Figure 2.

Predicted new families of toxins. A. Multiple alignment of COG4679 family (RelE interferase supefamily). B. Multiple alignment of SMa0917 family (PemK/MazF interferase superfamily). C. Multiple alignment of COG2929 family (RelE interferase superfamily) representative. The sequences are denoted by Gene Identification (GI) numbers from the GenBank database and abbreviated species names. Species name abbreviations (generally consisting of 3 first letters of genus name and 4 first letters of species) for all alignments are given in Additional file 13. The positions of the first and the last residues of the aligned region in the corresponding protein are indicated for each sequence. The numbers within the alignment represent poorly conserved inserts that are not shown. The coloring is based on the consensus shown underneath the alignment; h indicates hydrophobic residues (ACFILMVWY), p indicates polar residues (STEDKRNQH), s indicates small residues (AGSVC) and a indicates aromatic residues (WYFH). The secondary structure elements are shown according to structural data if the structure is available or predicted using the PSIPRED program [106]; E indicates β-strand and H indicates α-helix.