Figure 3.

Alignment of E. coli crotonase family members. Protein family membership was determined as proteins having sequence similarity of 200 Pam units or less over at least 50% of their length. Members of the E. coli crotonase family are listed in Table 3. The protein sequences were aligned with ClustalW 2.0.11. Identical residues are highlighted in dark grey while conserved and semi-conserved residues are highlighted in light grey. Residues forming the FadB oxanion hole used to stabilize reaction intermediates are shown in bold-face. The FadB reaction center is outlined.