERLQGTLAADKNEILFSEFNINYNNEP-LMYRKGTVLIW-----------------------------------------------------------------kvg-evttkevklpa-emegkkmemtrtr----TKPVPLHC-----------DIIG--DAFWKEHPEILD- 281345114 Ailuropoda melanoleuca eukaryota>metazoa>vertebrata THG1L_Hsap_89242148 MAKSKFEYVRDFEADDT--CLA-HCWVVVRLDGRNFHR-FAEKHNFAKP---NDSRALQLMTKCAQTVMEE--L-EDIVIAYGQSDEYSFVFKRK----------------TNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVVVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALIQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNEL-PMYRKGTVLIW-----------------------------------------------------------------qkvdevmt-keiklptemegkkmavtrtr----TKPVPLHC-----------DIIG--DAFWKEHPEILD- 89242148 Homo sapiens eukaryota>metazoa>vertebrata Dyak\GE24732_Dyak_195474109 MACSRFEYVKSFEQDDS--ILP-NVWIVIRIDGKKFHK-FSKTHDFEKP---NDENALNVMNSAATAVMQE--F-RDIVVAYGQSDEYSFVFRKE----------------TAAFKRRSAKLLTYVTSLFSSSYVMQWSKWMSQPLAY---------------------------------------APCFDGRVVLYPS--EQNLKDYLSWRQ-ADV--HVNNLYNTAFWKLVLEKDLTNQQAEAKLRGTFSADKNELLFQEFGINYNNLP-AMYRKGTILLR------------------------------------------------------------------------------------krvilgdksr----QAVVPLHE-----------DLIS--SQFWKEHNEILG- 195474109 Drosophila yakuba eukaryota>metazoa>hexapoda LOC479315_Clup_57085243 MAKSKFEYVRNFEADDT--CLA-HCWVVVRLDGRNFHR-FAEKHNFAKP---NDSRALHLMTKCAQTVMKE--L-EDIVIAYGQSDEYSFVFKRK----------------SNWFKRRASKFMTHVASQFASSFVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVIVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALVQQSGLTPAQAQERLQGTLAADKNEILFSEFNINYNNEP-LMYRKGTVLIW-----------------------------------------------------------------qqkvgevttkevklpeemqgkkmavtrtr----TKPVPLYC-----------DIIG--DAFWKEHPEILD- 57085243 Canis lupus familiaris eukaryota>metazoa>vertebrata CG4103_Dmel_19921364 MACSRFEYVKSFEQDDS--ILP-NVWIVIRIDGKKFHK-FSKTHDFEKP---NDENALNVMNAAATAVMQE--F-RDIVLAYGQSDEYSFVFRKE----------------TAAFKRRSAKLLTYVTSLFSSSYVMQWSKWMNLPLAY---------------------------------------APCFDGRVVLYPS--EQNLKDYLSWRQ-ADV--HVNNLYNTAFWKLVLEKGLTNQQAEAKLRGTFSADKNELLFQEFGINYNNLP-AMYRKGTILLR------------------------------------------------------------------------------------krvilgeksr----QAVVPLHE-----------DLIS--SQFWKEHTEILG- 19921364 Drosophila melanogaster eukaryota>metazoa>hexapoda _Hsap_62897799 MAKSKFEYVRDFEADDT--CLA-HCWVVVRQDGRNFHR-FAEKHNFAKP---NDSRALQLMTKCAQTVMEE--L-EDIVIAYGQSDEYSFVFKRK----------------TNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVVVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALIQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNEP-PMYRKGTVLIW-----------------------------------------------------------------qqkvdevmtkeiklptemegkkmavtrtr----TKPVPLHC-----------DIIG--DAFWKEHPEILD- 62897799 Homo sapiens eukaryota>metazoa>vertebrata LOC462226_Ptro_114603135 MAKSKFEYVRDFEADDT--CLA-HCWVVVRLDGRNFHR-FAEKHNFAKP---NDSRALQLMTKCAQTVMEE--L-EDIVIAYGQSDEYSFVFKRK----------------TNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVVVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALIQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNEP-LMYRKGTVLIW-----------------------------------------------------------------kvd-evmtkeiklpt-emegkkmavtrtr----TKPVPLHC-----------DIIG--DAFWKEHPEILD- 114603135 Pan troglodytes eukaryota>metazoa>vertebrata Dsim\GD21976_Dsim_195579310 MACSRFEYVKSFEQDDS--ILP-NVWIVIRIDGKKFHK-FSKTHDFEKP---NDENALNVMNAAATAVMQE--F-RDIVLAYGQSDEYSFVFRKE----------------TAAFKRRSAKLLTYVTSLFSSSYVMQWSKWMNLPLAY---------------------------------------APCFDGRVVLYPS--EQNLKDYLSWRQ-ADV--HVNNLYNTAFWKLVLEKGLTNQQAEAKLRGTFSADKNELLFQEFGINYNNLP-AMYRKGTILLR-----------------------------------------------------------------krvil-------------------geksr----QAVVPLHE-----------DLIS--SQFWKEHTEILG- 195579310 Drosophila simulans eukaryota>metazoa>hexapoda LOC100075219_Oana_149412544 MAKSKFEYVRAFEADDT--CLP-HCWVVIRLDGRNFHR-FADQHNFAKP---NDDRALHLMNKCAQVVMQE--L-EDIVIAYGQSDEYSFVFKKM----------------SNWFKRRASKFMTHVASQFASSYVFYWKDYFKDQPLLY--------------------------------------PPGFDGRVVLYPS--NQNLKDYLSWRQ-ADC--HINNLYNTVFWMLVQRSGLTPVQAQERLKGTLAADKNEILFSEFNINYNNES-PMYRKGTVLIW-----------------------------------------------------------------qkvsevtt-keskqpedkeeqkvevtrtr----TKVVPLHC-----------DIIG--DAFWKEHQEILA- 149412544 Ornithorhynchus anatinus eukaryota>metazoa>vertebrata _Hsap_7020726 MAKSKFEYVRDFEADDT--CLA-HCWVVVRLDGRNFHR-FAEKHNFAKP---NDSRALQLMTKCAQTVMEE--L-EDIVIAYGQSDEYSFVFKRK----------------TNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVVVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALIQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNEP-PMYRKGTVLIW-----------------------------------------------------------------qkvdevmt-keiklptemegkkmavtrtr----TKPVPLHC-----------DIIG--DAFWKEHPEILD- 7020726 Homo sapiens eukaryota>metazoa>vertebrata LOC715853_Mmul_109079563 MAKSKFEYVRDFEADDT--CLA-HCWVVVRLDGRNFHR-FAEKHNFAKP---NDSRALHLMTKCAQTVMEE--L-EDIVIAYGQSDEYSFVFKRK----------------TNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVVVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALIQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNEP-LMYRKGTVLIW-----------------------------------------------------------------qkvdevmt-keiklptemegkkmavtrtr----TKPVPLHC-----------DIIG--DAFWKEHPDILD- 109079563 Macaca mulatta eukaryota>metazoa>vertebrata LOC100025355_Mdom_126290678 MAKSKFEYVRDFEADDT--CLA-HCWVVVRLDGRNFHR-FAEKHNFTKP---NDSRALDLMTRCAQTVMTE--L-EDIVMAYGQSDEYSFVFKRK----------------SNWFRRRASKFMTNAASQFASSYVFYWKDYFKDQDLLY--------------------------------------PPAFDGRVVVYPS--NQTIKDYLSWRQ-ADC--HVNNLYNTVFWMLIQRSKLTPAQAQERLRGTLAADKNEILFSEYNINYNNEP-PMFRKGTVLIW-----------------------------------------------------------------qkkikeikskeikppgetegkqvevtrir----TKPVALHC-----------DIIG--DTFWKEHPEILE- 126290678 Monodelphis domestica eukaryota>metazoa>vertebrata LOC100353509_Ocun_291387726 MAKSKFEYVRDFEADDT--CLA-HCWVVVRLDGRNFHR-FSEKHNFAKP---NDSRALHLMTKCAQTVMEE--L-EDIVIAYGQSDEYSFVFKRK----------------SNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLY--------------------------------------PPGFDGRVVVYPS--NQTLKDYLSWRQ-ADC--HINNLYNTVFWALVQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNEP-LMYRKGTVLIW-----------------------------------------------------------------qkvgevtt-kevklpaemegkkmavtrtr----SKPVALHC-----------DLIG--DAFWKEHPEILS- 291387726 Oryctolagus cuniculus eukaryota>metazoa>vertebrata Dsec\GM14588_Dsec_195338513 MACSRFEYVKSFEQDDS--ILP-NVWIVIRIDGKKFHK-FSKTHDFEKP---NDENALNVMNAAATAVMQE--F-RDIVLAYGQSDEYSFVFRKE----------------TATFKRRSAKLLTYVTSLFSSSYVMQWSKWMNLPLAY---------------------------------------APCFDGRVVLYPS--EQNLKDYLSWRQ-ADV--HVNNLYNTAFWKLVLEKGLTNQEAEAKLRGTFSADKNELLFQEFGINYNNLP-AMYRKGTILLR------------------------------------------------------------------------------------krvilgeksr----QAVVPLHE-----------DLIS--SQFWKEHTEILG- 195338513 Drosophila sechellia eukaryota>metazoa>hexapoda THG1_Ccle_225719698 MAHSKFEYVRSFESKDS--LLP-NTWLVVRIDGRGFHA-FSNEHDFEKP---NDVRALNLMNAAAKVVIEA--F-TDTVLAYGQSDEYSFVFRRN----------------TNLYSRRSAKIATNVTSLFAANYVYLWPQFFPDKPLKV--------------------------------------APSFDGRCVCYPT--DANLRDYLSWRQ-ADC--HINNLYNTVFWALVLKGGLTNREAQERLKGTLSGDKNEILFSQFQINYNQEA-QQFRKGSTLLK----------------------------------------kapvpveipqdkked-------------------------spkrqkensgfrdr----VKIFDLNI-----------DLIG--DDFWKENAHIYS- 225719698 Caligus clemensi eukaryota>metazoa>crustacea LOC100117826_Nvit_156547518 MAKSKYDYVKDFEHEDN--CLP-NCWIVVRIDGRNFSK-FADSHQFVKP---NDLAALELMNRAAMTVMED--F-REIVIAYGQSDEYSFVFRKD----------------TQLFKRRASKLMSNVNSLFASAYVYNWPRFFKNRELHY--------------------------------------PPSFDARVVLYPT--DKNLRDYLAWRQ-ADV--HINNLYNTCFWSLVLKKHLTPQQAEERLSGTLSSHKNELLYQEFGINYNNEP-AVYRKGTTLLR-----------------------------------------------------------------------------------klvahgngrlk----PTVVPLVD-----------DIIG--DRFWKENPEVIG- 156547518 Nasonia vitripennis eukaryota>metazoa>hexapoda LOC692885_Bmor_114051932 MAKSSFEYVKKFEHDDS--LLP-NTWIVIRLDGKCFHK-FADDHNFTKP---NDLRALKLMNYAAYTVLKE--Y-SDILLSFGQSDEYSFVLRKD----------------SCLYKRRSAKLLTTINSKFSSSYVFYWNKFFEHLPLKY--------------------------------------PPCFDGRIVLYPC--DENLIDYMKWRQ-ADV--HINNLYNTTFWTLILKGQLTPVQAEKRLSGTVSADKNEILFQEFNMNYNNEP-EIFKRGTILLR-----------------------------------------------------------------------------------ksilh-nkvnk----SIIVDVHD-----------DMLK--DKFWKENIYILS- 114051932 Bombyx mori eukaryota>metazoa>hexapoda SJAG_04233_Sjap_213409163 MANSRFEYVKGFEKADN--LLP-ETYIVIRIDGKGFHK-FTEKHEFAKP---NDSRCLELMNKAAEIVVSE--F-TDIVLAYGDSDEYSFVWCKG----------------TQLYERRESKLVSHVCSLFTSAFVFNWSKFFDIPLRS---------------------------------------LPSFDGRAVLYPS--FSSLRDYLSWRQ-ADC--HINNLYNTTFWALRLQGKMSNREAEERLKGTVSADKHEILFSQFGINYNNEP-EMYKKGTIFTRpadgddmls-------------------------------------------------------------------------kgtnlskkqkkk----LVIEKLHV-----------DLIA--DSFWKERPYLET- 213409163 Schizosaccharomyces japonicus yFS275 eukaryota>fungi>ascomycota thg1_Ddis_66802352 MANSKYEYVKSFEQPDI--LLQ-NVWIVVRIDGRSFHK-FTTKHDYAKP---NDDRGLSLMNRAALEVCKE--F-PDIVIAFGESDEYSFVLKKS----------------CNLFERRSSKISSSIVSYFTSQFVYRWKEYFGEHELKY--------------------------------------PPTFDSRCVLYPT--DENIKDYLSWRQ-ADT--HINNLYNTCYWALVLKAGKTPIEAENELRGTFSDGKNEMLFSRFNINYNNLP-AEYRKGSVIFK------------------------------------------------------------------------------kpvqetnketgltksk----KRLVIEHV-----------DIIS--EKFWKEYPDILA- 66802352 Dictyostelium discoideum AX4 eukaryota>amoebozoa>mycetozoa>dictyosteliida LOC100205752_Hmag_221135876 MAKSKYEYVKKFEQNET--CLL-NCWIVVRIDGRGFHK-FTHDHLYEKP---NDIRGLSLMNFCAKEVMKQ--F-QDIVISYGQSDEYSFVFSKN----------------TSQFKRRSCKLMSNIVSLFSSSFVFYWKTFFLNDLIY---------------------------------------PPQFDGRIILYPS--LENIRDYLSWRQ-ADC--HINNLYNTCFWSLVNKGGLSTLDAELKLKGTLAKDKNELLFSEFDVNYNDIS-PIFRKGNIVIR------------------------------------------------------------------------qkvaeeimkekngesvkvikek----NDTVILHD-----------DIIG--ENFWKKFPEILT- 221135876 Hydra magnipapillata eukaryota>metazoa>cnidaria _Gmor_289742577 MACSRYEYVKSYEVQDV--LLP-NVWIIIRIDGKGFHK-FCKAHDFEKP---NDERALNLMTSAAQTVMEK--F-SDIILSYGQSDEYSFVFRKE----------------TNVYNRRSSKLLSYVTSLFTSCYVMYWPQWMGNQKLTY--------------------------------------PPSFDGRVVLYPS--DQNLRDYLSWRQ-ADV--HINNLYNTAFWNLVQKKGLTNREAEAKLRGTYAAYKNELLFSEFGINYNNLP-GMFRKGTILLR----------------------------------------------------------------------------------kqvnrtkheisk----QLIVPFHE-----------DMIS--DIFWKKHSELLA- 289742577 Glossina morsitans morsitans eukaryota>metazoa>hexapoda Dere\GG24229_Dere_194857325 MACSRFEYVKSFELDDS--ILP-NVWIVIRIDGKKFHK-FSKAHDFEKP---NDENALNVMNAAATAVMQE--F-RDIVLAYGQSDEYSFVFRKE----------------TAAFKRRAAKLLTYVTSLFSSSYVMQWPKWMQLPLAY---------------------------------------APCFDGRVVLYPS--EQNLKDYLSWRQ-ADV--HVNNLYNTAFWKLVLEKGLTNQQAEAKLRGTFSADKNELLFQEFGINYNNLP-AMYRKGTILLR------------------------------------------------------------------------------------krvilgeksr----QAVVPLHE-----------DLIS--SQFWKKHTEILG- 194857325 Drosophila erecta eukaryota>metazoa>hexapoda TVAG_470220_Tvag_123437995 MACSKWEYVKDFELDDR--LLP-STYIVVRVDGRGFTE-FCINHNLEKP---LDDRLIRLMSNCAQKVMLK--F-DEMVLAFGESDEFSFIFKKS----------------AKVFNRRRDKINSTVASLFSSIFVKDWSNFFPNLPLQD--------------------------------------PPSFDSRIVLYPS--LDVVKDYLCWRQ-ADT--HINCLYNYTLNVLL-RAGENPTDATEKLRGTFSNDKNEILFKH-GINYKLLP-AAHRKGTVWIH--------------------------------------------------------------------------------------------ak-----KLFETND-----------DLIQ--DAFWKKYSKLFE- 123437995 Trichomonas vaginalis G3 eukaryota>parabasalia LOC100374565_Skow_291224944 MANSKYEYVRHFETQDR--CLP-NCWIVVRLDGKNFHK-FSDSHEFSKP---NDEAALNLMNCCAVYVMNE--F-QDITVAYGESDEYSFVFRKG----------------TTQFSRRASKLMTNVVSLFAASYVFNWSKFFPNKQLMY--------------------------------------PPAFDCRVVVYPS--DENLRDYLSWRQ-ADC--HINNLYNTCFWKLVLQGGYSTKEAEQKLKGTYSSDKNELLFSQFDINYNELP-QLFRKGTVLFWqkveeklm------------------------------------------------------------------khfkskdserletkevtrvr----NVVVTQHI-----------DIIG--DEFWKLHPEILG- 291224944 Saccoglossus kowalevskii eukaryota>metazoa>hemichordata Dana\GF14997_Dana_194758495 MACSRFEYVKSFEQDDS--ILP-NVWIVIRVDGKKFHK-FSKTHDFEKP---NDENALNVMNAAATAVMQE--F-RDIVLAYGQSDEYSFVFRKE----------------TTAFKRRSAKLLTYVTSLFSSSYVMQWPRWKSVPLAY---------------------------------------APCFDGRVVLYPS--DENLRDYLSWRQ-ADV--HVNNLYNTAFWKLVLGKGLSNQQAEERLRGTFSADKNELLFQEFGINYNTLP-AMYRKGTILLR-------------------------------------------------------------------------------------krvvdrkgr----QAIVPLHE-----------DLIS--SQFWKNHTEILG- 194758495 Drosophila ananassae eukaryota>metazoa>hexapoda Dmoj\GI17333_Dmoj_195115597 MACSRYEYVKSYEQDDK--ILP-NVWIVIRVDGKKFHK-FANAHKFEKP---NDENALNVMNAAGIAVMEE--F-RDIVLGYGQSDEYSFVFRKD----------------TSAFKRRAAKLLSYVTSMFTSSYVLSWSQWMQRPLSY---------------------------------------APCFDGRIVLYPS--DENLRDYLSWRQ-ADV--HVNNLYNTAFWKLVLESGLTNQQAEERLRGTLSSDKNELLFQEFGINYNTLP-AMYRKGTILLR--------------------------------------------------------------------------------krvvigsqdeqkgr----QAIVPIHE-----------DLIG--NEFWKQHTEILG- 195115597 Drosophila mojavensis eukaryota>metazoa>hexapoda Dwil\GK18307_Dwil_195437103 MACSRYEYVKSYEQDDS--ILP-NVWIVIRIDGKKFHK-FSKIHDFEKP---NDENALNLMNAAAIAVMEE--F-RDIVLAYGQSDEYSFVFRKE----------------TQAFKRRSAKLLTYVTSLFSTNYVMQWPQWMKDRKLNY--------------------------------------APCFDGRVVLYPS--DENLRDYLSWRQ-ADV--HVNNLYNTAFWKLVLDKGLSNQQAEERLRGTFSADKNELLFQEFGINYNNMP-AMYRKGTILMR--------------------------------------------------------------------------------krlelp--qgdktr----QVIVPLHD-----------DLIS--SKFWKTHTELLG- 195437103 Drosophila willistoni eukaryota>metazoa>hexapoda Dper\GL25730_Dper_195155911 MACSRYEYVKTYEQDDT--ILP-NVWIVIRIDGKKFHK-FSKTHNFEKP---NDENALNVMNAAATAVMQE--F-RDVVLAYGQSDEYSFVFRKE----------------TAAFKRRSAKLLTYVTSLFSTSYVMQWSKWMCLPLAY---------------------------------------APCFDGRVVLYPS--DENLRDYLSWRQ-ADV--HVNNLYNTAFWKLVLDSGLSNQKAEERLRGTFSADKNELLFQEFGINYNNLP-AMYRKGTILLR--------------------------------------------------------------------------------krvisdgdndqkgr----QAIVPLHE-----------DLIS--SHFWKVHTEILG- 195155911 Drosophila persimilis eukaryota>metazoa>hexapoda Dpse\GA17959_Dpse_125987191 MACSRYEYVKTYEQDDT--ILP-NVWIVIRIDGKKFHK-FSKTHNFEKP---NDENALNVMNAAATAVMQE--F-RDVVLAYGQSDEYSFVFRKE----------------TAAFKRRSAKLLTYVTSLFSTSYVMQWSKWMSLPLAY---------------------------------------APCFDGRVVLYPS--DENLRDYLSWRQ-ADV--HVNNLYNTAFWKLVLDSGLSNQKAEERLRGTFSADKNELLFQEFGINYNNLP-AMYRKGTILLR--------------------------------------------------------------------------------krvisdgdndqkgr----QAIVPLHE-----------DLIS--SHFWKVHTEILG- 125987191 Drosophila pseudoobscura pseudoobscura eukaryota>metazoa>hexapoda BBOV_IV004960_Bbov_156086032 MANTRFSYVKHFEQDTI--LLP-ESWPVVRVDGRGFTK-FSKLHEFRKP---NEPLALGVMNAAAAHVMST--F-DDIVLAYGHSDEYR-------------------------------KILSSVVSAFSSAFSFYWSRFYPDRQLKI--------------------------------------LPSFDGRIVLYPR--FENIVDYFSWRH-ADC--HINNQYNICFWCLVAD-GKCPDEAYKWLKHTQKGEKNEYIYQSRGINYNNLP-RIFRKGTTLVRllstepglgqcvqdgittkvdrgsidcqatd---------rhrldtsivntrqqatgdvielti------------deseialisqkldsicnp----MGIGVVHC-----------DNTS--EAFWNAAA----- 156086032 Babesia bovis T2Bo eukaryota>alveolata>apicomplexa PAS_chr1-4_0323_Ppas_254566669 MANSKFEYVKAFEKEVY--LLP-ETYMVVRVDGRGFHK-LSETYKFEKP---NDLNALELMNKAAQSVMEK--I-PEALLAYGDSDEYSFLLQKN----------------CEIFERREAKLTSTFSSTFTAYYNFYWKDYFPNSPLTPER------------------------------------LPTFDARVVLYPN--QQNVKDYFSWRQ-VDC--HINNLYNTTFWQLILKKGLTPQESEKRLMGTLSSDKNEILFTELGINYNDEA-EIYKKGTVLIRewelkddqpslnerels----------------------------------------------------------------krqkdrlrkkfrk----AKIQKYHI-----------DIIN--EGFWNERPWLLE- 254566669 Pichia pastoris GS115 eukaryota>fungi>ascomycota DEHA2F22880g_Dhan_294659006 MAKSRFEYVKQFERENF--LLP-DTYLIVRVDGKGFHK-FSEEYEFSKP---NDIRALKVMNNAAKNLMAQ--F-PDIMMAYGDSDEYSFLLRRK----------------CSLFERREMKLVSTFASFISVNYLYEWNLEFPEKQIRLER------------------------------------LPTFDARIVVYPT--IKHIRDYFSWRQ-VDC--HINNLYNTTFWTLVIKGGMTGREAENKLLGTVSSDKNEMLFKEFGINYNNES-EIFKKGTILVReydytr-----egddls----------------------------------------------------------------krqqqrvekqrkk----ASIEEYHL-----------DIIG--DTFWNERPWLLE- 294659006 Debaryomyces hansenii CBS767 eukaryota>fungi>ascomycota DEHA0F24321g_Dhan_50425487 MAKSRFEYVKQFERENF--LLP-DTYLIVRVDGKGFHK-FSEEYEFSKP---NDIRALKVMNNAAKNLMAQ--F-PDIMMAYGDSDEYSFLLRRK----------------CSLFERREMKLVSTFASFISVNYLYEWNLEFPEKQIRLER------------------------------------LPTFDARIVVYPT--IKHIRDYFSWRQ-VDC--HINNLYNTTFWTLVIKGGMTGREAENKLLGTVSSDKNEMLFKEFGINYNNES-EIFKKGTILVReydytr-----egddls----------------------------------------------------------------krqqqrvekqrkk----ALIEEYHL-----------DIIG--DTFWNERPWLLE- 50425487 Debaryomyces hansenii CBS767 eukaryota>fungi>ascomycota GSTEN:00031660:G:001_Tnig_47216813 ----FEY-VRNFETDDS--CLR-NCYIVVRLDGRNFHK-FAEHHTFLKP---NDGRALGLMTHSARCVMAE--LE-DVVIAYGQSDEFSFVFKKT----------------STLFKRRASKLMTHVVSQFSSSYVFYWREFFGDQPLLY--------------------------------------PPGFDGRVVLYPS--NRNLRDYLSWRQ-AD-----------------------SATEENSCEGTLAADKNEILFSEFNVNYNNE--AVHRKGTILIW----------------------------------------erqeetvkkrtkspreeke--------------------------tdatvtrsr----RRVQEHHC-----------DIIG--EQFWQEHPDILE- 47216813 Tetraodon nigroviridis eukaryota>metazoa>vertebrata>actinopterygii THG1_Crei_158283078 MANSKYEYVKQYELDDS--LLP-GCWIVVRIDGKGFTK-FSELHGFEKP---NDKRALDLMDECAKEVLNE--F-PDVRLAYGESDEYSFVLGRS----------------TDMYGRRASKIVSLLVSCFTANYVAKWAAFLPDTPLRS--------------------------------------TPMFDGRAVCYPL--DSNLRDYLSWRQ-ADT--HINNQYNTCFWALV-KSGKTPTEAQATLRGTQTAQKNELLFNEFGINYAHLP-EQFKKGSVVIR----------------------------------------rktmvlqdgev----------------------------------kqradgt------NLPTVLHT-----------DIIR--EEFWQEYPHLMA- 158283078 Chlamydomonas reinhardtii eukaryota>viridiplantae>chlorophyta Dvir\GJ16007_Dvir_195385629 MACSRYEYVKSYEQDDR--ILP-NVWIVIRVDGKKFHK-FANVHKFEKP---NDENALNVMNAAGIAVMQE--F-RDIVLGYGQSDEYSFVFRKD----------------TTAFKRRAAKLLSYVTSMFSSSYVLSWPQWMQQPLSY---------------------------------------APCFDGRIVLYPS--DENLRDYLSWRQ-ADV--HVNNLYNTAFWKLVLESGLTNQQAEERLRGTLSADKNELLFQQFGINYNNLP-AMYRKGTILLR----------------------------------------krlleqe--------------------------------------kgrpvlrer----QAIVPIHE-----------DLIG--AAFWQKHTEILG- 195385629 Drosophila virilis eukaryota>metazoa>hexapoda AT2G32320_Atha_238479416 IGKIEPDYVKSFQFESR--LLP-LTWVVVRIDGCHFHR-FSEVHEFEKP---NDEQALKLMNSCAVAVLEE--F-QDIAFAYGVSDEFSFVLKNK----------------SELYKRQSSKIISAVVSFFTSTYMMRWGDFFPHKKLKY--------------------------------------PPSFDGRAVCYPT--SDILLDYLAWRQ-VDC--HINNQYNTCFWMLV-KSGKSKIQAQDYLKGTQTREKNELLSQQFGIEYNSLP-VIFRMGSSVFR---------------------------------------lktqegvteen-----------------------------------gevsgkqve----AEVGVDYS-----------NIID--QCFWQQHPHILS- 238479416 Arabidopsis thaliana eukaryota>viridiplantae TSTA_094890_Tsti_242778279 MANSKYEYVRLFEQPDN--LLP-ETWIVVRIDGRGFHK-LSDKYKFEKP---NDRRALDLMNAAAVSVMKD--L-PDLIIAYGVSDEYSFVFHPN----------------CQLFERRSAKLVTTIVSTFTASYVCQWPIFFPDKPLDLSS------------------------------------LPTFDGRAVQYPN--AKILRDYMSWRQ-VDC--HINNLYNTTFWSLVLKGGMSNVEAEKELQGTVSSDKNEILFSRFGINYNNEA-EIYKKGSVVYR------------------------------------qyqledqfsattasltqaaesq-----------------psgelsktqqekmrklrrk----AQVVVEHV-----------DIIK--DDFWQRRPWLLS- 242778279 Talaromyces stipitatus ATCC 10500 eukaryota>fungi>ascomycota AaeL_AAEL014040_Aaeg_157138643 MAKSRFEYVKSFEQSDT--LLR-NCWIVVRVDGKGFHK-FCDVHGFEKP---NDNRGLNLMSLAAVNVMQE--F-NEIVIAYGQSDEYSFVFKRD----------------SMVYERRRDKLVSYVASLFTSAYIFNWGYIFKDTVPLKY-------------------------------------PPVFDARAVLYPT--DQNLRDYLSWRQ-ADV--HINNLYNTAFWNLV-ASGLTNAEAENRLRGTLSSDKNELLFSEFNINYNNEP-AMFRKGTVLLK----------------------------------------kktav-------------------------------------------adnksl----SLIVPIFD-----------DMIG--DKFWQTHPELLD- 157138643 Aedes aegypti eukaryota>metazoa>hexapoda THG1_Crog_225711510 MAHSKFEYVRGFESKDT--LLP-NTWLVVRIDGRGFHS-FSDRHDFVKP---NDARSLDLMNAAAKVVMKA--F-PETVLAYGQSDEYSFVFRRN----------------TNLYSRRASKIVTNVTSLFAANYVYLWPEFFPDTKLKY--------------------------------------APSFDGRCVTYPT--DQNLRDYLSWRQ-ADC--HINNLYNTVFWALVQEGGLSNQKAQERLKGTLSGDKNEILFSQFNINYNEEP-QQFRKGSILLK----------------------------------------kkvsvpiegsapvaeeeekqp--------------egggskakr-kphsgtrer----VQVFDLNV-----------DMIG--DEFWRENSHIYT- 225711510 Caligus rogercresseyi eukaryota>metazoa>crustacea OsJ_19344_Osat_222632362 -EKCRYENVKKFDINHR--LPP-CNWTVVRIDICKFEQ-FSLIHSFDKP---NDEAALRLMNASASLMMES--F-PDIVFGYGFSNEYSFVFQDK----------------TELYQRQESLILSSCTSRFTLFYMMKWKDFFPNKDLVE--------------------------------------PPHFEAELLCYPK--QKILCDYLSSRQ-AEC--HTTNQYSTCFWMLV-KSGKSENEAREILKGTLSKDKNELLFQQFHLNYNNEP-AVFRKGSCTYR----------------------------------------qkveesadae-----------------------------------grenttrer----WDVIVAHA-----------D-MG--TEFWRKHPYILR- 222632362 Oryza sativa Japonica Group eukaryota>viridiplantae OsI_20769_Osat_218197162 -EKCRYENVMKFDINHR--LPP-CNWTVVRIDICKFEQ-FSLIHSFDKP---NDEAALRLMNASASLMMES--F-PDIVFGYGFSNEYSFVFQDK----------------TELYQRQESLILSSCTSRFTLFYMMKWKDFFPNKDLVE--------------------------------------PPHFEAELLCYPK--QKILCDYLSSRQ-AEC--HTTNQYSTCFWMLV-KSGKSENEAREILKGTLSKDKNELLFQQFHLNYNNEP-AVFRKGSCTYR----------------------------------------qkveesadae-----------------------------------grenttrer----WDVIVAHA-----------D-MG--TEFWRKHPYILR- 218197162 Oryza sativa Indica Group eukaryota>viridiplantae Dgri\GH10821_Dgri_195030226 MACSRYEYVKGYEQDDR--ILP-NVWIVIRIDGKKFHK-FANTHKFEKP---NDENALHVMNVAGIGVMQE--F-RDIVLGYGQSDEYSFVFRKD----------------TDAFKRRAAKLLTYVTSLFSTSYVMSWPKWMQQPLAY---------------------------------------APCFDGRIILYPS--DENLRDYLSWRQ-ADV--HVNNLYNTAFWKLVLESGLSNQQAEQRLRGTLSADKNELLYQQFGINYNNMP-AMYRKGTILLR----------------------------------------krvlqfgqd---------------------------------------eqdkgr----QAIVPLHE-----------DLIG--AEFWRKHPEILG- 195030226 Drosophila grimshawi eukaryota>metazoa>hexapoda Os05g0535500_Osat_115465113 -EKCRYENVKKFDINHR--LPP-CNWTVVRIDICKFEQ-FSLIHSFDKP---NDEAALRLMNASASLMMES--F-PDIVFGYGFSNEYSFVFQDK----------------TELYQRQESLILSSCTSRFTLFYMMKWKDFFPNKDLVE--------------------------------------PPHFEAELLCYPK--QKILCDYLSSRQ-AEC--HTTNQYSTCFWMLV-KSGKSENEAREILKGTLSKDKNELLFQQFHLNYNNEP-AVFRKGSCTYR----------------------------------------qkvee------------------------------sadae-----grenttrer----WDVIVAHA-----------D-MG--TEFWRKHPYILR- 115465113 Oryza sativa Japonica Group eukaryota>viridiplantae CNC02800_Cneo_58265566 MAKSRFEYVKKFELPDP--LVP-NTYIIVRIDGKGFHK-FSDVHSFDKP---NDIRALKLMNTAAKAVLNE--Y-KDVVMAFGESDEYSFLLRRT----------------TTLYNRRRSKINSSIVSLFTSAYVFHWASFFPNTPLLY--------------------------------------PPSFDARVVLYPN--VKEVRDYFSWRQ-ADT--HINNLYNTTFWALV-HDGLTTAEANKALQGTNSKDKNEILFTKFGINYNTLP-EMFRKGSVCVR------------------slsleepqgslaeqqaahgimtlsivpstsg--------------------------nsntils--qkekvyqgte----GSPMVLHM-----------DIIN--DIFWSERPWLLS- 58265566 Cryptococcus neoformans var. neoformans JEC21 eukaryota>fungi>basidiomycota PICST_60761_Psti_150865885 MAKSRFEYVKQFERENY--LLP-DTYIVIRVDGKGFHK-FSQEYEFDKP---NDIRALNVMNRAAQAVVES--Y-SDVLMAYGDSDEYSFLLRKN----------------CQLYERREMKLITMFSSMISTNYFYFWNEEFPEKKLKQSR------------------------------------LPNFDARAVLYPN--FALIKDYFSWRQ-VDC--HINNLYNTTFWALVLKGGMTPQEAENRLIGTVASDKNEILFSQFGINYNNEP-EIFKKGTIIMR----------------------------------------eldeedsr---------------------------dekelsarqkqridkkrkk----AEIKLLHE-----------DLIT--ETFWSSRPWLSS- 150865885 Pichia stipitis CBS 6054 eukaryota>fungi>ascomycota MONBRDRAFT_31372_Mbre_167518403 MANSRFQYVRGFESEAR--LLP-NTWIVVRIDGRGFHK-FSAKHGFKKP---NDARAINLMNAAAVACMNE--F-PDIVMAYGESDEYSFVFDKR----------------TQLFSRRGDKIMSSIVSFFSSTYVMQWPQHMVDAEGQPEPLVA---------------------------------TPHFDGRCVLYPT--LENLRDYVAWRQ-VDC--HINNLYNTTFWTLVLKGGLTEYEAEQRLVGTFSKDKNELLFSEFGINYNEEP-LMFRKGSVLHW----------------------------------------rripvtvtkegpts---------------------kadptpvv--r--tvtrqk----LTVIVDHC-----------DLLQ--EEFWTEHADVFT- 167518403 Monosiga brevicollis MX1 eukaryota>choanoflagellida THG1_Afim_229366028 MAKSKFEYVRNFETDDS--CLR-NCYIVVRLDGRNFHK-FAEQHKFLKP---NDNRALGLMTRSARSVMED--L-EDIIIAYGQSDEFSFVFKRT----------------STLFKRRASKLMTHVASQFSSSYVFYWKEFFGEQPLLY--------------------------------------PPGFDGRVVLYPS--NHNLRDYLSWRQ-ADC--HINNLYNTVFWTLVQNGGLTTAQAEERLKGTLAADKNEILFSELDINYNTES-AVHRKGTTLIW---------------------------------------ekrdetti----------------------------krtklpkgeetemavtrsr----RRVHAHHC-----------DVIG--EQFWTEHPDILE- 229366028 Anoplopoma fimbria eukaryota>metazoa>vertebrata>actinopterygii LOC587315_Spur_115921197 MAKSKFEYVRQFETQDK--LLP-NSWIVIRIDGKNFHK-FSDIHGFEKP---NDLRGLSLMSEAATSVMTE--F-KDIILAYGQSDEYSFVMKKD----------------TTQYGRRASKLATTVSSLFASSFVFLWPKFFPDTPLQY--------------------------------------PPAFDGRCVLYPS--NKNLRDYLSWRQ-ADC--HINNLYNTCFWTLTQRGNCSPKEAEERLKGTVSGDKNELLFSQFDINYNAEP-EIFRKGTTLMS----------------------------------------ntveeevlcg-------------------------qgtefek------tvsktr----RTVNKIHK-----------DIIG--NSFWTEHPELLG- 115921197 Strongylocentrotus purpuratus eukaryota>metazoa>echinodermata TRIADDRAFT_32525_Tadh_190579964 MAKSRYEYVKLFEQADQ--CLR-NCWIVVRLDGRCFHK-FSSQHRFKKP---NDDRALNLMNHCGKAVMKE--F-PDIILGYGQSDEFSFIFKKS----------------CNLFGRRASKLMTNVTSLFSSSYVFYWKNYFNDILQY---------------------------------------PPTFDGRVVLYPS--DKNLRDYLSWRQ-ADC--HINNLYNTSFWALVQQGQYSLPDAEKKLCGTDSGDKNELLFSQFQINYDKEP-AIFRKGSILLW--------------------------------------------------------------------------------------kenvvrkr----KDVVVEHV-----------DIIG--DNFWTGNSQLLS- 190579964 Trichoplax adhaerens eukaryota>metazoa>placozoa PHYPADRAFT_217293_Ppat_168038223 MANSKYEYVKDFEVNDN--ILP-HTWIVIRIDGRAFTK-FSQAHQFQKP---NDLQALLLMNASAVAVVED--L-ADVVFAYGVSDEYSFVLRKT----------------STLYQRRASKLISVICSLFASSYVMNWGKYFPETKLQY--------------------------------------APAFDGRAVCYPS--ESILRDYLSWRQ-VDC--HINNQYNTCFWNLV-GSGKSTAESQNMLKGTTADVKNNLLFDTFKINYNDLP-QIFRKGSIVYR---------------------------------------------------------------------------kkvekvvkvedgqeikrlr----SCAVVEHE-----------DIIR--DNFWTQYPYILG- 168038223 Physcomitrella patens subsp. patens eukaryota>viridiplantae CpipJ_CPIJ000684_Cqui_170028733 MANSRFEYVKSYEHADI--LQK-NCWIVVRIDGKGFHK-FCDVHNFLKP---NDERGLNLMNLAAVHVLQE--F-NEIVLAYGQSDEYSFIFRRD----------------TSVYNRRRDKLISYVGSLFTSAYIFNWRTIFQDTLELKY-------------------------------------PPVFDSRAVLYPT--DQNLRDYMSWRQ-ADV--HINNLYNTSFWNLV-ASGLTNAEAEERLRGTLSSDKNEILFKEFNINYNNVP-VMFRKGTTLMR-----------------------------------------------------------------------------------kkvkltndkkl----SLIVPIFE-----------DMID--DAFWTRHSEILS- 170028733 Culex quinquefasciatus eukaryota>metazoa>hexapoda PADG_06749_Pbra_226295250 MANSKYEYVKAFEQDDN--LLP-NTWIVVRIDGRGFHR-FSGRYHFQKP---NDERALNLMNTAACAVMKD--L-PDLIIAYGVSDEYSFVFHRN----------------CQLFERRGSKLVTTIVSTFTAHYIYNWSSFFPSAPLEPGF------------------------------------LPTFDGRAVQYPS--VRNLRDYMSWRQ-ADC--HINNLYNTTFWNMILQGGISNTEAEKELQGTVSGEKNEILFSRFGINYNNEP-EMYRKGSVIFR-------------------------------------------------diahlfetqyetqpqteeekagdgsneveaetgee----dheisc----SPMRFIHQP----------EDVP--SEFWTSATVLEP- 226295250 Paracoccidioides brasiliensis Pb18 eukaryota>fungi>ascomycota SPCC63.07_Spom_19075481 MAKSRFEYVKQYERLDR--LLP-ETYIVIRIDGKGFHK-FTKKHDFEKP---NDLRCLNLMNAAARVVMSE--F-TDIVLAYGDSDEYSFVWSKS----------------TELYERRESKLVSHVCSLFTSAFVFNWPKHFDIPLLS---------------------------------------LPSFDGRAVLYPN--MKVLRDYLHWRQ-VDC--HINNLYNTTFWMLILKGGFTNTQAEEYLKGTVSAEKHEILFSKFGINYNFEP-EIYKKGSIWIR-------------------------------------------------------------------------epidqewhqqdkkfsvkqkkk----MVLSILHV-----------SLID--DDFWTSRPFLEV- 19075481 Schizosaccharomyces pombe eukaryota>fungi>ascomycota CD36_70100_Cdub_241957021 MANSKYEYVKLFEKENY--LLP-DTYIVIRVDGKGFHK-FSQFYQFEKP---NDLKALQVMNSAAEKIMSK--Y-SDVMLAYGDSDEYSFLLRKN----------------CQLYERREMKLTTLFASLMSTYYMYFWQQHFPDKPLDIDH------------------------------------LPNFDARAVLYPD--FKHIRNYFSWRQ-VDC--HINNLYNTTFWNLVLKLKMTPQEAEQRLMGTVASDKNEILFKECGINYNNEL-EMFKKGTIIVR-------------------------------------------------------------------efenyeiedetglskrqaqrlekkrkk----ADLKTYHV-----------DIIND-NSWWDNRPWLRD- 241957021 Candida dubliniensis CD36 eukaryota>fungi>ascomycota CAWG_05412_Calb_238883228 MANSKYEYVKLFEKENY--LLP-DTYIIIRVDGKGFHK-FSQFYEFEKP---NDLKALQVMNSAAEKLMSK--Y-SDVMLAYGDSDEYSFLLRKN----------------CQLYERREMKLTTLFSSLMSTYYMYFWSQYFPDKPLHIDH------------------------------------LPNFDARAVLYPD--FKHIRNYFSWRQ-VDC--HINNLYNTTFWNLVLKLKMTPQQAEQRLMGTVASDKNEILFKECGINYNNES-EMFKKGTIIVR-------------------------------------------------------------------efenyetedeaglskrqvqrlekkrkk----AELKIYHV-----------DIIND-DSWWKSRPWLKD- 238883228 Candida albicans WO-1 eukaryota>fungi>ascomycota THG1_Calb_46442219 MANSKYEYVKLFEKENY--LLP-DTYIIIRVDGKGFHK-FSQFYEFEKP---NDLKALQVMNSAAEKLMSK--Y-SDVMLAYGDSDEYSFLLRKN----------------CQLYERREMKLTTLFSSLMSTYYMYFWSQYFPDKPLHIDH------------------------------------LPNFDARAVLYPD--FKHIRNYFSWRQ-VDC--HINNLYNTTFWNLVLKLKMTPQQAEQRLMGTVASDKNEILFKECGVNYNNES-EMYKKGTIIVR-------------------------------------------------------------------efenyetedeaelskrqvqrlekkrkk----AELKIYHV-----------DIIND-DSWWKSRPWLKD- 46442219 Candida albicans SC5314 eukaryota>fungi>ascomycota VITISV_031884_Vvin_147819926 INNIKPEYVKSFQFENK--LMP-LTWIVVRIDGCHFHR-FSEVHEFEKP---NDEQALNLMNSCAVAVLEQ--F-KDVIFSYGVSDEYSFVLKKD----------------SQFYQRRGSEIVTALVSYFTSIYVMKWKEFFPQKELKY--------------------------------------PPCFDGRAVLYPT--SEILRDYLTWRQ-VDC--HINNQYNTCFWMLV-KSGKSKSEAQAYLKGTQAREKNEVLLQQFGIDYNTLP-LMFRQGSSAFW---------------------------------------------------------------------------nevivfsg------kktkq----GESSPPHCLSST-------GYNLC-IQGVSREAFTVLL 147819926 Vitis vinifera eukaryota>viridiplantae TRIADDRAFT_55308_Tadh_196003010 IGNRMKDYERQMEWQ----VQA-NQYYIIRLDGHAFTK-YCQ--GLTKP---FDHRIYLALLHTAADLLNK--F--GSRSAFCFSDEISLVFAPTEPSQRSDREGGEEG--IIHYQGRTMKLCSLTAGMASSRFNYYMNMQQFNDTMGALTLARIQSH-----------------------------PAIFDSRLFCLPN--ADEVVKNIYWRA-HDC--LRNSKISFA------QHHVSRQSLH----RVKASEAIKLVKDKKGINYSLDP-DWFRYGTLIKK----------------------------------------------------------------------ilvdhegingktg--epvkvkrs-----RIAYGSINFTSNL------ASNI--EEILLA------- 196003010 Trichoplax adhaerens eukaryota>metazoa>placozoa GobsU_010100002157_Gobs_168698293 -----KLYEGAESGRRLMPRLP----ALARLDGRAFHA-FVR--GLARP---FDQRLSDLMIDTLAALVRE----TNATVGYTQSDEFTLAWVPFGAGT------------QVFFDGRIQKMTSALAALCSVHFHRRLPAFLPADYTDR--------------------------------------VPTFDCRVWNVPT--FDEAANVFVWRE-LDA--KKNSISMAA------RAYYDHATVH----GRTGAELQELLFRE-GVNWNSYP-ACFKRGTYSRR------------------------------------------------------------------ravrrpftaderdalp---akhqahrt-----PDLVIERSEVAVFNLPPLSKLENR-AGVLFRGEE---- 168698293 Gemmata obscuriglobus UQM 2246 bacteria>planctomycetes Mlab_0759_Mlab_124485581 --------MKDREIFAGLSTTVP---FVLRLDGRSFHR-FSKD-RYKKP---YDKVFSDAMVKTARALVTDSGL--SPSFAYTFSDEISLYVP------------------APVFDCRVEKLASVSAAFAASAFTLYAGASE---------------------------------------------PLAFDARVIPIEE---GLFPAYLSWRQ-AEA--WRNHMNGYAQKILQ-DEGVSPTNAQKQLDGMNAAALHEFAFSR-GVNLALTP-AWERRGICIYR----------------------------------------------------------------------dvvmrdgynpikd--ekvsvertiavi-DEDVPLF------------KPEG--TAWVLSKIR---- 124485581 Methanocorpusculum labreanum Z archaea>euryarchaeota CBFG_01250_Cbac_239627528 IFDELDTGMRRFETSLDQTILP-ELYMAVRIDGRNFTK-LTKETCRFEAP--FDIRFRDAMVDTVKHLMDC-GF--RIVYGYTQSDEISLLFHPR----------------DNTFGRKTRKINSVLAGEASAFFSLRLGV-----------------------------------------------LACFDCRVVPLPN--LECVKDYFSWRQ-EDA--HRNSLNAHCYWLLR-REGMSAREATREIEGKSIAFKNELLFSR-NINFNDLP-NWQKRGVGLYY----------------------------------------------------------------------ssyekkgynpvkd--eevtsirsr-----LDVNMDLEIG--------EAY---TDWVISQIP---- 239627528 Clostridiales bacterium 1_7_47FAA bacteria>firmicutes METSMIF1_02117_Msmi_261349483 --------MKEYEVYGDL-KVPVNSKIILRLDGRSFHS-LAKNLNLEKP---YDEDFARLMVKVSKDLFNE--F--APVFIYTFSDEISILLD------------------NIPFNGRIEKINSVVASFASSSFTYNLNKEIAK-------------------------------------------PIAFDSRIIPIID---DDIYKYFKWRQ-DEA--WRNCINAYGIHILK-SK-YGDKVANEKIKGLKSSDIHELLFGE-GINLNDVD-NWKKRGIAIYK----------------------------------------------------------------------qdkeiagynkken--knqvsyrsflfa-DLEIPIFS-----------E------NFFKDINII--- 261349483 Methanobrevibacter smithii DSM 2374 archaea>euryarchaeota METSMIALI_01627_Msmi_222445979 --------MKEYEVYGDL-KVPVNSKIILRLDGRSFHS-LAKNLNLEKP---YDEDFARLMVKVSKDLFNE--F--APVFIYTFSDEISILLD------------------NIPFNGRIEKINSVVASFAASSFTYNLNKEIAK-------------------------------------------PIAFDSRIIPIID---DDIYKYFKWRQ-DEA--WRNCINAYGIHILK-SK-YGDKVANEKIKGLKSSDIHELLFGD-GINLNDED-NWKKRGIAIYK----------------------------------------------------------------------qdkeivgynkken--knqvsyrsflfa-DLEIPIFS-----------E------NFFKDINII--- 222445979 Methanobrevibacter smithii DSM 2375 archaea>euryarchaeota Msm_0463_Msmi_148642523 --------MKEYEVYGDL-KVPVNSRIILRLDGRSFHS-LAKNLNLEKP---YDEDFAGLMVKVSKDLFNE--F--APVFIYTFSDEISILLD------------------NIPFNGRIEKINSVVASFAASSFTYNLNKEIAK-------------------------------------------PVAFDSRIIPIND---KDIPKYFKWRQ-DEA--WRNCINAYGIHILK-SK-YSDKTANEKIKGLKSSDIHELLFNE-GINLNNVD-NWKKRGIAIYK----------------------------------------------------------------------qnkeivgynkken--knqvsyrsflfe-DFEIPIFS-----------E------NFFKDINII--- 148642523 Methanobrevibacter smithii ATCC 35061 archaea>euryarchaeota Mhun_0610_Mhun_88601907 ----MKEREIYADLRTLS-------PIIVRLDGRAFHQ-VTDKLNFSEP---FDEKFSDAMAFVTKGLLIDAGF--APVFGYTFSDEISLYFS------------------ENLFLGRVEKIDSVLASFASSCLTIALELKE---------------------------------------------PVSFDARIIPVTP---DHILPYLSWRQ-QEA--WRNHMNGWSQKLLK-DEGYTSQEAASMLHAMKAAELHEFCFQR-GVNLAMTP-AWQRRGILVYR----------------------------------------------------------------------tvvekegynpktd--ektittrrvvti-DKDIPLFGKP---------D-G---KDFIERIII---- 88601907 Methanospirillum hungatei JF-1 archaea>euryarchaeota AM1_3767_Amar_158336895 KFTELDQTLRIYETAYDLCVLPGL-QMVARLDGRNFTR-LTKETLDLDAP--FDATFRDAMVATVQHLMTC-GF--RVLYGYTQSDEISLLLHPD----------------EQTFARKLRKLNSVLAAEASAKMSLILGC-----------------------------------------------IAVFDCRISQLPT--VDLVVDYFRWRQ-EDA--HRNALNAHCYWMLR-KGGDSAGTATQKLEGLSVGDKNELLFQLGQMNFNDLP-PWQKRGVGCLW----------------------------------------------------------------------qsyptagvnpvsg--evsqgtrtkigv-ELNLPMK------------EHY---REFILKRMTL--- 158336895 Acaryochloris marina MBIC11017 bacteria>cyanobacteria Cpin_5797_Cpin_256424765 KFDELDSKMRIYETAHDKIVLPGM-YMVARIDGRGFTK-LTKETHPFEAP--FDPRFRDYMVETVKHLMNC-GF--NVVYGYTESDEISLLFHLA----------------EGAFGRKHRKYNSILAGETSAKFSLQLGS-----------------------------------------------LAAFDCRICELPN--RQLVVDYFRWRN-EDA--HRNALNAHCYWQLR-RDNHTRQSATNTIEKMSTADKNELLFRY-NINFNNLP-AWQKRGIGIYW----------------------------------------------------------------------aemekdgvnpktg--eqvvvkrrqlyv-NQELPVK------------DEY---NRFIQELIE---- 256424765 Chitinophaga pinensis DSM 2588 bacteria>bacteroidetes/chlorobi MC7420_6362_Mcht_254412407 KFDQLDAKMRVYETAHDHCVLPGL-YMIARLDGRGFTR-LTRDVHPFSAP--FDQRFRDAMILTVEHLMNC-GL--KVIYGYTQSDEISLLVDGA----------------ESAFNRKLRKLNSVLAGEASAKFSLILGT-----------------------------------------------VACFDCRISQLPT--IEIVVDYFRWRQ-EDA--HRNALNSHSYWCLR-NDGKTARQATSQLERLSVADKNELLFQH-GINFNHLP-NWHKRGVGLYW----------------------------------------------------------------------etyqkpglnpltg--etteatrkrirq-ELNLPMK------------DQY---SQFIRDVLS---- 254412407 Microcoleus chthonoplastes PCC 7420 bacteria>cyanobacteria MM_1964_Mmaz_21228066 ----MKNREIYAEMRCIP-------PVVLRADGRNFKN-TLSGLGFEKP---YDTTFARAMADTAELFIKKSGL--SPYFAYTFSDEISFLFT------------------DLPFDGRVEKIDSVVASFLGSALTIKLRLEA---------------------------------------------PIAFDSRLVALQK---EEVSEYFHWRQ-LEA--WRNFVASWGYYSLR-NEGMGKDEAGKFLKGKKEWEIHEMLFER-GINLAALP-AWQRRGIIISK----------------------------------------------------------------------eeceisgfnpvyg--kevksmrrritq-NWEIPKFKS----------EEG---ITFLEKLIN---- 21228066 Methanosarcina mazei Go1 archaea>euryarchaeota Mmah_1763_Mmah_294496410 ----MKEREIYADLRCVS-------PVILRVDGRNFQR-ALKKEGFQKP---YDIFFATCMADSIELFFKKSNM--NPVFAYTFSDEASLVFT------------------DLPFDRRVEKLDSVVPSFLSSAFTLFSGIEE---------------------------------------------PVAFDCRVIPVCN---DQFTEYMHWRQ-QEA--WRNFVSSYGYYTLI-DEGIDRKSAASVMHGKKSQDIHEMMFER-GTNLAKKP-AWQRRGVAVYR----------------------------------------------------------------------ekypiegynplle--ektqstrtrisq-DWDLPLFSTA---------EG----DNFLKRHIS---- 294496410 Methanohalophilus mahii DSM 5219 archaea>euryarchaeota MCP_2556_Mpal_282165226 ---MADERFKVKEIYKDIKALP---PVIVRADGRNFKE-SLARLKFKKP---YDLKFEKAMVSAGHALMESSGL--GPLWVYTFSDELNVFFS------------------ELPFDGRVEKLDSVVPSFLSSALTLALGVKT---------------------------------------------PLAFDARVVPLHY---EDVSGYLQWRQ-AEA--WRNHMQSYGFYTLV-KDGLAEKYASKTLRGMKFEDIHEMMWQR-GINLNETP-AWQRKGVFIYR----------------------------------------------------------------------kmvmkegydpvkk--kkvrarraevee-SWDPPLFGSK---------EG----EAFLKSLKS---- 282165226 Methanocella paludicola SANAE archaea>euryarchaeota HMPREF0198_1468_Chom_258545199 KFDDLDRLMRPYETAYD-FCVPMGNHIVVRLDGRGFTR-LTKDIWQFDAP--FDPRFRDLMTQTVAHLMQC-GF--NILYGFTQSDEISLLFHPA----------------DDTFARKTRKLASVLAGEASASFTHLHGQ-----------------------------------------------MATFDARVCVLPG--AAQVWDYFHWRQ-EDA--HRNALNAHCYWKLR-QEGASERDAAARISGLKLAEKHDLLHAR-GINYNDLP-AWQKRGIGLYW----------------------------------------------------------------------rdvaqsghnpqtg--eatqttrrrlit-DLELPYK------------EDY---RRFLQGMTAVA-- 258545199 Cardiobacterium hominis ATCC 15826 bacteria>proteobacteria>gammaproteobacteria Mbar_A1746_Mbar_73669253 --------MKDREIYAEMRCIP---PVVVRADGRNFKN-TLRDLGFGKP---YDQTFARAMADTAELFIKKSGL--SPLFAYTFSDEVSFLFM------------------ELPFEGRVEKMDSVTASFLGSALTINLQFEK---------------------------------------------PVAFDSRIVVLQK---DEIPAYFHWRQ-LEA--WRNFVAAWGYYTLL-NEGISKIEASKYLRGKKEWEIHEMLFER-GINLAKIP-AWQRRGVIISK----------------------------------------------------------------------eeyeisgfnpvld--ketkslrrriiq-NWEIPNFKS----------EEG---MEFLQKLIN---- 73669253 Methanosarcina barkeri str. Fusaro archaea>euryarchaeota Mboo_2270_CMet_154151809 --------MDNYEIFSNLATIP---PVFVRLDGRAFHG-LTKKYGFAKP---FDDRFCSAMMAACRALVADSGL--APVFAYTFSDEISLYFT------------------GLPFSGRVEKIDSVAASYAASALTLALGAEE---------------------------------------------PLAFDARVVQATP---ETAIEYMTGRQ-DEA--WRNHINSYCQQALI-AEGMDATDAARKLKGLPAAELHEMMHER-GVNLAKTP-AWQRRGILVCK----------------------------------------------------------------------keeekegynpvtd--ehvivtrsrvve-DRDLPLFSTP---------EG----QAFLRMLIC---- 154151809 Candidatus Methanoregula boonei 6A8 archaea>euryarchaeota Mpal_0151_Mpal_219850835 -------MNDNREIFSTLAAIP---PLWVRLDGRAFHS-LTADLALERP---FDLRFSEAMATTASALVGSSGL--SPCFAYTFSDEISLYFT------------------GLPFGGRVEKIDSVAASYAASALTLALGVTS---------------------------------------------PLSFDARVVFATP---TSAREYLINRQ-QEA--WRNHINAYCQHALI-SEGLSSREAASRLRGLPGKALHDLMHER-GVNLAETP-AWHRRGLMART----------------------------------------------------------------------advtvsgynpikk--ekvttirhrvvi-DRDLPLFSSP---------EG----EVFLSSVLG---- 219850835 Methanosphaerula palustris E1-9c archaea>euryarchaeota M23134_06676_Mmar_124008957 KFDELDARMRVFESANDFLVLP-NVYMVARLDGRGFTKLTKQNLSLETP---FDVRFKDYMIATTTHLMTC-GF--KFVYGYTESDEISLLFDLE----------------ENIFGRKTRKLVSLLAAEASAKFALLINQ-----------------------------------------------VASFDCRISQLPR--KQDVIDYFRWRN-EDA--FRNSLNAHCYWMLR-KQGISAKKANDEVTGLSVAQKNELLFQA-GINFNDLP-LWQKRGVGFYW----------------------------------------------------------------------ttidkegfnprtn--ekvtaqrktlvt-NDALPMK------------DAY---SNFLTDLL----- 124008957 Microscilla marina ATCC 23134 bacteria>bacteroidetes/chlorobi Mthe_0875_Mthe_116754185 PESSMIRRGRRNEAIY--SELRVRSPFFVRVDGRGFGR-MLRD--FSKP---YDLGFARSIVSAARAFMESSGL--APILAYTFSDEINLLFL-D----------------EP-FRGRLEKLDSITASYISSSLSISLGR-----------------------------------------------VVSMDARVIPICR---EEILSYLQESQ-AEA--WRNHVFSYGFYALL-GEGKSHADAMESLRNMKESDIHEMLFQR-GVNLAKTP-AWERRGVMVYR----------------------------------------------------------------------sg--------------------sgive-EWELPLFTT----------DDG---RIFMEEILS---- 116754185 Methanosaeta thermophila PT archaea>euryarchaeota NEIFLAOT_00025_Nfla_225075026 RFDDLDKRLRQYETAYD-FCVPQENFIVVRLDGRGFTR-LTKEIWQFEAP--FDIRFRDLMAHTVQHLMKC-GF--NVAYGYSESDEISLLLRRD----------------DDTFKRKTRKIISVLAGEASAAFSVAHGQ-----------------------------------------------PAAFDARVCVLPN--EKLVVDYFRWRH-EDA--HRNALNAHCYWMLR-KKGESVSRATEAVSGLTRAQKHDLLFEH-NINFNELP-AWQKRGFSVYF----------------------------------------------------------------------qttlkegfnpqtg--etaqaerqilht-DFELPLG------------DDY---GAFVLERIG---- 225075026 Neisseria flavescens NRL30031/H210 bacteria>proteobacteria>betaproteobacteria NEIELOOT_02904_Nelo_294671196 RFDDLDKRLRQYETAYD-FCVPQENFIVVRLDGRGFTR-LTKEIWQFEAP--FDIRFRDLMAHTVRHLMQC-GF--NVAYGYSESDEISLLLRRD----------------DDTFKRKTRKIISVLAGEASAAFSVAHGQ-----------------------------------------------PAAFDARVCVLPN--EKLVVDYFRWRH-EDA--HRNALNAHCYWMLR-KKGESVSRATDAVSGLTRAQKHDLLFEN-GINFNELP-AWQKRGFGVYF----------------------------------------------------------------------qttlkegfnpqtg--etvqverpilqt-DFELPLG------------DDY---GAFVLERIG---- 294671196 Neisseria elongata subsp. glycolytica ATCC 29315 bacteria>proteobacteria>betaproteobacteria NEISICOT_02755_Nsic_255067726 RFDDLDKRLRQYETAYD-FCVPQENFIVVRLDGRGFTR-LTKEIWQFEAP--FDIRFRDLMAHTVRHLMQC-GF--NVAYGYSESDEISLLLRRD----------------DDTFKRKTRKIISVLAGEASAAFSVAHGQ-----------------------------------------------SAAFDARVCVLPN--EKLVVDYFRWRH-EDA--HRNALNAHCYWMLR-KKGESVSRATDAVSGLTRAQKHDLLFEN-NINFNELP-AWQKRGFGVYF----------------------------------------------------------------------qttlkegfnpetg--etvqaerqilht-DFELPLG------------DDY---GAFVLERMV---- 255067726 Neisseria sicca ATCC 29256 bacteria>proteobacteria>betaproteobacteria PlimDRAFT_36970_Plim_229539832 ----LDNKMRVFETAADLCVLP-GMFMVARLDGRSFTRLTKDVCPFEAP---FDERFRDLMVSTTESLMNC-GF--RVLYAYTQSDEISLLFDLE----------------EQLFGRKLRKYNSLLAGEASAQFSLKLRQ-----------------------------------------------PACFDCRISQLPT--SELVVDYFRWRN-EDA--ARNALSAWCYWTLR-KDGQNEQQATKRLLGLSVSQKNQLLFQS-GINFNDLP-NWQKRGVGFYW----------------------------------------------------------------------eeydrpamnpitk--ekvtarrrrlht-DFDLPMQ------------DDY---SQFVRLIVS---- 229539832 Planctomyces limnophilus DSM 3776 bacteria>planctomycetes sce6062_Scel_162454339 ----MDAKMRLGECFHELRALR-GAYIVIRVDGRSFSR--LTERTCEKP---FDTGFHHKMTDAAKAVLTS--L--HGSYAHTQSDEISILLPHE----------------TDVFDREVEKLVSIAASSATASLSLALSA-----------------------------------------------PVEFDARLWLGAR--RGDVVDYFRWRQ-ADA--ARSALNNWCYWTLR-KEGKSVKEATHAMLGLSVAGKNELLFSR-GINFNDVP-AWQRRGSGVYW----------------------------------------------------------------------etiakvgrnprtg--esvpatrrrlrv-DDDLPMK------------EAY---GKFVRQFLK---- 162454339 Sorangium cellulosum 'So ce 56' bacteria>proteobacteria>deltaproteobacteria DDB_G0289191_Ddis_66805193 GNFRNYNKFELFEKAFDFVLPP-YQPIIIRLDGNSFSK-LNKQLKLER----HDERFHESMKETSNNLFSH--F-IGCKFIYSFSDEINVIYNQYPD--------------NQFLSNRIQKLISTTSSITSLNFSINLSKKLNNNNNNNNV------------------------------------FSYFDCRAFVLPI---NEVKDYFIKRQ-GRC--YTNFLSSIASDNGF-NTDSIFNNDSGLDGSKKNIYAREYYLEKQGVNLMEIP-EHFKSGFLVFK-----------------------------------------------------------------------nst-----------nel----------DYKSPI-------------NFKS--DTIIDYLLN---- 66805193 Dictyostelium discoideum AX4 eukaryota>amoebozoa>mycetozoa>dictyosteliida Mbur_1464_Mbur_91773429 --------MKKREVYSDLRCIP---PVIVRLDGRTFKH-TLSRLGCEKP---YDEKFASAMADSLELFFKKSGI--NAALAYTFSDEASILFF------------------DLPFDGRVEKLDSVISSYLSSAFTIKMGLDE---------------------------------------------PVSFDSRIVPVNK---ENVAEYLIWRQ-SEA--WRNCVSSYGYYTLL-SEGMSKKDAASAIKGKKAQGIHELLFQR-GINLDKVP-MWQRRGILVYK----------------------------------------------------------------------ddykvagfdpvrk--eirsstrnkvvq-DWEVPQFSS----------EEG---TDILKKHLI---- 91773429 Methanococcoides burtonii DSM 6242 archaea>euryarchaeota CMU_028750_Cmur_209557756 MACSKYEYVKSYEQS--NRVLN-NSWFVVRIDGCSFHE-FTRSHNYDKP---NDKNGLDLMNRAAESVMRK--I-SDIIIAYGQSDEYSFVFRRK----------------TDLWGRRYEKILTYTVSLFTSSFVYYWDNFFPKIRLTY--------------------------------------PPTFDGRIIIYPT--DKDIRDYLSWRQ-VDC--HINNLYNTCFWALVKFRNISEKEATELLKHSVSSDKNELLFSEFNINYSKIP-KQFRKGTVLYR--------------------------------------------------------------------prqktkktsseyfn------mrdird----KEVMIDDTKDSSDE-----DIT---HPIWSCK------ 209557756 Cryptosporidium muris RN66 eukaryota>alveolata>apicomplexa C4_0009_Uarc_82617278 ---MRVSDFKDHEIYADLRATP-P--VVIRVDGRSFKN-LLRKHRFEKP---FDHRFASAMADATESFFQQSGL--YPVVAYTFSDEINILFR-D----------------ALPFDGRIEKLVSVVPSSISSALTRSLKIS----------------------------------------------PIAFDGRVIPLHP---EQIIEYLVWRQ-AEA--WRNCINGYGYYTLR-SNGLSGKDAASRMRGLRASDIHELCFQH-GINLGKVP-LWQRRGVLVYW----------------------------------------------------------------------eeytkpgydpvrd--ievvvnrkrvaq-NWDLPIFAS----------DEG---GVMVRGLVG---- 82617278 uncultured archaeon archaea BSM_02380_Uarc_268323173 NQARHKYKAQDREIYANMKAIT---PLFVRADGRNFKR-VLST--FGKP---YDARLAKGIVKAVELLFLNSGF--NPKLAYIFSDEINLYFD------------------DVPFKGRIEKLDSVLASFLASALTIILDFKD---------------------------------------------AIAFDARVIPVCG--EADVLEYLAQRQ-AEA--WRNHINAYGYYGLQ-DTGLSEKEAEKRLKGMKAAEVHEMLFRL-GINLNETP-KWQRRGILIAR----------------------------------------------------------------------qrhekegydpkla--ekvtatrykvvs-LWDPPLFGS----------EEG---RNLIHQLLD---- 268323173 uncultured archaeon archaea MTH972_Mthe_15678990 ---MAGHSMREHEVYSKLRAPP-SSRMILRLDGRGFHR-LTESLDFDRP---YDEAFRDLMIRTCLDLMEE--F--SPSLIYTFSDEINVLLD------------------SVPFAGRVEKLDSVFSGFASSSFTRGALEAGFSPVK----------------------------------------PVSFDCRVIPISS---DIVPEYFRSRQ-DES--WRNCLNSYAYWTLR-RE-VGARRAAERLRGLKSDSLHDLLFER-GVNISRVP-AWQRRGVGVYR----------------------------------------------------------------------vphrvrgynpird--eevsavrmrvkv-DLELPLFTD----------EFF---EGLMR-------- 15678990 Methanothermobacter thermautotrophicus str. Delta H archaea>euryarchaeota MXAN_5968_Mxan_108762077 -----AARARQGEVFHGQRMLP-GAWVVLRVDGRGFSR-FTEA-RYEKP---FDPVFHQFMVRTASVMLEE--L--QGVYAYTQSDEISVLFRPD----------------WALFDRSVEKVVSLAAGLASATFTHAAGV-----------------------------------------------PAVFDGRAWLGAS--ERAVLDYFIWRQ-ADG--SRCSLHGWCYWTLR-KEGRSAAQATRELDGKPVSYKNELLFQR-GINFNDVP-LWQRRGSGVWW----------------------------------------------------------------------eayqkegvdprdg--rrtqtlrrrlkv-DSELPMK------------EAY---EHLVRGLLA---- 108762077 Myxococcus xanthus DK 1622 bacteria>proteobacteria>deltaproteobacteria Haur_4049_Haur_159900562 NFDQLDQLMRVYETSNDLAVLP-NIWMVVRIDGRSFTR-LTKDVMNYQRP--FDQRFRDAMISTVKHLMEC-GF--PVLYGYTQSDEISLLLSYG----------------CDVFGRKLRKYCSILAGESSGHFSLAIQQ-----------------------------------------------QASFDCRICQLPT--DQLVHDYFRWRM-EDA--HRNALNAYCYWSLR-DQALDPQTAHMQIANLSVAQKNELLFQH-GINFNDLV-AWQKRGIGFYW----------------------------------------------------------------------qtvdlqgynpqtq--mptltkrrkihv-EFELPQR------------EAY---SNLLASHFD---- 159900562 Herpetosiphon aurantiacus ATCC 23779 bacteria>chloroflexi BGP_4908_Bsp._153872919 ----FDARMRIFETTHDCCVLP-CLYMVARLDGRNFTR-LTKEVCQFEVP--FDFKFRDIMIETVKHLMNC-GF--RINYGYTQSDEISLLFHL----------------NEDTFNRKLRKFNSVLAAEASAKFSLLLGK-----------------------------------------------MGCFDCRISQLPT--KELVSDYFRWRQ-ADA--QRNALNAHCYWLLR-KEGQSQQDATTALLNLSVSDKNEFLFSH-GVNFNELP-HWQKEVLVYIGklmknkp--------------------------------------------------------------------------------------------------------------------------------- 153872919 Beggiatoa sp. PS bacteria>proteobacteria>gammaproteobacteria thg1_Ppal_281212173 MANSKYEYVKLFEQP--DSLLP-NVWIVVRVDGRGFHK-FTQKHDYAKP---NDDRGLNLMNRCALEVCKE--F-TDIVIAFGESDEYSFVLKKQ----------------CTLFERRESKITSSIVSYFSSQFVYRWKEYFGDFELKY--------------------------------------PPTFDSRAVCYPS--DQNLRDYLSWRQ-ADT--HINNLYNTAYWALVLKGGKTPNEAELELRGTLSEQKNEILFSRFAINYNNLP-QMYRKGSVIYR--------------------------------------------------------------------kmvaeeridsrtgq------pttkqkk---RPITKRTKNNDFGGKNRKKKGD-VDDDVWLWSTIAG-- 281212173 Polysphondylium pallidum PN500 eukaryota>amoebozoa>mycetozoa>dictyosteliida MK0093_Mkan_20093533 ---MKPVEPCDLEVYADLRVPP-NTHLVLRIDGRAFTK-LTRRLGLKKP---YDRRFAEAMAETAVRMIRDAGL--GITLVYTFSDELNALIPRG----------------NVPFSGRVEKLTSVSASCASTYFFRALQRHGIDPTGE---------------------------------------TVSFDSRCVVLTD---DDLVDYFKWRQ-DEA--WRNHLNSYAYWALR-ERGLKPKEAAERLRGMKAHDVHELLYREFGINLGRTP-AWQRRGILAYR----------------------------------------------------------------------vavnedgvq------------rrrvtr-DWAPPFFD-----------ESE--GERLIRACASQGY- 20093533 Methanopyrus kandleri AV19 archaea>euryarchaeota Msp_1147_Msta_84489939 ---------KEYEQFSQLKIVP-DLPIIIRIDGRSFSK-YTKQLGLKKP---FDKRLRNLFIEVSKDVLGE--F--NTKYIYTFSDEINILLE------------------QIPFNGRVEKIDSVICSYVSSSFMKHLFISKDQFDVDITTLK----------------------------------PASFDSRII-ITS---KNIKNYFKWRQ-DES--WRNCLNSYAQYVLN-KE-NKPEKTAKILYKLNKSNIHEL-LFNHGINIAHVP-TWQKRGVAIYK-----------------------------------------------------------------------------------ikeekegynpklk---KKTLYFR-----------N-----KVYVDKNISLFK- 84489939 Methanosphaera stadtmanae DSM 3091 archaea>euryarchaeota Memar_0402_Mmar_126178352 ------MDTKDREIFSTLTIFP---PVFVRLDGRAFHR-LARARNLKKP---FDPAFNESMRAVCRYLLTGSGL--SPAFAYTFSDEISLYFN------------------ALPFSGRVEKLDSVTAAVAASKLTIELGCTE---------------------------------------------PLAFDARTIPAAG---EFAVEYLVSRQ-NEA--WRNHINAYCQNALV-EEGMTAREAAAMLRGMQSEAMHEMMFER-GVNLAATP-AWQRRGTLLYR----------------------------------------------------------------------decikegynpltg--etveavrtcire-PEETPLFSTP---------E----GEALIRSLTG---- 126178352 Methanoculleus marisnigri JR1 archaea>euryarchaeota Kcr_1204_CKor_170290817 NASIRSVDWKEREIFSGLR-VPSDAPVMVRIDGWRFHK-VADELGLERP---FDRRLIEALIQAPLSLMRM-GF--PLALSFAFSDEISFLIY-----------------PPIPWSGRVEKLISVIPSHSSAIVSMFLNY-----------------------------------------------PVCFDARIIILRD--LDEILGYLSWRQ-SEA--WRNALNSYALFALE-RSGMNREDAVKELRNRKADSLHDIIFTKLGINIATVP-SWQRRGVIVRK----------------------------------------------------------------------------ryeekhc--esgkvvrrvpev-DWDIPLFSTP---------E----GRDYLLEALR---- 170290817 Candidatus Korarchaeum cryptofilum OPF8 archaea>korarchaeota thgL_Mrum_288559933 --------MKDYEIYSNL-KVPKNSNIILRLDGRKFHS-LAKYLALKKP---YDNNFAKLMSEVSLDIFNQ--F--SPKFIYTFSDEISILLD------------------EIPFLGRVEKLNSVFSSIASSSFTYHLLNDFKDDFNMDKLDEDDRNVIF---------------------------PVSFDSRVIPIVN---EDIYDYFKWRQ-DEA--WRNCVNAYGIWALK-KE-FSPQIANEKIKGLKSSEIHELLFKK-GINLNDVD-TWKKRGIGIYK----------------------------------------------------------------------ienqiegfnpvke--ektvsyrsevfv-DYELELF------------N----KEYFYRF------- 288559933 Methanobrevibacter ruminantium M1 archaea>euryarchaeota RCIX455_Umet_147920979 --------MKRKEIFSAIRAAP---PVIVRLDGRNFKE-SLSRLGFAKP---YDLRFQQGMVAAARMLVEQSSL--APEWAFTFSDEVSLLFK------------------KLPFDGRLEKLDSVIPSYMASALTIALKVET---------------------------------------------PLAFDSRVIPVHP---EEIPEYLAGRQ-AET--WRNHMQSYGFYTLV-SEGMGEKEAAAKMKGMKFEDIHEL-MWQRGVNLNETP-GWQRKGVFIYR--------------------------------------------------------------------kk--ttregynplkg--ekvsverrev-v-EDWDPSLFTS---------DE---GKAYLKQALG---- 147920979 uncultured methanogenic archaeon RC-I archaea>euryarchaeota Arcpr_1732_Apro_284162827 RQKRKRINWKDRELYAHLLAPN---VFILRIDGRNFTN-VLKD--FEKP---YDIRFARAMVETCREIMRE--F--NPAFAYTFSDEVSFLFR------------------D-LFGCRVEKIDSIIASEFSSRLSLKLGF-----------------------------------------------PVSFDSRIIYASF---DEISDYLKSRQ-DEC--WRNHINSYAFYTLL-KEIKDRRKTQEFLSGKKSSEIHDLLFER-GINISKTP-AWQRRGIMLYW----------------------------------------------------------------------eevefe--kefeg--rkvrfkrrrive-EWNLPLFDS----------ED---GKKLIEKVLS---- 284162827 Archaeoglobus profundus DSM 5631 archaea>euryarchaeota CTRG_05039_Ctro_255731634 MANSKYEYVKLFEKE--NYLLP-DTYIIIRVDGKGFHK-FSQFYQFEKP---NDLKALQVMNKAAEKIMQK--Y-SDIMLAYGDSDEYSFLLRKN----------------CDLYERREMKLTTLFASLMSTFYMHFWQQIFPDKPLVIDH------------------------------------LPTFDARAVLYPS--FKHIRDYFSWRQ-VDC--HINNLYNTSFWSLVLKLNMTPQEAEQKLMGTVSSDKNEILFKECGINYNNEL-EIFKKGTIFVR-------------------------------------------------------------------efenyevqietglssrqkqrlekkrkk----AQVKEYHV-----------DIIN--NSWWETRPWLEE- 255731634 Candida tropicalis MYA-3404 eukaryota>fungi>ascomycota LELG_05489_Lelo_149234768 MANSKYEYVKAFERE--NYLLP-ETYIVIRVDGKGFHK-FSKYYDFAKP---NDLGALQVMNAAAMQIMHR--Y-SDVLLAYGDSDEYSFLLRRS----------------CELYERREMKLCTLFASLMSTYYMHFWNQRYPEKLIQLEM------------------------------------IPTFDARAVTYPN--FRTVRDYFSWRQ-VDC--HINNLYNTTFWNLVEKLGLTGQEAENKLMGTVSSDKNEILFKECGINYNNEP-EIFRKGTIFVR---------------------------------------------------------------eivnskecndddeikqlsarqkqrqekmrkk----AEIKEYHV-----------DIIN-DDNWWESRSWLER- 149234768 Lodderomyces elongisporus NRRL YB-4239 eukaryota>fungi>ascomycota At2g32330_Atha_3831463 KDIGKIEPDYVKSFQFESRLLP-LTWVVVRIDGCHFHR-FSEVHEFEKP---NDEQALKLMNSCAVAVLEE--F-QDIAFAYGVSDEFSFVLKNK----------------SELYKRQSSKIISAVVSFFTSTYMMRWGDFFPHKKLKY--------------------------------------PPSFDGRAVCYPT--SDILLDYLAWRQ-VDC--HINNQYNTCFWMLV-KSGKSKIQAQDYLKGTQTREKNELLSQQFGIEYNSLP-VIFRMGSSVFR----------------------------------------------------------------------lkv--------------------------LLLLSYS-----------K-----ILLVFHSRFLLL- 3831463 Arabidopsis thaliana eukaryota>viridiplantae RCOM_0075620_Rcom_255578805 IGKVNPDYIRSFLFK--SKLLQ-STWVVIRIDGCHFHR-FSDGHEFEKP---NDEQALNLMNSCAVAVLKE--F-QDVVFAYGVSDEYSFVLKKD----------------SKFYSRQASDIVSVIVSFFSSMYVMNWKSFFPQKDLKY--------------------------------------PPSFDGRAICYPS--SEILQDYLAWRQ-VDC--HINNQYNTCFWALV-KSGKSKTDAQSTLKGTQVREKNE-ILAQFGIDYNNLP-LIFRQGSSVFR--------------------------------------------------------------------------vkedivihe--ngasaknlr----TKFL---------------EGAP-RHPWLEAPATRTN- 255578805 Ricinus communis eukaryota>viridiplantae Pars_0105_Pars_145590370 ------MRYREREVV----CEPASPPFAVRLDGVGFGS---RLRDFPHP---RSRLVHNALVEVARTIAQT--Y--GADFAHVVSDEINLFFHR-----------------LAPYGGRTFKIISVLAGHASAEATALLGR-----------------------------------------------PLYFDGRVVKLRD--LCDAATYFLFRA--RV--GLNNYAVQIA-----RGMGLLRERTPPIGEVVAKIKIDDYELAWGAFLEKER-G-YRKEVDVCN-------------------------------------------------------------------------------------------------aLSSLCNVC------------------------------ 145590370 Pyrobaculum arsenaticum DSM 13514 archaea>crenarchaeota Tneu_0097_Tneu_171184582 NPRHLEMRYREREAV----CEPASPPVAVRLDGVGFGK---RLRDFPAP---RSRLVHTAIAEVARDLAAQ--H--GAEFVHVVSDEINLLFL-----------------TSVPYGGRTFKLASVLAAQAAAGVTAKLGR-----------------------------------------------PLYFDGRVVKLAD--RCDAARYVLFRA--RV--GLNNYVVKAA-----RLAGVVGAETPRIEELLAAVKIEDFELAWGSFMYKEG-G-YARSGDLCN-------------------------------------------------------------------------------------------------aVSQLCEMC------------------------------ 171184582 Thermoproteus neutrophilus V24Sta archaea>crenarchaeota PAE0886_Pyae_18312250 NPRLLEMRFREREAV----CEPATVPFAVRLDGVGFGK---RLKDFPPP---RSRLVHNALVEVAKSLALT--Q--GADYVHVVSDEINLLFFR-----------------AAPYGGRTFKIISVLASQASAELTAKLGR-----------------------------------------------PLYFDGRVIKLRD--NCDAASYVLFRA--RV--GLNNYVIQLA-----RGAGLIREYTPPIEDMLKSVVIEDYELAWGTFMRRED-G-FKKGVDMCS-------------------------------------------------------------------------------------------------aLSRLCNVC------------------------------ 18312250 Pyrobaculum aerophilum str. IM2 archaea>crenarchaeota Pcal_0070_Pcal_126458698 NPKALEASYREREAV----CEPAAPPFAVRLDGVGFGK---RLRDFPHP---RSRVVHEALVDTAKTLAAT--Y--GAELVHVVSDEINLIFL-----------------GQAPYGGRTFKIVSVLAAHAASELTARLAR-----------------------------------------------PLHFDGRVIRLRD--RCDAATYVLYRA--RV--GLNNYVVQLA-----RGAGLINSHTPHIEELLPKVEIADFELAWGSTMKKEG-G-YRREG-LCE-------------------------------------------------------------------------------------------------aLVALCDVCTSGT-------------------------- 126458698 Pyrobaculum calidifontis JCM 11548 archaea>crenarchaeota Pisl_1144_Pisl_119872651 NPRLLEMRYREREAV----CEPSSPPIAVRLDGVGFGK---RLKDFPAP---RSKLVHAALVEVAKNLAMQ--H--GASFVHVVSDEINLVFL-----------------NVVPYGGRTFKIISVLAAQAAAELTAKLGR-----------------------------------------------PLYFDGRVIKLDS--SCDAAKYILYRA--RV--GLNNYVVQLA-----RATGLIKTQTPHIEELLPKVEIGDFELAWGSFMSKED-G-YLKNSDLCR-------------------------------------------------------------------------------------------------aVSTLCEIC------------------------------ 119872651 Pyrobaculum islandicum DSM 4184 archaea>crenarchaeota Achl_4028_Achl_219882632 --DQTTTDMKNREKQYRTFL-PAKSYAVIRVDGKGFSK-YTR--GLQRP---FDPKFTADMQATALYLCEN--I-DGAQFAYTQSDEISVIISDLGSANT-----------QAWFGGQVQKIVSTSAALATAKFNRIRPEIDA--------------------------------------------LAFFDGRTHHLEG--SAGVLEYLQWRQ-ADA--MKNSVGMLA------SHHFSHRELT----GVSVRRRKEMLAGR-GILWEDLG-QEVKQGTFVRR----------------------------------------------------------------------------------------------vlt-ERSISYL------------HK---KEQVMKTSD----- 219882632 Arthrobacter chlorophenolicus A6 bacteria>actinobacteria CORMATOL_02529_Cmat_225022489 --MNLDTRMKKYEYVTRTYLTCRMP-VIVRLDGKAFHT-FTR--GLKKP---FDSVFNSAMDDTMLYLAQN--S-QNCMLAYRQSDEISLLLVDYATFE-----------TAAWFDNNISKIVSITASMATAVFNESFKKHALAQLESETDQKYCNALRRCIDN-----------------------LALFDSRAFNIPR--E-EVANCFWWRQ-KDA--IKNSIASLG------QAHFSPRELH----GKHGQQIQEM-LETKGISWEDAP-TPQKRGACALR-----------------------------------------------------------------------------------------ndegqwyv-DHNIPVFT-----------E----DWNYIDRFVD---- 225022489 Corynebacterium matruchotii ATCC 33806 bacteria>actinobacteria CORMA0001_0334_Cmat_252128517 --MNLDTRMKKYEYVTRTYLTCRMP-VIVRLDGKAFHT-FTR--GLKKP---FDPVFNSAMDDTMLYLAQN--S-QNCMLAYRQSDEISLLLVDYATFE-----------TAAWFDNNISKIVSITASMATAVFNESFKKHALAQLETETDQKYCNALRRCIDN-----------------------LALFDSRAFNIPR--E-EVANCFWWRQ-KDA--IKNSIASLG------QAHFSPRELH----GKHGQQIQEM-LETKGISWEDAP-TPQKRGACALR-----------------------------------------------------------------------------------------ddegqwhv-DHNIPVFT-----------E----DWNYIDRFVD---- 252128517 Corynebacterium matruchotii ATCC 14266 bacteria>actinobacteria DDB_G0279743_Ddis_66814690 FSALGDRMKSYEDGMKITIEKNN--SFIIRLDGHSFSK-FSK--VFKKPGIAWDIRIHQAMVETATALMKT--F--LPTVVYTFSDEITMCFPSIDKEAIDDG-EIP----QLAYNGKVQKLISLTAGLASTVFYKSITQALYDTDKEEKIIDLLKTA-----------------------------TPSFDSRLFVLPS--NDEIRHNLIWRSIIDC--KRNSVSQVG------QSHFLPKQIH----GLSGQEIKKKLLLEKGIDFNDEP-DWYKYGVYLKK----------------------------------------------------------------------qnythkgfspikpt-qeitvlr------NKVVPFSF-----------DIT--KLSNSNDFITK--- 66814690 Dictyostelium discoideum AX4 eukaryota>amoebozoa>mycetozoa>dictyosteliida ERE_34100_Erec_291529569 DLETRMKTYENVPKNKLMRRCP----VAIRLDGCHFKS-FTK--GFDKP---FDNVFMKSMQETMKYLCEN--V-QGCVMGYTQSDEITLILVDYEHLN-----------SEAWFDDEVEKICSVTAGMASMAFYRIFCKNAHGFLSENGYPHVHMLKNEKLINAYRRAWRQ---------------GAYFDARCFNIPK--E-EVTNLIYWRQ-CCG--HRNAIQAAG------CTYFSNKELL----NKSGDEIIKM-LKEKGIEWSAYS-NDAVWGSCCVR--------------------------------------------------------------------nnrpvimpdgrrtyy--mydknkrtkwii-DYHIPVFTG----------A----GRLYINALVY---- 291529569 Eubacterium rectale M104/1 bacteria>firmicutes nfa39460_Nfar_54025916 --MNLGDRIKAYEAASNYRLTP-NSCVFLRVDGKAFHT--FTR-GMQRP---FDPALMQTMVDAAVETARE--M-QGFKLGYVQSDEATFLLTDFDTHD-----------TAGWFGYEVNKLVSISASTMTMHFNRLFREKP---------------------------------------------MAVFDSRAFVVPR--H-DAPNAFVWRQ-QDW--ARNSLQMLA------RAHFSHRELH----GKGRAELHEM-LMERGVNWAALS-AREKNGTFVLA--------------------------------------------------------------------------------------dksvisekw---GYE----------------DID----VYLTGLMP---- 54025916 Nocardia farcinica IFM 10152 bacteria>actinobacteria RBTH_06728_Bthu_75758411 -MDSIGDRMKRYENAYR-IKLPERMPVIVRIDGAHFHT-YTK--GCAKP---FDQDLAEAFWETCKYLAQN--I-MGAKLVYHQSDEISILITNYDKLT-----------TQSWFENNLQKIASVSASMATAKFNEVMREKYPDKP-----------------------------------------LATFDGRAQVLPQ--D-EVANYFIWRQ-QDA--SKNSISMVA------QANFPHKQLQ----GLNGKDMQDKLMTEKNINWNDLP-VWQKRGICIIK--------------------------------------------------------------------------------efy--ekngalrsrwsv-DHETPIISK----------D-----REYVEQFVY---- 75758411 Bacillus thuringiensis serovar israelensis ATCC 35646 bacteria>firmicutes DDB_G0275625_Ddis_66819813 ELLGDRMKSYEDDMKIQ--IEK-NKPFIIRLDGHSFSK-FTK--NFNKP---HDIRIHNAMIETSTVLLKT--F--MPTCIYTFSDEITMCFPSIDESTLEEGKEIP----NLAYSGKVQKLISLSSGLASTVFFKSITNAQYDKDTELNLIKLLETC-----------------------------TPHFDARIFTLPS--NQEIVNNLIWRSLVDC--KRNSVANLG------FAHFTPKQML----GLNNTEVKKKLLEEKSIIYENEP-AWYRFGTYLKK----------------------------------------------------------------------eyytlttvspnepe-kqitair------SKVNCFSF-----------DITK-LSNSLNFISF---- 66819813 Dictyostelium discoideum AX4 eukaryota>amoebozoa>mycetozoa>dictyosteliida Sterm_2087_Ster_269120695 VHDDFGKRMKTYENSYR-FTLPRRMPVILRIDGCHFHT-FTK--GMDKP---FDDKLIEAFWETCKFLGEN--I-MGAKLIYHQSDEISILITNYDTIQ-----------TDSWFSNNLQKMASVSASMAAAKFNEVIRKSYSDKE-----------------------------------------LAFFDSRAWVIPQ--D-EVNNYFTWRQ-QDA--SKNSISMAA------FANFAHKDLH----GLSGNQLQEKLFSEKGINWDKFP-TWKKRGACIIK--------------------------------------------------------------------------------key--lkenairrrwet-DLDIPLFSK----------D-----RNYVERFVY---- 269120695 Sebaldella termitidis ATCC 33386 bacteria>fusobacteria PPSIR1_23574_Ppac_149920560 ---ALGDRMKLYERAEAGRRFMPLLPICARIDGKRFSR-WTK--GLARP---YDERLSATMVAVTQALVED----THARVGYTQSDEISLVFYSESADS------------QVFLDGRVQKLTSILASMATARFNTEARARVPERADA---------------------------------------PALFDCRCWTVPN--LDEAANALLWRE-RDA--TKNSLSMAA------RSHYSHAQLD----GKRGPDMHEMLHAA-GVNWNDYP-SFFKRGTFVRR--------------------------------------------------------------ervsrpfsteelerlpprhaaranpdlviertvvr-AVDMPPLNRI---------RNR--VAALFEGAE----- 149920560 Plesiocystis pacifica SIR-1 bacteria>proteobacteria>deltaproteobacteria 201phi2-1p319_BP201phi2-1_189490470 --DQLGDRMKMFENDFIVKRFMPGLPIVARIDGRGFSR-FTR--GMKRP---YDPDMSAAMIHTTRELVKH----TQATVGYTQSDEITLIWYSNDYKS------------MNWFDGRVQKMVSLLGSHATLYFNQYIMQYMPQYAKR---------------------------------------NPTFDARVWNVPS--LEEAANVLVWRE-WDA--TKNSIQMAG------HHYFSNKELH----KKNTSEIQEMLWSQHDVNWHHYP-VFFKRGTYIGW-----------------------------------------------------------kqfragrrltdeemlllppkhhahkndgwieketrvlt-ELSLPPIT-----------QIEN-RVEFFFGAKT---- 189490470 Pseudomonas phage 201phi2-1 dsDNA viruses, no RNA stage>caudovirales Igni_0485_Ihos_156937279 ---------MRVEG-------P----VIVRMDGVSFGK-YSK--LLGKH---RDERLHNALVSSAKELVEY--Y--SCDSAYVSSDEVSVYCK------------------LPPFGGRVEKLVSVFSSFLGSHFSVKVSPLP---------------------------------------------PGWFDGRVVLAGD-----WKAYVMWRL--KV--TVCNY----------ASSVARKPCSQALKEVRLPPEA------FGTLLERVE-VLKKGYTR------------------------------------------------------------------------------------------------------------------------------------------- 156937279 Ignicoccus hospitalis KIN4/I archaea>crenarchaeota MIMI_R259_APMV_55819137 INEPIGDRMKRFEAKYDFKIEP-QNYFCVRLDGNKFSN-FTR--KFEKP---YDVRFSQAMVMTTIDTINK--F--GARTGFTQSDEITLIFDKAIPDDFKKHITY-----NHLFNGRVSKLLSIVSSYVSVRFNHHFRLLTSNLTNIYSQETLELINGG---------------------------TAIFDARILEFDENNKYEMLNHLIWRSVKDC--YRNAVQTYA------HHIFGPAKIK----YLNREQMIQLIEENTNIVWSNIP-LWQKYGVIIKK----------------------------------------------------------------------qliktdnvkn--------svit------SSFKVFSL-----------KLSY-NDTMLKFLFD---- 55819137 Acanthamoeba polyphaga mimivirus dsDNA viruses, no RNA stage bthur0002_61310_Bthu_228982997 SSNKLGARMKEYENKTKLTKRSP---VIIRIDGTHFHT-YTK--NMKKP---FDEVLAKAFWETAKYLAQN--I-MGCKMVYHQSDEISLLLTNYEKVT-----------TQSWFGNDLQKMVSVSASMATAKFNEIMFPITGT-------------------------------------------LAFFDSRAFVLPK--E-EVTNYFLWRQ-QDA--TKNSIAMVA------QANFKHKELQ----GYNGSQLQEKLFTEKNINWNNLP-IWQKRGVCITK--------------------------------------------------------------------------------sqy--lkgtatrtkwdv-DFNTPQFS-----------KD----RDYIDQHVF---- 228982997 Bacillus thuringiensis Bt407 bacteria>firmicutes NECHADRAFT_87153_Nhae_256722828 ----LASRMKEYEAITEIHLDP-SKPAILRLDGHSFSK-FTA--SFAKP---FDERLHTAMVKTCADLLGA--Y-SSASLAYTQSDEITLVFPDGV---------------GSQFNGRVAKIASLAAGRCSVHFYSHLVAAVLETPEPPVRGFSSVPFPHFDQQR----------------------LPHFDGRLFNVPS--VEECLSNVIWRCRGDA--IRNSVSGFA------RSLFTTEELH----GKNKEDMLEMVKKK-GFPYEQSVPNWALEGSMVKR----------------------------------------------------------------------tlvrmaavdqktg--etvevvrtrtr--CKDRGITEFS---------DEN---LALVRD------- 256722828 Nectria haematococca mpVI 77-13-4 eukaryota>fungi>ascomycota SNOG_06471_Pnod_169606842 PNIPLAERMKKYEAVYDTTLPS-NSPIILRLDGHNFSR-FTS--HFARP---FDERIHSAMLSTCTSLLTF--F-PSATLAYTQSDEITLIFPSG----------------VGAFGERVQKLSSLAASYTSVNFVKHLIAAVDAQPEPALKGEGGKDVLW---------------------------TAHFDARIFAVPS--IEEALNNLLWRCRNDA--VRNSVSSFA------RTMYSTKEMH----GKRAKELVAMMREEKGVVFEDAVPKWAIEGCLIKR----------------------------------------------------------------------eqvdhwgrngktg--ememtfrarvr--VEERGVREFG---------EEG---LRLVQE------- 169606842 Phaeosphaeria nodorum SN15 eukaryota>fungi>ascomycota PTRG_02487_Ptri_189192963 TTQSLASRMKSYESTFDHTLPL-TSPIILRLDGHGFSR-FTA--HFARP---FDQRIHLAMTRTSSDLLSY--F-PSATLAYTQSDEITLVFPSG----------------VQTFNSRVQKLSSIAASYCSVRFNKHLSAALRELTEPRVSGDVEEWLG----------------------------TAHFDARFFPVPN--VEEALNNLIWRCRNDA--VRNAVSGFA------RTMYTTAEMH----GKKTNELIEMMLQDKGVRFEEAVPKWAIEGCLIKR----------------------------------------------------------------------eqyeheglnmktg--ekektfrtrtr--VEERGVREFN---------DEG---LRLITD------- 189192963 Pyrenophora tritici-repentis Pt-1C-BFP eukaryota>fungi>ascomycota PPL_08738_Ppal_281203894 DSVTLGDRMKQYEVSMNTLHITDNTPFIIRLDGHGFSK-FTK--NFVKP---WDIRVHNAMVETATVLMKE--F--NPTLVYTFSDEITLCFSSLPDQEYQERLIATQSQSLLPYNGKVQKLITLAAGIASTTFYKVITSQTYDSATEPKLTQYLAES-----------------------------LPHFDARIFTLPD--NQEIINNLVWRSVIDC--KRNSISGLA------QAHFPHKQIQ----GKGGKEMK-SMLLAKGIDYYKEP-MWYRFGVFLKK----------------------------------------------------------------------qyytldsvspvnn--qsvqsirskir--RDSFNIQHF----------PNA---LDYLTI------- 281203894 Polysphondylium pallidum PN500 eukaryota>amoebozoa>mycetozoa>dictyosteliida TTHERM_00373760_Tthe_118352666 SKKSIGDRMKEYELSARSKIDP-SLPAIMRIDGHSFSK-FTK--GLKKP---YDEWLHKLMVETTAALISE--F--SFNIGYTQSDEITLVYLPSFDKSGKLN--------DYPYANQIMKLVSLSSAFATNYFTRGIQRGIQDKTINLEDYQESTITKLQNP------------------------KCYFDSRIFNVPS--IQEVYSNIYWRSCYDC--IKNSVSMVA------YVHFPVKLTD----GLHTQAKIKKLLEEKNIDWNKDFSNHFKYGTFIKK----------------------------------------------------------------rqfeidlpleyqkfkkdss--nqkvnrt------ETVAFSYNFSGNKFN----EDT---KNILFE------- 118352666 Tetrahymena thermophila eukaryota>alveolata>ciliophora MA0817_Mace_20089701 ---------KTREIYAEMRCIP---PVVLRADGRNFKN-TLSGLGFEKP---YDKTFARAMADTAELFIKKSGL--SPLFAYTFSDEISFLFT------------------DLPFDGRVEKIDSVVASFLGSALTIKLRLEE---------------------------------------------PIAFDSRLVALQK---EEIPEYFHRRQ-LEA--WRNFVAS------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 20089701 Methanosarcina acetivorans C2A archaea>euryarchaeota Cyan7822DRAFT_2032_Csp._196256337 KFEELDAKLRVFETGHDFCVLP-EIFMIARLDGRNFTRLTKDTHQFEAP---FDLKFRDYMVATVEHLMNC-GF--RVVYGYTQSDEISLLLHRDE---------------TT-FGRKLRKLNSVLAGEASAKLSLLLGT-----------------------------------------------LAAFDCRISQLPT--LNLVVDYFRI---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 196256337 Cyanothece sp. PCC 7822 bacteria>cyanobacteria COPEUT_02780_Ceut_163816590 -------MKDFYEQIPKTKLMR-RCPVAIRIDGKAFHT-YTR--NFKRP---FDSVFMRAMQETMKYLCEN--IQ-GCVLGYTQSDEITLILVDYEKIN-----------SSAWFDYEVQKLCSVSASMATMRFNQVFARLGR--------------------------------------------CLIQDASIYRRKR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 163816590 Coprococcus eutactus ATCC 27759 bacteria>firmicutes NAEGRDRAFT_61112_Ngru_284097001 ------LFEGQYDSKVNFDIAD-QQPIVMRFDGFHFKS-FTTNGDIFEKP--FDANIHWALLLAVRDLCLK-NLSMNPTLIYNCSDELTLLFSGQTSNNEKQKV------PNGIYRGRAQKLVSISTSFLTITFNYYLRKLLRANADSFESRLEYDMHNSNISKKSYQTEDFEKRRLVRQKLLDGDIFGNFDCRIYQLPT--ISHAIDYMIWRQ-IDCIRNSKTTLGSKFYA---QNEMNFLSAS-KIVEMVEQEKVHLLNMI--IPLRIDG-DVLDRAIRLV----------------------------------------------------------------------------------------------------------------------------------------- 284097001 Naegleria gruberi eukaryota>heterolobosea NEMVEDRAFT_v1g147966_Nvec_156340600 --------MRKNETLFDQHVLP-DNYIVVRLDGKGFTK-LTKESLDLEKP--FDIRFHDAMVATTKHLLTV-GF--KVIYAYTQSDEISLLIDK----------------DDNTFNRKVRKINSVLAGEASAFFSMYFNK-----------------------------------------------LSVLDCRTICIPN--IEMLLDYFCWRQ-EDA--HRNSLSAYCYWTLR-NNGNSYIEATKKTEKLSVSDKNELLFQH-GINYNSVP-SWQKRGVGMFYesidrkgin------------------------------------------------------------------------------------------------------------------------------- 156340600 Nematostella vectensis eukaryota>metazoa>cnidaria consensus/90% .......b.b.......--h..-...hllRlDGp.Fpp.hsp...h.+P....D..h.phMs.ss..lh.p..h.....huas.SDEhohhh......................h..p..Kh.o..su.hss.a...h..................................................s.FDuRhh.hsp...p.l.sYh.WRp..-s....Nsb.s.s...h.....bs..ph.....s....pbp-hlbpp.slshsp.s...b+bG..h.b.....................................................................................................................p.......h.......... Mutated Residues with effect ............@................*.................@............................#.....$..**..............................*..*................$.....................................................*.*..............$...*...#...*$*..*@............................*.@........@..........@... The Mutated Residues marked are from the paper Jackman JE, Phizicky EM: Identification of critical residues for G-1 addition and substrate recognition by tRNA(His) guanylyltransferase. Biochemistry 2008, 47(16):4817-4825. * (R27, D77, E78, R93, K96, D131, R133, R150, H155, N157, Y160, and K190),corresponding variants have defects in both G-1 addition and complementation # (D153A) causes a significant growth defect that does not correlate with decreased G-1 addition activity, and the high level of activity measured with this variant. @ (E13, K44, N161, E192, N201, and K211) the alanine variants have substantially reduced activity in vitro, yet there is no identifiable phenotype of the corresponding yeast thg1 mutant strains. $ Alterations at (G74, W113/I156, and Y146) result in a temperature-sensitive growth of the thg1 mutant strains. # a single variant (D68A) demonstrated dramatically altered substrate specificity compared to the wild-type enzyme, which is rigorously specific for tRNAHis. |
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Family clusters of Thg1 domain containing proteins with domain architectures
# 223; Thg1 solos
82617278 C4_0009 262 uncultured archaeon archaea conserved hypothetical protein [uncultured archaeon].
268323173 BSM_02380 253 uncultured archaeon archaea conserved hypothetical protein containing tRNAHis
126458698 Pcal_0070 225 Pyrobaculum calidifontis JCM 11548 archaea>crenarchaeota hypothetical protein Pcal_0070 [Pyrobaculum calidifontis JCM
145590370 Pars_0105 196 Pyrobaculum arsenaticum DSM 13514 archaea>crenarchaeota hypothetical protein Pars_0105 [Pyrobaculum arsenaticum DSM 13514].
156937279 Igni_0485 162 Ignicoccus hospitalis KIN4/I archaea>crenarchaeota hypothetical protein Igni_0485 [Ignicoccus hospitalis KIN4/I].
18312250 PAE0886 212 Pyrobaculum aerophilum str. IM2 archaea>crenarchaeota hypothetical protein PAE0886 [Pyrobaculum aerophilum str. IM2].
119872651 Pisl_1144 212 Pyrobaculum islandicum DSM 4184 archaea>crenarchaeota hypothetical protein Pisl_1144 [Pyrobaculum islandicum DSM 4184].
124027917 Hbut_1048 238 Hyperthermus butylicus DSM 5456 archaea>crenarchaeota hypothetical protein Hbut_1048 [Hyperthermus butylicus DSM 5456].
171184582 Tneu_0097 212 Thermoproteus neutrophilus V24Sta archaea>crenarchaeota hypothetical protein Tneu_0097 [Thermoproteus neutrophilus V24Sta].
20093533 MK0093 262 Methanopyrus kandleri AV19 archaea>euryarchaeota hypothetical protein MK0093 [Methanopyrus kandleri AV19].
288559933 thgL 252 Methanobrevibacter ruminantium M1 archaea>euryarchaeota tRNA(His) guanylyltransferase ThgL [Methanobrevibacter ruminantium
282165226 MCP_2556 247 Methanocella paludicola SANAE archaea>euryarchaeota hypothetical protein MCP_2556 [Methanocella paludicola SANAE].
116754185 Mthe_0875 235 Methanosaeta thermophila PT archaea>euryarchaeota hypothetical protein Mthe_0875 [Methanosaeta thermophila PT].
84489939 Msp_1147 237 Methanosphaera stadtmanae DSM 3091 archaea>euryarchaeota hypothetical protein Msp_1147 [Methanosphaera stadtmanae DSM 3091].
15678990 MTH972 246 Methanothermobacter thermautotrophicus str. Delta H archaea>euryarchaeota hypothetical protein MTH972 [Methanothermobacter thermautotrophicus
20089701 MA0817 141 Methanosarcina acetivorans C2A archaea>euryarchaeota hypothetical protein MA0817 [Methanosarcina acetivorans C2A].
154151809 Mboo_2270 250 Candidatus Methanoregula boonei 6A8 archaea>euryarchaeota hypothetical protein Mboo_2270 [Candidatus Methanoregula boonei
261349483 METSMIF1_02117 239 Methanobrevibacter smithii DSM 2374 archaea>euryarchaeota tRNAHis guanylyltransferase family protein [Methanobrevibacter
222445979 METSMIALI_01627 239 Methanobrevibacter smithii DSM 2375 archaea>euryarchaeota hypothetical protein METSMIALI_01627 [Methanobrevibacter smithii
148642523 Msm_0463 239 Methanobrevibacter smithii ATCC 35061 archaea>euryarchaeota tRNA(His) guanylyltransferase [Methanobrevibacter smithii ATCC
124485581 Mlab_0759 240 Methanocorpusculum labreanum Z archaea>euryarchaeota hypothetical protein Mlab_0759 [Methanocorpusculum labreanum Z].
88601907 Mhun_0610 241 Methanospirillum hungatei JF-1 archaea>euryarchaeota hypothetical protein Mhun_0610 [Methanospirillum hungatei JF-1].
284162827 Arcpr_1732 246 Archaeoglobus profundus DSM 5631 archaea>euryarchaeota protein of unknown function DUF549 [Archaeoglobus profundus DSM
147920979 RCIX455 241 uncultured methanogenic archaeon RC-I archaea>euryarchaeota hypothetical protein RCIX455 [uncultured methanogenic archaeon
91773429 Mbur_1464 243 Methanococcoides burtonii DSM 6242 archaea>euryarchaeota hypothetical protein Mbur_1464 [Methanococcoides burtonii DSM
73669253 Mbar_A1746 243 Methanosarcina barkeri str. Fusaro archaea>euryarchaeota hypothetical protein Mbar_A1746 [Methanosarcina barkeri str.
294496410 Mmah_1763 243 Methanohalophilus mahii DSM 5219 archaea>euryarchaeota tRNA(His)-5'-guanylyltransferase [Methanohalophilus mahii DSM
219850835 Mpal_0151 243 Methanosphaerula palustris E1-9c archaea>euryarchaeota protein of unknown function DUF549 [Candidatus Methanosphaerula
21228066 MM_1964 243 Methanosarcina mazei Go1 archaea>euryarchaeota hypothetical protein MM_1964 [Methanosarcina mazei Go1].
126178352 Memar_0402 244 Methanoculleus marisnigri JR1 archaea>euryarchaeota hypothetical protein Memar_0402 [Methanoculleus marisnigri JR1].
170290817 Kcr_1204 249 Candidatus Korarchaeum cryptofilum OPF8 archaea>korarchaeota hypothetical protein Kcr_1204 [Candidatus Korarchaeum cryptofilum
196256337 Cyan7822DRAFT_2032 141 Cyanothece sp. PCC 7822 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Cyanothece sp. PCC
171913885 VspiD_010100021940 159 Verrucomicrobium spinosum DSM 4136 bacteria>chlamydiae/verrucomicrobia tRNAHis guanylyltransferase family protein [Verrucomicrobium
153872919 BGP_4908 214 Beggiatoa sp. PS bacteria>proteobacteria>gammaproteobacteria protein of unknown function DUF549 [Beggiatoa sp. PS].
54025916 nfa39460 229 Nocardia farcinica IFM 10152 bacteria>actinobacteria hypothetical protein nfa39460 [Nocardia farcinica IFM 10152].
219882632 Achl_4028 238 Arthrobacter chlorophenolicus A6 bacteria>actinobacteria hypothetical protein Achl_4028 [Arthrobacter chlorophenolicus A6].
269120695 Sterm_2087 241 Sebaldella termitidis ATCC 33386 bacteria>fusobacteria hypothetical protein Sterm_2087 [Sebaldella termitidis ATCC 33386].
75758411 RBTH_06728 245 Bacillus thuringiensis serovar israelensis ATCC 35646 bacteria>firmicutes Hypothetical protein RBTH_06728 [Bacillus thuringiensis serovar
162454339 sce6062 248 Sorangium cellulosum 'So ce 56' bacteria>proteobacteria>deltaproteobacteria hypothetical protein sce6062 [Sorangium cellulosum 'So ce 56'].
124008957 M23134_06676 250 Microscilla marina ATCC 23134 bacteria>bacteroidetes/chlorobi conserved protein [Microscilla marina ATCC 23134].
252128517 CORMA0001_0334 250 Corynebacterium matruchotii ATCC 14266 bacteria>actinobacteria trnahis guanylyltransferase family protein [Corynebacterium
225022489 CORMATOL_02529 250 Corynebacterium matruchotii ATCC 33806 bacteria>actinobacteria hypothetical protein CORMATOL_02529 [Corynebacterium matruchotii
255067726 NEISICOT_02755 251 Neisseria sicca ATCC 29256 bacteria>proteobacteria>betaproteobacteria tRNA(His) guanylyltransferase family protein [Neisseria sicca ATCC
225075026 NEIFLAOT_00025 252 Neisseria flavescens NRL30031/H210 bacteria>proteobacteria>betaproteobacteria hypothetical protein NEIFLAOT_00025 [Neisseria flavescens
159900562 Haur_4049 252 Herpetosiphon aurantiacus ATCC 23779 bacteria>chloroflexi hypothetical protein Haur_4049 [Herpetosiphon aurantiacus ATCC
158336895 AM1_3767 253 Acaryochloris marina MBIC11017 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Acaryochloris marina
239627528 CBFG_01250 253 Clostridiales bacterium 1_7_47FAA bacteria>firmicutes tRNAHis guanylyltransferase family protein [Clostridiales bacterium
294671196 NEIELOOT_02904 255 Neisseria elongata subsp. glycolytica ATCC 29315 bacteria>proteobacteria>betaproteobacteria hypothetical protein NEIELOOT_02904 [Neisseria elongata subsp.
256424765 Cpin_5797 255 Chitinophaga pinensis DSM 2588 bacteria>bacteroidetes/chlorobi tRNA(His)-5'-guanylyltransferase [Chitinophaga pinensis DSM 2588].
254412407 MC7420_6362 255 Microcoleus chthonoplastes PCC 7420 bacteria>cyanobacteria tRNAHis guanylyltransferase superfamily [Microcoleus chthonoplastes
258545199 HMPREF0198_1468 256 Cardiobacterium hominis ATCC 15826 bacteria>proteobacteria>gammaproteobacteria tRNAHis guanylyltransferase family protein [Cardiobacterium hominis
229539832 PlimDRAFT_36970 258 Planctomyces limnophilus DSM 3776 bacteria>planctomycetes tRNA(His)-5'-guanylyltransferase [Planctomyces limnophilus DSM
108762077 MXAN_5968 267 Myxococcus xanthus DK 1622 bacteria>proteobacteria>deltaproteobacteria tRNAHis guanylyltransferase family protein [Myxococcus xanthus DK
149920560 PPSIR1_23574 274 Plesiocystis pacifica SIR-1 bacteria>proteobacteria>deltaproteobacteria hypothetical protein PPSIR1_23574 [Plesiocystis pacifica SIR-1].
168698293 GobsU_010100002157 279 Gemmata obscuriglobus UQM 2246 bacteria>planctomycetes hypothetical protein GobsU_02157 [Gemmata obscuriglobus UQM 2246].
291529569 ERE_34100 285 Eubacterium rectale M104/1 bacteria>firmicutes Uncharacterized conserved protein [Eubacterium rectale M104/1].
228982997 bthur0002_61310 353 Bacillus thuringiensis Bt407 bacteria>firmicutes hypothetical protein bthur0002_61310 [Bacillus thuringiensis
55819137 MIMI_R259 292 Acanthamoeba polyphaga mimivirus dsDNA viruses, no RNA stage hypothetical protein MIMI_R259 [Acanthamoeba polyphaga mimivirus].
189490470 201phi2-1p319 270 Pseudomonas phage 201phi2-1 dsDNA viruses, no RNA stage>caudovirales putative Thg1 [Pseudomonas phage 201phi2-1].
221052852 PKH_031670 282 Plasmodium knowlesi strain H eukaryota>alveolata>apicomplexa tRNA guanylyltransferase [Plasmodium knowlesi strain H].
68070617 PB000744.00.0 148 Plasmodium berghei str. ANKA eukaryota>alveolata>apicomplexa hypothetical protein [Plasmodium berghei strain ANKA].
209557756 CMU_028750 288 Cryptosporidium muris RN66 eukaryota>alveolata>apicomplexa tRNA(His) guanylyltransferase, putative [Cryptosporidium muris
156093512 PVX_096205 364 Plasmodium vivax SaI-1 eukaryota>alveolata>apicomplexa hypothetical protein [Plasmodium vivax SaI-1].
83285854 PY02052 356 Plasmodium yoelii yoelii str. 17XNL eukaryota>alveolata>apicomplexa hypothetical protein [Plasmodium yoelii yoelii str. 17XNL].
124511996 PF07_0095 299 Plasmodium falciparum 3D7 eukaryota>alveolata>apicomplexa hypothetical protein [Plasmodium falciparum 3D7].
84999512 TA19625 330 Theileria annulata strain Ankara eukaryota>alveolata>apicomplexa hypothetical protein [Theileria annulata].
156086032 BBOV_IV004960 324 Babesia bovis T2Bo eukaryota>alveolata>apicomplexa tRNA-His guanylyltransferase [Babesia bovis T2Bo].
71032267 TP01_0248 293 Theileria parva strain Muguga eukaryota>alveolata>apicomplexa hypothetical protein [Theileria parva strain Muguga].
126643977 cgd1_1990 296 Cryptosporidium parvum Iowa II eukaryota>alveolata>apicomplexa hypothetical protein [Cryptosporidium parvum Iowa II].
118358858 TTHERM_00085230 334 Tetrahymena thermophila eukaryota>alveolata>ciliophora tRNAHis guanylyltransferase family protein [Tetrahymena
118352666 TTHERM_00373760 322 Tetrahymena thermophila eukaryota>alveolata>ciliophora hypothetical protein TTHERM_00373760 [Tetrahymena thermophila].
145533455 GSPATT00002474001 290 Paramecium tetraurelia strain d4-2 eukaryota>alveolata>ciliophora hypothetical protein [Paramecium tetraurelia strain d4-2].
66814690 DDB_G0279743 415 Dictyostelium discoideum AX4 eukaryota>amoebozoa>mycetozoa>dictyosteliida hypothetical protein DDB_G0279743 [Dictyostelium discoideum AX4].
66819813 DDB_G0275625 291 Dictyostelium discoideum AX4 eukaryota>amoebozoa>mycetozoa>dictyosteliida hypothetical protein DDB_G0275625 [Dictyostelium discoideum AX4].
66805193 DDB_G0289191 278 Dictyostelium discoideum AX4 eukaryota>amoebozoa>mycetozoa>dictyosteliida hypothetical protein DDB_G0289191 [Dictyostelium discoideum AX4].
281203894 PPL_08738 308 Polysphondylium pallidum PN500 eukaryota>amoebozoa>mycetozoa>dictyosteliida hypothetical protein PPL_08738 [Polysphondylium pallidum PN500].
66802352 thg1 256 Dictyostelium discoideum AX4 eukaryota>amoebozoa>mycetozoa>dictyosteliida tRNA-histidine guanylyltransferase 1 [Dictyostelium discoideum
167518403 MONBRDRAFT_31372 330 Monosiga brevicollis MX1 eukaryota>choanoflagellida hypothetical protein [Monosiga brevicollis MX1].
119186387 CIMG_03241 287 Coccidioides immitis RS eukaryota>fungi>ascomycota hypothetical protein CIMG_03241 [Coccidioides immitis RS].
189197179 PTRG_04594 207 Pyrenophora tritici-repentis Pt-1C-BFP eukaryota>fungi>ascomycota histidyl tRNA-specific guanylyltransferase [Pyrenophora
225677608 PABG_05979 335 Paracoccidioides brasiliensis Pb03 eukaryota>fungi>ascomycota conserved hypothetical protein [Paracoccidioides brasiliensis
119494994 NFIA_010790 291 Neosartorya fischeri NRRL 181 eukaryota>fungi>ascomycota tRNAHis guanylyltransferase, putative [Neosartorya fischeri NRRL
145230810 An01g12630 281 Aspergillus niger CBS 513.88 eukaryota>fungi>ascomycota hypothetical protein An01g12630 [Aspergillus niger].
256722828 NECHADRAFT_87153 276 Nectria haematococca mpVI 77-13-4 eukaryota>fungi>ascomycota hypothetical protein NECHADRAFT_87153 [Nectria haematococca mpVI
255941868 Pc16g14050 275 Penicillium chrysogenum Wisconsin 54-1255 eukaryota>fungi>ascomycota Pc16g14050 [Penicillium chrysogenum Wisconsin 54-1255].
226295250 PADG_06749 291 Paracoccidioides brasiliensis Pb18 eukaryota>fungi>ascomycota tRNA(His) guanylyltransferase [Paracoccidioides brasiliensis Pb18].
149234768 LELG_05489 273 Lodderomyces elongisporus NRRL YB-4239 eukaryota>fungi>ascomycota hypothetical protein LELG_05489 [Lodderomyces elongisporus NRRL
171695070 PODANSg09507 273 Podospora anserina DSM 980 eukaryota>fungi>ascomycota unnamed protein product [Podospora anserina].
189192963 PTRG_02487 272 Pyrenophora tritici-repentis Pt-1C-BFP eukaryota>fungi>ascomycota conserved hypothetical protein [Pyrenophora tritici-repentis
156054634 SS1G_06165 226 Sclerotinia sclerotiorum 1980 UF-70 eukaryota>fungi>ascomycota hypothetical protein SS1G_06165 [Sclerotinia sclerotiorum 1980].
254566669 PAS_chr1-4_0323 271 Pichia pastoris GS115 eukaryota>fungi>ascomycota tRNAHis guanylyltransferase [Pichia pastoris GS115].
6321461 THG1 237 Saccharomyces cerevisiae eukaryota>fungi>ascomycota Thg1p [Saccharomyces cerevisiae].
50303737 KLLA0B06237g 237 Kluyveromyces lactis NRRL Y-1140 eukaryota>fungi>ascomycota unnamed protein product [Kluyveromyces lactis].
50290577 CAGL0J00209g 237 Candida glabrata CBS 138 eukaryota>fungi>ascomycota unnamed protein product [Candida glabrata].
45185260 AGOS_ABR031C 237 Ashbya gossypii ATCC 10895 eukaryota>fungi>ascomycota ABR031Cp [Ashbya gossypii ATCC 10895].
259146527 EC1118_1G1_3257g 237 Saccharomyces cerevisiae EC1118 eukaryota>fungi>ascomycota Thg1p [Saccharomyces cerevisiae EC1118].
255718999 KLTH0G17270g 237 Lachancea thermotolerans CBS 6340 eukaryota>fungi>ascomycota KLTH0G17270p [Lachancea thermotolerans].
150865885 PICST_60761 268 Pichia stipitis CBS 6054 eukaryota>fungi>ascomycota hypothetical protein PICST_60761 [Pichia stipitis CBS 6054].
169606842 SNOG_06471 268 Phaeosphaeria nodorum SN15 eukaryota>fungi>ascomycota hypothetical protein SNOG_06471 [Phaeosphaeria nodorum SN15].
238883228 CAWG_05412 268 Candida albicans WO-1 eukaryota>fungi>ascomycota hypothetical protein CAWG_05412 [Candida albicans WO-1].
241957021 CD36_70100 268 Candida dubliniensis CD36 eukaryota>fungi>ascomycota tRNA(His) guanylyltransferase, putative; tRNA-histidine
240274622 HCDG_07873 292 Ajellomyces capsulatus H143 eukaryota>fungi>ascomycota tRNA(His) guanylyltransferase [Ajellomyces capsulatus H143].
46442219 THG1 268 Candida albicans SC5314 eukaryota>fungi>ascomycota likely histidyl tRNA-specific guanylyltransferase [Candida albicans
254577809 ZYRO0A12144g 237 Zygosaccharomyces rouxii CBS 732 eukaryota>fungi>ascomycota ZYRO0A12144p [Zygosaccharomyces rouxii].
260946739 CLUG_03111 267 Clavispora lusitaniae ATCC 42720 eukaryota>fungi>ascomycota hypothetical protein CLUG_03111 [Clavispora lusitaniae ATCC 42720].
289622466 SMAC_05003 267 Sordaria macrospora eukaryota>fungi>ascomycota unnamed protein product [Sordaria macrospora].
261358866 VDBG_07404 288 Verticillium albo-atrum VaMs.102 eukaryota>fungi>ascomycota tRNA(His) guanylyltransferase [Verticillium albo-atrum VaMs.102].
146422179 PGUG_00408 265 Pichia guilliermondii ATCC 6260 eukaryota>fungi>ascomycota hypothetical protein PGUG_00408 [Pichia guilliermondii ATCC 6260].
164429701 NCU02105 265 Neurospora crassa OR74A eukaryota>fungi>ascomycota hypothetical protein NCU02105 [Neurospora crassa OR74A].
190344604 PGUG_00408 265 Pichia guilliermondii ATCC 6260 eukaryota>fungi>ascomycota hypothetical protein PGUG_00408 [Pichia guilliermondii ATCC 6260].
256734706 NECHADRAFT_30739 265 Nectria haematococca mpVI 77-13-4 eukaryota>fungi>ascomycota hypothetical protein NECHADRAFT_30739 [Nectria haematococca mpVI
294659006 DEHA2F22880g 265 Debaryomyces hansenii CBS767 eukaryota>fungi>ascomycota DEHA2F22880p [Debaryomyces hansenii CBS767].
50425487 DEHA0F24321g 265 Debaryomyces hansenii CBS767 eukaryota>fungi>ascomycota hypothetical protein DEHA0F24321g [Debaryomyces hansenii CBS767].
213409163 SJAG_04233 263 Schizosaccharomyces japonicus yFS275 eukaryota>fungi>ascomycota tRNA(His) guanylyltransferase [Schizosaccharomyces japonicus
238837882 MCYG_00432 292 Microsporum canis CBS 113480 eukaryota>fungi>ascomycota histidine tRNA 5'-guanylyltransferase [Microsporum canis CBS
85111778 NCU02105.1 293 Neurospora crassa OR74A eukaryota>fungi>ascomycota hypothetical protein [Neurospora crassa OR74A].
19075481 SPCC63.07 261 Schizosaccharomyces pombe eukaryota>fungi>ascomycota tRNA guanylyltransferase (predicted) [Schizosaccharomyces pombe
156843110 Kpol_526p19 238 Vanderwaltozyma polyspora DSM 70294 eukaryota>fungi>ascomycota hypothetical protein Kpol_526p19 [Vanderwaltozyma polyspora DSM
239612828 BDCG_04935 239 Ajellomyces dermatitidis ER-3 eukaryota>fungi>ascomycota tRNA(His) guanylyltransferase [Ajellomyces dermatitidis ER-3].
255731634 CTRG_05039 268 Candida tropicalis MYA-3404 eukaryota>fungi>ascomycota hypothetical protein CTRG_05039 [Candida tropicalis MYA-3404].
212533511 PMAA_074380 312 Penicillium marneffei ATCC 18224 eukaryota>fungi>ascomycota tRNAHis guanylyltransferase Thg1, putative [Penicillium marneffei
70996154 AFUA_1G14630 372 Aspergillus fumigatus Af293 eukaryota>fungi>ascomycota tRNAHis guanylyltransferase Thg1 [Aspergillus fumigatus Af293].
159131585 AFUB_014160 374 Aspergillus fumigatus A1163 eukaryota>fungi>ascomycota tRNAHis guanylyltransferase Thg1, putative [Aspergillus fumigatus
154320945 BC1G_01347 153 Botryotinia fuckeliana B05.10 eukaryota>fungi>ascomycota hypothetical protein BC1G_01347 [Botryotinia fuckeliana B05.10].
226278784 PAAG_05399 368 Paracoccidioides brasiliensis Pb01 eukaryota>fungi>ascomycota conserved hypothetical protein [Paracoccidioides brasiliensis
242778279 TSTA_094890 290 Talaromyces stipitatus ATCC 10500 eukaryota>fungi>ascomycota tRNAHis guanylyltransferase Thg1, putative [Talaromyces stipitatus
46108064 FG00914.1 253 Gibberella zeae PH-1 eukaryota>fungi>ascomycota hypothetical protein FG00914.1 [Gibberella zeae PH-1].
121701067 ACLA_020800 296 Aspergillus clavatus NRRL 1 eukaryota>fungi>ascomycota tRNAHis guanylyltransferase, putative [Aspergillus clavatus NRRL
67517047 AN0804.2 255 Aspergillus nidulans FGSC A4 eukaryota>fungi>ascomycota hypothetical protein AN0804.2 [Aspergillus nidulans FGSC A4].
169770385 AO090003000484 295 Aspergillus oryzae RIB40 eukaryota>fungi>ascomycota hypothetical protein [Aspergillus oryzae RIB40].
170108182 LACBIDRAFT_252840 256 Laccaria bicolor S238N-H82 eukaryota>fungi>basidiomycota predicted protein [Laccaria bicolor S238N-H82].
242205948 POSPLDRAFT_87675 283 Postia placenta Mad-698-R eukaryota>fungi>basidiomycota predicted protein [Postia placenta Mad-698-R].
58265566 CNC02800 285 Cryptococcus neoformans var. neoformans JEC21 eukaryota>fungi>basidiomycota tRNA guanylyltransferase [Cryptococcus neoformans var. neoformans
242214759 POSPLDRAFT_88866 293 Postia placenta Mad-698-R eukaryota>fungi>basidiomycota predicted protein [Postia placenta Mad-698-R].
169860017 CC1G_06232 353 Coprinopsis cinerea okayama7#130 eukaryota>fungi>basidiomycota hypothetical protein CC1G_06232 [Coprinopsis cinerea okayama7#130].
238663948 Smp_073990 113 Schistosoma mansoni eukaryota>metazoa conserved hypothetical protein [Schistosoma mansoni].
198436739 LOC100179812 279 Ciona intestinalis eukaryota>metazoa PREDICTED: similar to interphase cytoplasmic foci protein 45 [Ciona
241096199 IscW_ISCW001226 258 Ixodes scapularis eukaryota>metazoa conserved hypothetical protein [Ixodes scapularis].
260828313 BRAFLDRAFT_60263 270 Branchiostoma floridae eukaryota>metazoa hypothetical protein BRAFLDRAFT_60263 [Branchiostoma floridae].
221135876 LOC100205752 259 Hydra magnipapillata eukaryota>metazoa>cnidaria PREDICTED: similar to predicted protein [Hydra magnipapillata].
156403638 NEMVEDRAFT_v1g84802 260 Nematostella vectensis eukaryota>metazoa>cnidaria predicted protein [Nematostella vectensis].
156340600 NEMVEDRAFT_v1g147966 207 Nematostella vectensis eukaryota>metazoa>cnidaria hypothetical protein NEMVEDRAFT_v1g147966 [Nematostella vectensis].
225712866 THG1 317 Lepeophtheirus salmonis eukaryota>metazoa>crustacea Probable tRNAHis guanylyltransferase [Lepeophtheirus salmonis].
225719698 THG1 296 Caligus clemensi eukaryota>metazoa>crustacea Probable tRNAHis guanylyltransferase [Caligus clemensi].
225711510 THG1 307 Caligus rogercresseyi eukaryota>metazoa>crustacea Probable tRNAHis guanylyltransferase [Caligus rogercresseyi].
225712230 THG1 300 Lepeophtheirus salmonis eukaryota>metazoa>crustacea Probable tRNAHis guanylyltransferase [Lepeophtheirus salmonis].
225710266 THG1 285 Caligus rogercresseyi eukaryota>metazoa>crustacea Probable tRNAHis guanylyltransferase [Caligus rogercresseyi].
Dpul1000000854 Dpul1000000854 257 Daphnia pulex eukaryota>metazoa>crustacea e_gw1.1.708.1
115921197 LOC587315 265 Strongylocentrotus purpuratus eukaryota>metazoa>echinodermata PREDICTED: hypothetical protein [Strongylocentrotus purpuratus].
291224944 LOC100374565 341 Saccoglossus kowalevskii eukaryota>metazoa>hemichordata PREDICTED: CG4103-like [Saccoglossus kowalevskii].
193673956 LOC100165160 272 Acyrthosiphon pisum eukaryota>metazoa>hexapoda PREDICTED: similar to predicted protein isoform 2 [Acyrthosiphon
195155911 Dper\GL25730 293 Drosophila persimilis eukaryota>metazoa>hexapoda GL25730 [Drosophila persimilis].
195474109 Dyak\GE24732 287 Drosophila yakuba eukaryota>metazoa>hexapoda GE24732 [Drosophila yakuba].
19921364 CG4103 286 Drosophila melanogaster eukaryota>metazoa>hexapoda CG4103 [Drosophila melanogaster].
242016870 Phum_PHUM410370 252 Pediculus humanus corporis eukaryota>metazoa>hexapoda conserved hypothetical protein [Pediculus humanus corporis].
195579310 Dsim\GD21976 287 Drosophila simulans eukaryota>metazoa>hexapoda GD21976 [Drosophila simulans].
114051932 LOC692885 301 Bombyx mori eukaryota>metazoa>hexapoda interphase cyctoplasmic foci protein 45 [Bombyx mori].
289742577 - 306 Glossina morsitans morsitans eukaryota>metazoa>hexapoda uncharacterized conserved protein [Glossina morsitans morsitans].
195338513 Dsec\GM14588 287 Drosophila sechellia eukaryota>metazoa>hexapoda GM14588 [Drosophila sechellia].
158300966 AgaP_AGAP011752 307 Anopheles gambiae str. PEST eukaryota>metazoa>hexapoda AGAP011752-PA [Anopheles gambiae str. PEST].
195030226 Dgri\GH10821 286 Drosophila grimshawi eukaryota>metazoa>hexapoda GH10821 [Drosophila grimshawi].
195437103 Dwil\GK18307 282 Drosophila willistoni eukaryota>metazoa>hexapoda GK18307 [Drosophila willistoni].
239788252 ACYPI006122 272 Acyrthosiphon pisum eukaryota>metazoa>hexapoda ACYPI006122 [Acyrthosiphon pisum].
170028733 CpipJ_CPIJ000684 312 Culex quinquefasciatus eukaryota>metazoa>hexapoda tRNA(His) guanylyltransferase [Culex quinquefasciatus].
157138643 AaeL_AAEL014040 314 Aedes aegypti eukaryota>metazoa>hexapoda hypothetical protein AaeL_AAEL014040 [Aedes aegypti].
156547518 LOC100117826 323 Nasonia vitripennis eukaryota>metazoa>hexapoda PREDICTED: similar to interphase cyctoplasmic foci protein 45
195385629 Dvir\GJ16007 278 Drosophila virilis eukaryota>metazoa>hexapoda GJ16007 [Drosophila virilis].
194857325 Dere\GG24229 287 Drosophila erecta eukaryota>metazoa>hexapoda GG24229 [Drosophila erecta].
91083329 LOC663767 291 Tribolium castaneum eukaryota>metazoa>hexapoda PREDICTED: similar to interphase cyctoplasmic foci protein 45
195115597 Dmoj\GI17333 284 Drosophila mojavensis eukaryota>metazoa>hexapoda GI17333 [Drosophila mojavensis].
194758495 Dana\GF14997 286 Drosophila ananassae eukaryota>metazoa>hexapoda GF14997 [Drosophila ananassae].
125987191 Dpse\GA17959 293 Drosophila pseudoobscura pseudoobscura eukaryota>metazoa>hexapoda GA17959 [Drosophila pseudoobscura pseudoobscura].
190579964 TRIADDRAFT_32525 245 Trichoplax adhaerens eukaryota>metazoa>placozoa hypothetical protein TRIADDRAFT_32525 [Trichoplax adhaerens].
196003010 TRIADDRAFT_55308 301 Trichoplax adhaerens eukaryota>metazoa>placozoa hypothetical protein TRIADDRAFT_55308 [Trichoplax adhaerens].
149412544 LOC100075219 269 Ornithorhynchus anatinus eukaryota>metazoa>vertebrata PREDICTED: hypothetical protein [Ornithorhynchus anatinus].
12853824 - 238 Mus musculus eukaryota>metazoa>vertebrata unnamed protein product [Mus musculus].
57085243 LOC479315 398 Canis lupus familiaris eukaryota>metazoa>vertebrata PREDICTED: similar to interphase cyctoplasmic foci protein 45
147904555 thg1l 286 Xenopus laevis eukaryota>metazoa>vertebrata tRNA-histidine guanylyltransferase 1-like [Xenopus laevis].
71895317 THG1L 269 Gallus gallus eukaryota>metazoa>vertebrata interphase cytoplasmic foci protein 45 [Gallus gallus].
126290678 LOC100025355 269 Monodelphis domestica eukaryota>metazoa>vertebrata PREDICTED: hypothetical protein [Monodelphis domestica].
12840654 - 150 Mus musculus eukaryota>metazoa>vertebrata unnamed protein product [Mus musculus].
224067593 LOC100223262 269 Taeniopygia guttata eukaryota>metazoa>vertebrata PREDICTED: interphase cytoplasmic foci protein 45 [Taeniopygia
109079563 LOC715853 298 Macaca mulatta eukaryota>metazoa>vertebrata PREDICTED: similar to interphase cyctoplasmic foci protein 45
114603135 LOC462226 298 Pan troglodytes eukaryota>metazoa>vertebrata PREDICTED: interphase cytoplasmic foci protein 45 [Pan
122692489 THG1L 298 Bos taurus eukaryota>metazoa>vertebrata probable tRNA(His) guanylyltransferase [Bos taurus].
124377988 Thg1l 298 Mus musculus eukaryota>metazoa>vertebrata probable tRNA(His) guanylyltransferase [Mus musculus].
149726693 LOC100071194 298 Equus caballus eukaryota>metazoa>vertebrata PREDICTED: similar to Probable tRNA(His) guanylyltransferase
281345114 PANDA_000301 298 Ailuropoda melanoleuca eukaryota>metazoa>vertebrata hypothetical protein PANDA_000301 [Ailuropoda melanoleuca].
291387726 LOC100353509 298 Oryctolagus cuniculus eukaryota>metazoa>vertebrata PREDICTED: CG4103-like [Oryctolagus cuniculus].
62078661 Thg1l 298 Rattus norvegicus eukaryota>metazoa>vertebrata probable tRNA(His) guanylyltransferase [Rattus norvegicus].
62530979 Thg1l 298 Mus musculus eukaryota>metazoa>vertebrata Thg1l protein [Mus musculus].
62897799 - 298 Homo sapiens eukaryota>metazoa>vertebrata interphase cyctoplasmic foci protein 45 variant [Homo sapiens].
26383091 - 279 Mus musculus eukaryota>metazoa>vertebrata unnamed protein product [Mus musculus].
7020726 - 298 Homo sapiens eukaryota>metazoa>vertebrata unnamed protein product [Homo sapiens].
89242148 THG1L 298 Homo sapiens eukaryota>metazoa>vertebrata probable tRNA(His) guanylyltransferase [Homo sapiens].
229366028 THG1 299 Anoplopoma fimbria eukaryota>metazoa>vertebrata>actinopterygii Probable tRNAHis guanylyltransferase [Anoplopoma fimbria].
47216813 GSTEN:00031660:G:001 277 Tetraodon nigroviridis eukaryota>metazoa>vertebrata>actinopterygii unnamed protein product [Tetraodon nigroviridis].
259089291 yg1g 299 Oncorhynchus mykiss eukaryota>metazoa>vertebrata>actinopterygii YGR024C [Oncorhynchus mykiss].
55925403 zgc:101609 269 Danio rerio eukaryota>metazoa>vertebrata>actinopterygii interphase cytoplasmic foci protein 45 [Danio rerio].
225716354 THG1 299 Esox lucius eukaryota>metazoa>vertebrata>actinopterygii Probable tRNAHis guanylyltransferase [Esox lucius].
213514008 thg1 299 Salmo salar eukaryota>metazoa>vertebrata>actinopterygii Probable tRNAHis guanylyltransferase [Salmo salar].
48309991 - 98 Arabidopsis thaliana eukaryota>viridiplantae At2g32320 [Arabidopsis thaliana].
3831464 At2g32320 190 Arabidopsis thaliana eukaryota>viridiplantae hypothetical protein [Arabidopsis thaliana].
3831463 At2g32330 297 Arabidopsis thaliana eukaryota>viridiplantae unknown protein [Arabidopsis thaliana].
168038223 PHYPADRAFT_217293 258 Physcomitrella patens subsp. patens eukaryota>viridiplantae predicted protein [Physcomitrella patens subsp. patens].
158283078 THG1 258 Chlamydomonas reinhardtii eukaryota>viridiplantae>chlorophyta histidine tRNA 5'-guanylyltransferase [Chlamydomonas reinhardtii].
123437995 TVAG_470220 237 Trichomonas vaginalis G3 eukaryota>parabasalia tRNAHis guanylyltransferase family protein [Trichomonas vaginalis
284097001 NAEGRDRAFT_61112 316 Naegleria gruberi eukaryota>heterolobosea predicted protein [Naegleria gruberi].
# 12; Thgi+Thgi
42569521 AT2G31580 567 Arabidopsis thaliana eukaryota>viridiplantae unknown protein [Arabidopsis thaliana].
238479416 AT2G32320 537 Arabidopsis thaliana eukaryota>viridiplantae unknown protein [Arabidopsis thaliana].
238479418 AT2G32320 532 Arabidopsis thaliana eukaryota>viridiplantae unknown protein [Arabidopsis thaliana].
147819926 VITISV_031884 530 Vitis vinifera eukaryota>viridiplantae hypothetical protein [Vitis vinifera].
225438521 LOC100264494 528 Vitis vinifera eukaryota>viridiplantae PREDICTED: hypothetical protein [Vitis vinifera].
186504734 AT2G32320 525 Arabidopsis thaliana eukaryota>viridiplantae unknown protein [Arabidopsis thaliana].
218197162 OsI_20769 524 Oryza sativa Indica Group eukaryota>viridiplantae hypothetical protein OsI_20769 [Oryza sativa Indica Group].
222632362 OsJ_19344 524 Oryza sativa Japonica Group eukaryota>viridiplantae hypothetical protein OsJ_19344 [Oryza sativa Japonica Group].
255578805 RCOM_0075620 524 Ricinus communis eukaryota>viridiplantae conserved hypothetical protein [Ricinus communis].
115465113 Os05g0535500 519 Oryza sativa Japonica Group eukaryota>viridiplantae Os05g0535500 [Oryza sativa (japonica cultivar-group)].
20198069 At2g31580 495 Arabidopsis thaliana eukaryota>viridiplantae unknown protein [Arabidopsis thaliana].
224065048 POPTRDRAFT_409100 482 Populus trichocarpa eukaryota>viridiplantae predicted protein [Populus trichocarpa].
45680430 OJ1014_C08.12 399 Oryza sativa Japonica Group eukaryota>viridiplantae putative tRNAHis guanylyltransferase [Oryza sativa Japonica Group].
# 1; false fusion; glycosyl hydrolase family 17+Thgi+Thgi
270232206 GSVIVT01022253001 1073 Vitis vinifera eukaryota>viridiplantae unnamed protein product [Vitis vinifera].
# 1; false fusion;PaaI_Thioesterase+Thgi
115492265 ATEG_00674 427 Aspergillus terreus NIH2624 eukaryota>fungi>ascomycota hypothetical protein ATEG_00674 [Aspergillus terreus NIH2624].
# 1; false fusion;Thgi+ST Protein Kinase (2jav)
281212173 thg1 731 Polysphondylium pallidum PN500 eukaryota>amoebozoa>mycetozoa>dictyosteliida tRNA-histidine guanylyltransferase 1 [Polysphondylium pallidum
# 1;
288922849 FrEUN1fDRAFT_6712 247 Frankia sp. EUN1f bacteria>actinobacteria hypothetical protein FrEUN1fDRAFT_6712 [Frankia sp. EUN1f].
163816590 COPEUT_02780 128 Coprococcus eutactus ATCC 27759 bacteria>firmicutes hypothetical protein COPEUT_02780 [Coprococcus eutactus ATCC
149908211 PE36_01857 288 Moritella sp. PE36 bacteria>proteobacteria>gammaproteobacteria hypothetical protein PE36_01857 [Moritella sp. PE36].
284085645 NAEGRDRAFT_72795 112 Naegleria gruberi eukaryota>heterolobosea predicted protein [Naegleria gruberi].
261194434 BDBG_06470 349 Ajellomyces dermatitidis SLH14081 eukaryota>fungi>ascomycota tRNA(His) guanylyltransferase [Ajellomyces dermatitidis SLH14081].
169622087 SNOG_14258 413 Phaeosphaeria nodorum SN15 eukaryota>fungi>ascomycota hypothetical protein SNOG_14258 [Phaeosphaeria nodorum SN15].
50552682 YALI0E09823g 309 Yarrowia lipolytica CLIB122 eukaryota>fungi>ascomycota YALI0E09823p [Yarrowia lipolytica].
71003728 UM00383.1 714 Ustilago maydis 521 eukaryota>fungi>basidiomycota hypothetical protein UM00383.1 [Ustilago maydis 521].
# 1;mispredicted as Cterminal only due to the pyrolysine being read as stop codon
20089700 MA0816 92 Methanosarcina acetivorans C2A archaea>euryarchaeota hypothetical protein MA0816 [Methanosarcina acetivorans C2A].
171913887 VspiD_010100021950 126 Verrucomicrobium spinosum DSM 4136 bacteria>chlamydiae/verrucomicrobia tRNAHis guanylyltransferase family protein [Verrucomicrobium
196258004 Cyan7822DRAFT_3698 61 Cyanothece sp. PCC 7822 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Cyanothece sp. PCC
196256338 Cyan7822DRAFT_2033 123 Cyanothece sp. PCC 7822 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Cyanothece sp. PCC
163816591 COPEUT_02781 112 Coprococcus eutactus ATCC 27759 bacteria>firmicutes hypothetical protein COPEUT_02781 [Coprococcus eutactus ATCC
295094970 CCU_28640 125 Coprococcus sp. ART55/1 bacteria>firmicutes Uncharacterized conserved protein [Coprococcus sp. ART55/1].
52548693 GZ1tured archaeon GZfos18C8 archaea uncharacterized conserved protein [uncultured archaeon GZfos18C8].
# 1;Fragment/Wrong start
149052344 LOC303067 128 Rattus norvegicus eukaryota>metazoa>vertebrata similar to hypothetical protein FLJ20546, isoform CRA_c [Rattus
270247845 GSVIVT01006301001 296 Vitis vinifera eukaryota>viridiplantae unnamed protein product [Vitis vinifera].
52549238 GZ26E7_19 212 uncultured archaeon GZfos26E7 archaea uncharacterized conserved protein [uncultured archaeon GZfos26E7].
196256820 Cyan7822DRAFT_2515 201 Cyanothece sp. PCC 7822 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Cyanothece sp. PCC
164659280 MGL_1763 225 Malassezia globosa CBS 7966 eukaryota>fungi>basidiomycota hypothetical protein MGL_1763 [Malassezia globosa CBS 7966].
259488914 ANIA_00804 235 Aspergillus nidulans FGSC A4 eukaryota>fungi>ascomycota TPA: tRNAHis guanylyltransferase Thg1, putative (AFU_orthologue;
110735837 At2g32330 205 Arabidopsis thaliana eukaryota>viridiplantae hypothetical protein [Arabidopsis thaliana].
67607657 Chro.10228 164 Cryptosporidium hominis TU502 eukaryota>alveolata>apicomplexa hypothetical protein [Cryptosporidium hominis TU502].
70928155 PC301576.00.0 221 Plasmodium chabaudi chabaudi eukaryota>alveolata>apicomplexa hypothetical protein [Plasmodium chabaudi chabaudi].
240108585 CPC735_009350 225 Coccidioides posadasii C735 delta SOWgp eukaryota>fungi>ascomycota tRNAHis guanylyltransferase family protein [Coccidioides posadasii
225561521 HCBG_01446 189 Ajellomyces capsulatus G186AR eukaryota>fungi>ascomycota tRNA guanylyltransferase [Ajellomyces capsulatus G186AR].
194386006 - 166 Homo sapiens eukaryota>metazoa>vertebrata unnamed protein product [Homo sapiens].
255606074 RCOM_1875060 185 Ricinus communis eukaryota>viridiplantae conserved hypothetical protein [Ricinus communis].
218197168 OsI_20778 122 Oryza sativa Indica Group eukaryota>viridiplantae hypothetical protein OsI_20778 [Oryza sativa Indica Group].
294464625 - 215 Picea sitchensis eukaryota>viridiplantae unknown [Picea sitchensis].
242088615 SORBIDRAFT_09g026710 152 Sorghum bicolor eukaryota>viridiplantae hypothetical protein SORBIDRAFT_09g026710 [Sorghum bicolor].
Top
Prokaryotic Thg1 domain containing proteins with operons
# 1;Thg1Top
124027917 Thg1*-> Hbut_1048 238 Hyperthermus butylicus DSM 5456 archaea>crenarchaeota hypothetical protein Hbut_1048 [Hyperthermus butylicus DSM 5456]. 124027914_?->124027915_?-><-124027916_?||124027917_Thg1*->124027918_?->124027919_?->124027920_?->
156937279 Thg1*-><-HTH Igni_0485 162 Ignicoccus hospitalis KIN4/I archaea>crenarchaeota hypothetical protein Igni_0485 [Ignicoccus hospitalis KIN4/I]. 156937276_?-><-156937277_?||156937278_?->156937279_Thg1*-><-156937280_HTH<-156937281_?||156937282_?->
18312250 AAA_ATPase->carboxypeptidase_Taq-><-Thg1*||CTP_transf_2_phosphopantetheine_adenylyltransferase-><-peptidase_M28 PAE0886 212 Pyrobaculum aerophilum str. IM2 archaea>crenarchaeota hypothetical protein PAE0886 [Pyrobaculum aerophilum str. IM2]. 18312247_?->18312248_AAA_ATPase->18312249_carboxypeptidase_Taq-><-18312250_Thg1*||18312251_CTP_transf_2_phosphopantetheine_adenylyltransferase-><-18312252_peptidase_M28<-18312253_?
145590370 AAA_ATPase->carboxypeptidase_Taq-><-Thg1*||CTP_transf_2_phosphopantetheine_adenylyltransferase-><-peptidase_M28 Pars_0105 196 Pyrobaculum arsenaticum DSM 13514 archaea>crenarchaeota hypothetical protein Pars_0105 [Pyrobaculum arsenaticum DSM 13514]. 145590367_?->145590368_AAA_ATPase->145590369_carboxypeptidase_Taq-><-145590370_Thg1*||145590371_CTP_transf_2_phosphopantetheine_adenylyltransferase-><-145590372_peptidase_M28<-145590373_?
126458698 AAA_ATPase->carboxypeptidase_Taq-><-Thg1*||CTP_transf_2_phosphopantetheine_adenylyltransferase-><-peptidase_M28 Pcal_0070 225 Pyrobaculum calidifontis JCM 11548 archaea>crenarchaeota hypothetical protein Pcal_0070 [Pyrobaculum calidifontis JCM 126458695_?->126458696_AAA_ATPase->126458697_carboxypeptidase_Taq-><-126458698_Thg1*||126458699_CTP_transf_2_phosphopantetheine_adenylyltransferase-><-126458700_peptidase_M28<-126458701_?
119872651 AAA_ATPase->carboxypeptidase_Taq-><-Thg1*||CTP_transf_2_phosphopantetheine_adenylyltransferase-><-peptidase_M28 Pisl_1144 212 Pyrobaculum islandicum DSM 4184 archaea>crenarchaeota hypothetical protein Pisl_1144 [Pyrobaculum islandicum DSM 4184]. 119872648_?->119872649_AAA_ATPase->119872650_carboxypeptidase_Taq-><-119872651_Thg1*||119872652_CTP_transf_2_phosphopantetheine_adenylyltransferase-><-119872653_peptidase_M28<-119872654_?
171184582 AAA_ATPase->carboxypeptidase_Taq-><-Thg1*||CTP_transf_2_phosphopantetheine_adenylyltransferase-><-peptidase_M28 Tneu_0097 212 Thermoproteus neutrophilus V24Sta archaea>crenarchaeota hypothetical protein Tneu_0097 [Thermoproteus neutrophilus V24Sta]. 171184579_?->171184580_AAA_ATPase->171184581_carboxypeptidase_Taq-><-171184582_Thg1*||171184583_CTP_transf_2_phosphopantetheine_adenylyltransferase-><-171184584_peptidase_M28<-171184585_?
154151809 isoleucyl-tRNA_synthetase-><-Thg1*<-PRC||S2P+CBS+CBS-><-RADICAL-SAM Mboo_2270 250 Candidatus Methanoregula boonei 6A8 archaea>euryarchaeota hypothetical protein Mboo_2270 [Candidatus Methanoregula boonei 154151806_?-><-154151807_?||154151808_isoleucyl-tRNA_synthetase-><-154151809_Thg1*<-154151810_PRC||154151811_S2P+CBS+CBS-><-154151812_RADICAL-SAM
126178352 RADICAL-SAM-><-S2P+CBS+CBS||PRC->Thg1*-><-isoleucyl-tRNA_synthetase||Lysine_decarbox-> Memar_0402 244 Methanoculleus marisnigri JR1 archaea>euryarchaeota hypothetical protein Memar_0402 [Methanoculleus marisnigri JR1]. 126178349_RADICAL-SAM-><-126178350_S2P+CBS+CBS||126178351_PRC->126178352_Thg1*-><-126178353_isoleucyl-tRNA_synthetase||126178354_Lysine_decarbox-><-126178355_?
219850835 RADICAL-SAM-><-S2P+CBS+CBS||PRC->Thg1*-><-isoleucyl-tRNA_synthetase||Lysine_decarbox-> Mpal_0151 243 Methanosphaerula palustris E1-9c archaea>euryarchaeota protein of unknown function DUF549 [Candidatus Methanosphaerula 219850832_RADICAL-SAM-><-219850833_S2P+CBS+CBS||219850834_PRC->219850835_Thg1*-><-219850836_isoleucyl-tRNA_synthetase||219850837_Lysine_decarbox-><-219850838_?
88601907 isoleucyl-tRNA_synthetase->??30S_ribosomal_protein_S8??-><-Thg1*<-PRC||?->S2P+CBS+CBS-> Mhun_0610 241 Methanospirillum hungatei JF-1 archaea>euryarchaeota hypothetical protein Mhun_0610 [Methanospirillum hungatei JF-1]. 88601904_?->88601905_isoleucyl-tRNA_synthetase->88601906_??30S_ribosomal_protein_S8??-><-88601907_Thg1*<-88601908_PRC||88601909_?->88601910_S2P+CBS+CBS->
82617278 <-glycyl-tRNA_synthetase<-4HB_Family_1_bi-partite_nucleotidyltransferase_subunit_DUF86<-Nucleotidyltransferase<-Thg1*<-PRC||S2P+CBS+CBS->THUMP+ThiI-> C4_0009 262 uncultured archaeon archaea conserved hypothetical protein [uncultured archaeon]. <-82617275_glycyl-tRNA_synthetase<-82617276_4HB_Family_1_bi-partite_nucleotidyltransferase_subunit_DUF86<-82617277_Nucleotidyltransferase<-82617278_Thg1*<-82617279_PRC||82617280_S2P+CBS+CBS->82617281_THUMP+ThiI->
52548693 <-glycyl-tRNA_synthetase<-4HB_Family_1_bi-partite_nucleotidyltransferase_subunit_DUF86<-Nucleotidyltransferase<-Thg1_Fragment*<-PRC||S2P+CBS+CBS->THUMP+ThiI-> GZ18C8_19 212 uncultured archaeon GZfos18C8 archaea uncharacterized conserved protein [uncultured archaeon GZfos18C8]. <-52548690_glycyl-tRNA_synthetase<-52548691_4HB_Family_1_bi-partite_nucleotidyltransferase_subunit_DUF86<-52548692_Nucleotidyltransferase<-52548693_Thg1_Fragment*<-52548694_PRC||52548695_S2P+CBS+CBS->52548696_THUMP+ThiI->
52549238 HTH-><-glycyl-tRNA_synthetase<-Nucleotidyltransferase<-Thg1_Fragment*<-PRC||S2P+CBS+CBS->THUMP+ThiI-> GZ26E7_19 212 uncultured archaeon GZfos26E7 archaea uncharacterized conserved protein [uncultured archaeon GZfos26E7]. 52549235_HTH-><-52549236_glycyl-tRNA_synthetase<-52549237_Nucleotidyltransferase<-52549238_Thg1_Fragment*<-52549239_PRC||52549240_S2P+CBS+CBS->52549241_THUMP+ThiI->
268323173 RNase_P-><-S2P+CBS+CBS||PRC->Thg1*-> BSM_02380 253 uncultured archaeon archaea conserved hypothetical protein containing tRNAHis 268323170_RNase_P-><-268323171_S2P+CBS+CBS||268323172_PRC->268323173_Thg1*->268323174_?-><-268323175_?||268323176_?->
284162827 <-Thg1*<-PRC||Ribosomal_L1->Ribosomal_L10-> Arcpr_1732 246 Archaeoglobus profundus DSM 5631 archaea>euryarchaeota protein of unknown function DUF549 [Archaeoglobus profundus DSM 284162824_?->284162825_?->284162826_?-><-284162827_Thg1*<-284162828_PRC||284162829_Ribosomal_L1->284162830_Ribosomal_L10->
288559933 EMAP->PRC->aspartate_dehydrogenase->Thg1*->JAB-><-2-Hacid_dh+ACT thgL 252 Methanobrevibacter ruminantium M1 archaea>euryarchaeota tRNA(His) guanylyltransferase ThgL [Methanobrevibacter ruminantium 288559930_EMAP->288559931_PRC->288559932_aspartate_dehydrogenase->288559933_Thg1*->288559934_JAB-><-288559935_2-Hacid_dh+ACT||288559936_?->
148642523 <-JAB<-Thg1*<-aspartate_dehydrogenase<-PRC||EMAP-> Msm_0463 239 Methanobrevibacter smithii ATCC 35061 archaea>euryarchaeota tRNA(His) guanylyltransferase [Methanobrevibacter smithii ATCC 148642520_?->148642521_?-><-148642522_JAB<-148642523_Thg1*<-148642524_aspartate_dehydrogenase<-148642525_PRC||148642526_EMAP->
261349483 <-JAB<-Thg1*<-aspartate_dehydrogenase<-PRC||EMAP-> METSMIF1_02117 239 Methanobrevibacter smithii DSM 2374 archaea>euryarchaeota tRNAHis guanylyltransferase family protein [Methanobrevibacter 261349480_?-><-261349481_?<-261349482_JAB<-261349483_Thg1*<-261349484_aspartate_dehydrogenase<-261349485_PRC||261349486_EMAP->
222445979 <-EMAP||PRC->aspartate_dehydrogenase->Thg1*->JAB-> METSMIALI_01627 239 Methanobrevibacter smithii DSM 2375 archaea>euryarchaeota hypothetical protein METSMIALI_01627 [Methanobrevibacter smithii <-222445976_EMAP||222445977_PRC->222445978_aspartate_dehydrogenase->222445979_Thg1*->222445980_JAB-><-222445981_?||222445982_?->
282165226 <-S2P+CBS+CBS||PRC-><-MFS_transporter||Thg1*-><-Maltose_acetyltransferase MCP_2556 247 Methanocella paludicola SANAE archaea>euryarchaeota hypothetical protein MCP_2556 [Methanocella paludicola SANAE]. <-282165223_S2P+CBS+CBS||282165224_PRC-><-282165225_MFS_transporter||282165226_Thg1*-><-282165227_Maltose_acetyltransferase<-282165228_?||282165229_?->
91773429 <-tyrosyl-tRNA_synthetase<-Thg1*<-PRC||DNA_PolI-> Mbur_1464 243 Methanococcoides burtonii DSM 6242 archaea>euryarchaeota hypothetical protein Mbur_1464 [Methanococcoides burtonii DSM <-91773426_?<-91773427_?<-91773428_tyrosyl-tRNA_synthetase<-91773429_Thg1*<-91773430_PRC||91773431_DNA_PolI->91773432_?->
294496410 <-tyrosyl-tRNA_synthetase<-Thg1*<-PRC||DNA_PolI-> Mmah_1763 243 Methanohalophilus mahii DSM 5219 archaea>euryarchaeota tRNA(His)-5'-guanylyltransferase [Methanohalophilus mahii DSM 294496407_?-><-294496408_?<-294496409_tyrosyl-tRNA_synthetase<-294496410_Thg1*<-294496411_PRC||294496412_DNA_PolI->294496413_?->
20089701 <-tyrosyl-tRNA_synthetase<-Thg1_C<-Thg1*<-PRC<-malate_dehydrogenase||DNA_PolI-> MA0817 141 Methanosarcina acetivorans C2A archaea>euryarchaeota hypothetical protein MA0817 [Methanosarcina acetivorans C2A]. <-20089698_?<-20089699_tyrosyl-tRNA_synthetase<-20089700_Thg1_C<-20089701_Thg1*<-20089702_PRC<-20089703_malate_dehydrogenase||20089704_DNA_PolI->
20089700 <-tyrosyl-tRNA_synthetase<-Thg1_C*<-Thg1<-PRC<-malate_dehydrogenase MA0816 92 Methanosarcina acetivorans C2A archaea>euryarchaeota hypothetical protein MA0816 [Methanosarcina acetivorans C2A]. <-20089697_?<-20089698_?<-20089699_tyrosyl-tRNA_synthetase<-20089700_Thg1_C*<-20089701_Thg1<-20089702_PRC<-20089703_malate_dehydrogenase
73669253 <-tyrosyl-tRNA_synthetase<-Thg1*<-PRC<-malate_dehydrogenase||DNA_PolI-> Mbar_A1746 243 Methanosarcina barkeri str. Fusaro archaea>euryarchaeota hypothetical protein Mbar_A1746 [Methanosarcina barkeri str. <-73669250_?<-73669251_?<-73669252_tyrosyl-tRNA_synthetase<-73669253_Thg1*<-73669254_PRC<-73669255_malate_dehydrogenase||73669256_DNA_PolI->
21228066 <-tyrosyl-tRNA_synthetase<-Thg1*<-PRC<-malate_dehydrogenase||DNA_PolI-> MM_1964 243 Methanosarcina mazei Go1 archaea>euryarchaeota hypothetical protein MM_1964 [Methanosarcina mazei Go1]. <-21228063_?<-21228064_?<-21228065_tyrosyl-tRNA_synthetase<-21228066_Thg1*<-21228067_PRC<-21228068_malate_dehydrogenase||21228069_DNA_PolI->
124485581 RADICAL-SAM-><-?||PRC->Thg1*->Thioredoxin->RNA-Helicase->TrmB_transcriptional_regulator-> Mlab_0759 240 Methanocorpusculum labreanum Z archaea>euryarchaeota hypothetical protein Mlab_0759 [Methanocorpusculum labreanum Z]. 124485578_RADICAL-SAM-><-124485579_?||124485580_PRC->124485581_Thg1*->124485582_Thioredoxin->124485583_RNA-Helicase->124485584_TrmB_transcriptional_regulator->
20093533 <-Thg1*<-aspartate_dehydrogenase<-RNA_Me_trans<-PRC MK0093 262 Methanopyrus kandleri AV19 archaea>euryarchaeota hypothetical protein MK0093 [Methanopyrus kandleri AV19]. 20093530_?->20093531_?-><-20093532_?<-20093533_Thg1*<-20093534_aspartate_dehydrogenase<-20093535_RNA_Me_trans<-20093536_PRC
116754185 <-Thg1*<-PRC Mthe_0875 235 Methanosaeta thermophila PT archaea>euryarchaeota hypothetical protein Mthe_0875 [Methanosaeta thermophila PT]. 116754182_?->116754183_?->116754184_?-><-116754185_Thg1*<-116754186_PRC||116754187_?->116754188_?->
84489939 2-Hacid_dh+ACT-><-JAB<-Thg1*<-aspartate_dehydrogenase<-PRC||EMAP-> Msp_1147 237 Methanosphaera stadtmanae DSM 3091 archaea>euryarchaeota hypothetical protein Msp_1147 [Methanosphaera stadtmanae DSM 3091]. <-84489936_?||84489937_2-Hacid_dh+ACT-><-84489938_JAB<-84489939_Thg1*<-84489940_aspartate_dehydrogenase<-84489941_PRC||84489942_EMAP->
15678990 2-Hacid_dh+ACT-><-JAB<-Thg1*<-aspartate_dehydrogenase<-PRC||EMAP-> MTH972 246 Methanothermobacter thermautotrophicus str. Delta H archaea>euryarchaeota hypothetical protein MTH972 [Methanothermobacter thermautotrophicus <-15678987_?||15678988_2-Hacid_dh+ACT-><-15678989_JAB<-15678990_Thg1*<-15678991_aspartate_dehydrogenase<-15678992_PRC||15678993_EMAP->
147920979 <-TPR<-Thg1*||MFS_transporter-><-PRC||S2P+CBS+CBS-> RCIX455 241 uncultured methanogenic archaeon RC-I archaea>euryarchaeota hypothetical protein RCIX455 [uncultured methanogenic archaeon 147920976_?->147920977_?-><-147920978_TPR<-147920979_Thg1*||147920980_MFS_transporter-><-147920981_PRC||147920982_S2P+CBS+CBS->
170290817 Thg1*-> Kcr_1204 249 Candidatus Korarchaeum cryptofilum OPF8 archaea>korarchaeota hypothetical protein Kcr_1204 [Candidatus Korarchaeum cryptofilum 170290814_?->170290815_?->170290816_?->170290817_Thg1*->170290818_?-><-170290819_?<-170290820_?
219882632 Thg1*-> Achl_4028 238 Arthrobacter chlorophenolicus A6 bacteria>actinobacteria hypothetical protein Achl_4028 [Arthrobacter chlorophenolicus A6]. <-219882629_?||219882630_?->219882631_?->219882632_Thg1*-><-219882633_?<-219882634_?<-219882635_?
252128517 <-Thg1* CORMA0001_0334 250 Corynebacterium matruchotii ATCC 14266 bacteria>actinobacteria trnahis guanylyltransferase family protein [Corynebacterium 252128514_?->252128515_?->252128516_?-><-252128517_Thg1*<-252128518_?||252128519_?->252128520_?->
225022489 Thg1*-> CORMATOL_02529 250 Corynebacterium matruchotii ATCC 33806 bacteria>actinobacteria hypothetical protein CORMATOL_02529 [Corynebacterium matruchotii 225022486_?->225022487_?->225022488_?->225022489_Thg1*-><-225022490_?||225022491_?->225022492_?->
54025916 <-Thg1* nfa39460 229 Nocardia farcinica IFM 10152 bacteria>actinobacteria hypothetical protein nfa39460 [Nocardia farcinica IFM 10152]. <-54025913_?||54025914_?-><-54025915_?<-54025916_Thg1*<-54025917_?<-54025918_?<-54025919_?
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254412407 <-Thg1* MC7420_6362 255 Microcoleus chthonoplastes PCC 7420 bacteria>cyanobacteria tRNAHis guanylyltransferase superfamily [Microcoleus chthonoplastes <-254412549_?||254412531_?-><-254412419_?<-254412407_Thg1*<-254412580_?||254412575_?->254412416_?->
196256337 Thg1*->Thg1_C-> Cyan7822DRAFT_2032 141 Cyanothece sp. PCC 7822 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Cyanothece sp. PCC 196256334_?-><-196256335_?||196256336_?->196256337_Thg1*->196256338_Thg1_C-><-196256339_?<-196256340_?
228982997 Thg1*-> bthur0002_61310 353 Bacillus thuringiensis Bt407 bacteria>firmicutes hypothetical protein bthur0002_61310 [Bacillus thuringiensis <-228982994_?||228982995_?-><-228982996_?||228982997_Thg1*->228982998_?->228982999_?->228983000_?->
75758411 <-Thg1* RBTH_06728 245 Bacillus thuringiensis serovar israelensis ATCC 35646 bacteria>firmicutes Hypothetical protein RBTH_06728 [Bacillus thuringiensis serovar 75758408_?-><-75758409_?<-75758410_?<-75758411_Thg1*<-75758412_?<-75758413_?<-75758414_?
291529569 <-Thg1* ERE_34100 285 Eubacterium rectale M104/1 bacteria>firmicutes Uncharacterized conserved protein [Eubacterium rectale M104/1]. <-291529566_?<-291529567_?<-291529568_?<-291529569_Thg1*<-291529570_?<-291529571_?<-291529572_?
168698293 <-Thg1* GobsU_010100002157 279 Gemmata obscuriglobus UQM 2246 bacteria>planctomycetes hypothetical protein GobsU_02157 [Gemmata obscuriglobus UQM 2246]. <-168698290_?<-168698291_?||168698292_?-><-168698293_Thg1*||168698294_?-><-168698295_?<-168698296_?
153872919 Thg1*-> BGP_4908 214 Beggiatoa sp. PS bacteria>proteobacteria>gammaproteobacteria protein of unknown function DUF549 [Beggiatoa sp. PS]. 153872919_Thg1*-><-153872920_?
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225075026 Thg1*->transcriptional_regulator_RtcR-> NEIFLAOT_00025 252 Neisseria flavescens NRL30031/H210 bacteria>proteobacteria>betaproteobacteria hypothetical protein NEIFLAOT_00025 [Neisseria flavescens 225075023_?->225075024_?-><-225075025_?||225075026_Thg1*->225075027_transcriptional_regulator_RtcR-><-225075028_?<-225075029_?
294671196 Thg1*->transcriptional_regulator_RtcR->Lysyl-tRNA_synthetase-> NEIELOOT_02904 255 Neisseria elongata subsp. glycolytica ATCC 29315 bacteria>proteobacteria>betaproteobacteria hypothetical protein NEIELOOT_02904 [Neisseria elongata subsp. 294671193_?-><-294671195_?||294671194_?->294671196_Thg1*->294671197_transcriptional_regulator_RtcR->294671198_Lysyl-tRNA_synthetase-><-294671199_?
255067726 <-transcriptional_regulator_RtcR<-Thg1* NEISICOT_02755 251 Neisseria sicca ATCC 29256 bacteria>proteobacteria>betaproteobacteria tRNA(His) guanylyltransferase family protein [Neisseria sicca ATCC 255067723_?-><-255067724_?<-255067725_transcriptional_regulator_RtcR<-255067726_Thg1*||255067727_?->255067728_?->255067729_?->
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258545199 <-transcriptional_regulator_RtcR<-false<-DNA_alkylation<-Thg1*<-Poly-Nucleotide--kinase HMPREF0198_1468 256 Cardiobacterium hominis ATCC 15826 bacteria>proteobacteria>gammaproteobacteria tRNAHis guanylyltransferase family protein [Cardiobacterium hominis <-258545196_transcriptional_regulator_RtcR<-258545197_false<-258545198_DNA_alkylation<-258545199_Thg1*<-258545200_Poly-Nucleotide--kinase||258545201_?->258545202_?->
256424765 Poly-Nucleotide--kinase->Thg1*-> Cpin_5797 255 Chitinophaga pinensis DSM 2588 bacteria>bacteroidetes/chlorobi tRNA(His)-5'-guanylyltransferase [Chitinophaga pinensis DSM 2588]. <-256424762_?||256424763_?->256424764_Poly-Nucleotide--kinase->256424765_Thg1*-><-256424766_?<-256424767_?||256424768_?->
124008957 <-Thg1*<-DinB_Hydrolase<-Poly-Nucleotide--kinase M23134_06676 250 Microscilla marina ATCC 23134 bacteria>bacteroidetes/chlorobi conserved protein [Microscilla marina ATCC 23134]. 124008954_?->124008955_?->124008956_?-><-124008957_Thg1*<-124008958_DinB_Hydrolase<-124008959_Poly-Nucleotide--kinase<-124008960_?
171913887 Poly-Nucleotide--kinase->Thg1->?->Thg1_C*-> VspiD_010100021950 126 Verrucomicrobium spinosum DSM 4136 bacteria>chlamydiae/verrucomicrobia tRNAHis guanylyltransferase family protein [Verrucomicrobium 171913884_Poly-Nucleotide--kinase->171913885_Thg1->171913886_?->171913887_Thg1_C*-><-171913888_?||171913889_?->171913890_?->
171913885 MACRODOMAIN->ADP-ribosylglycohydrolase->Poly-Nucleotide--kinase->Thg1*->?->Thg1_C-> VspiD_010100021940 159 Verrucomicrobium spinosum DSM 4136 bacteria>chlamydiae/verrucomicrobia tRNAHis guanylyltransferase family protein [Verrucomicrobium 171913882_MACRODOMAIN->171913883_ADP-ribosylglycohydrolase->171913884_Poly-Nucleotide--kinase->171913885_Thg1*->171913886_?->171913887_Thg1_C-><-171913888_?
159900562 <-Thg1*<-Poly-Nucleotide--kinase Haur_4049 252 Herpetosiphon aurantiacus ATCC 23779 bacteria>chloroflexi hypothetical protein Haur_4049 [Herpetosiphon aurantiacus ATCC 159900559_?->159900560_?-><-159900561_?<-159900562_Thg1*<-159900563_Poly-Nucleotide--kinase||159900564_?->159900565_?->
158336895 Poly-Nucleotide--kinase->Thg1*-> AM1_3767 253 Acaryochloris marina MBIC11017 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Acaryochloris marina 158336892_?->158336893_?->158336894_Poly-Nucleotide--kinase->158336895_Thg1*-><-158336896_?<-158336897_?<-158336898_?
269120695 Thg1*->Poly-Nucleotide--kinase-> Sterm_2087 241 Sebaldella termitidis ATCC 33386 bacteria>fusobacteria hypothetical protein Sterm_2087 [Sebaldella termitidis ATCC 33386]. 269120692_?->269120693_?->269120694_?->269120695_Thg1*->269120696_Poly-Nucleotide--kinase->269120697_?->269120698_?->
229539832 <-Thg1*<-Poly-Nucleotide--kinase PlimDRAFT_36970 258 Planctomyces limnophilus DSM 3776 bacteria>planctomycetes tRNA(His)-5'-guanylyltransferase [Planctomyces limnophilus DSM <-229539829_?<-229539830_?<-229539831_?<-229539832_Thg1*<-229539833_Poly-Nucleotide--kinase<-229539834_?||229539835_?->
162454339 Poly-Nucleotide--kinase->Thg1*-> sce6062 248 Sorangium cellulosum 'So ce 56' bacteria>proteobacteria>deltaproteobacteria hypothetical protein sce6062 [Sorangium cellulosum 'So ce 56']. 162454336_?->162454337_?->162454338_Poly-Nucleotide--kinase->162454339_Thg1*->162454340_?->162454341_?->162454342_?->
149920560 Poly-Nucleotide--kinase->Thg1*-> PPSIR1_23574 274 Plesiocystis pacifica SIR-1 bacteria>proteobacteria>deltaproteobacteria hypothetical protein PPSIR1_23574 [Plesiocystis pacifica SIR-1]. 149920557_?->149920558_?->149920559_Poly-Nucleotide--kinase->149920560_Thg1*-><-149920561_?||149920562_?->149920563_?->
108762077 <-Thg1*<-Poly-Nucleotide--kinase MXAN_5968 267 Myxococcus xanthus DK 1622 bacteria>proteobacteria>deltaproteobacteria tRNAHis guanylyltransferase family protein [Myxococcus xanthus DK 108756771_?->108758036_?->108761385_?-><-108762077_Thg1*<-108763390_Poly-Nucleotide--kinase||108762242_?-><-108761245_?
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163816591 RNA_ligase->Thg1->Thg1_C*->MACRODOMAIN-> COPEUT_02781 112 Coprococcus eutactus ATCC 27759 bacteria>firmicutes hypothetical protein COPEUT_02781 [Coprococcus eutactus ATCC 163816588_?->163816589_RNA_ligase->163816590_Thg1->163816591_Thg1_C*->163816592_MACRODOMAIN->163816593_?->163816594_?->
163816590 <-HD||?->RNA_ligase->Thg1*->Thg1_C->MACRODOMAIN-> COPEUT_02780 128 Coprococcus eutactus ATCC 27759 bacteria>firmicutes hypothetical protein COPEUT_02780 [Coprococcus eutactus ATCC <-163816587_HD||163816588_?->163816589_RNA_ligase->163816590_Thg1*->163816591_Thg1_C->163816592_MACRODOMAIN->163816593_?->
295094970 <-MACRODOMAIN<-Thg1_C* CCU_28640 125 Coprococcus sp. ART55/1 bacteria>firmicutes Uncharacterized conserved protein [Coprococcus sp. ART55/1]. <-295094967_?<-295094968_?<-295094969_MACRODOMAIN<-295094970_Thg1_C*<-295094971_?<-295094972_?<-295094973_?
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# 1; Thg1
288922849 Thg1*-> FrEUN1fDRAFT_6712 247 Frankia sp. EUN1f bacteria>actinobacteria hypothetical protein FrEUN1fDRAFT_6712 [Frankia sp. EUN1f]. 288922846_?->288922847_?->288922848_?->288922849_Thg1*-><-288922850_?||288922851_?->288922852_?->
149908211 <-S1COLD<-Thg1* PE36_01857 288 Moritella sp. PE36 bacteria>proteobacteria>gammaproteobacteria hypothetical protein PE36_01857 [Moritella sp. PE36]. <-149908208_?<-149908209_?<-149908210_S1COLD<-149908211_Thg1*<-149908212_?<-149908213_?<-149908214_?
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196258004 Thg1_C*-> Cyan7822DRAFT_3698 61 Cyanothece sp. PCC 7822 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Cyanothece sp. PCC 196258001_?->196258002_?->196258003_?->196258004_Thg1_C*-><-196258005_?<-196258006_?<-196258007_?
196256338 Thg1->Thg1_C*-> Cyan7822DRAFT_2033 123 Cyanothece sp. PCC 7822 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Cyanothece sp. PCC <-196256335_?||196256336_?->196256337_Thg1->196256338_Thg1_C*-><-196256339_?<-196256340_?||196256341_?->
# 1;Thg1_Frag
196256820 <-Thg1_Fragment*<-Thg1 Cyan7822DRAFT_2515 201 Cyanothece sp. PCC 7822 bacteria>cyanobacteria tRNAHis guanylyltransferase family protein [Cyanothece sp. PCC <-196256817_?||196256818_?->196256819_?-><-196256820_Thg1_Fragment*<-196256821_Thg1<-196256822_?||196256823_?->
Phylogenetic tree of Thg1 domain containing proteins
The tree was constructed with the Thg1 RRM-Palm domain alone (without the C terminal extension)
Archaea - Blue
Bacteria - Violet
Eukaryotes: Plants- Green; Animals- Red; Fungi- Grey; Apicomplexans- Brown; Amoebozoans/ bacterial transfers to eukaryotes- Ochre; Rest- Black
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