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Genomic analysis of an emerging multiresistant Staphylococcus aureus strain rapidly spreading in cystic fibrosis patients revealed the presence of an antibiotic inducible bacteriophage

Jean-Marc Rolain1*, Patrice François2, David Hernandez2, Fadi Bittar1, Hervé Richet1, Ghislain Fournous1, Yves Mattenberger2, Emmanuelle Bosdure3, Nathalie Stremler3, Jean-Christophe Dubus3, Jacques Sarles3, Martine Reynaud-Gaubert4, Stephanie Boniface4, Jacques Schrenzel2 and Didier Raoult1

Author Affiliations

1 URMITE CNRS-IRD UMR 6236, Faculté de Médecine et de Pharmacie, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France

2 Geneva University Hospitals and University of Geneva, Central Lab of Bacteriology, and Genomic Research Lab, 24 rue Micheli-du-Crest, CH-1211 Geneva 14, Switzerland

3 Département des Maladies Respiratoires, Centre de Ressources et de Compétences pour la Mucoviscidose Enfants (CRCM), Hôpital Timone, Marseille, France

4 Département des Maladies Respiratoires, Centre de Ressources et de Compétences pour la Mucoviscidose Adultes (CRCM), Hôpital Sainte Marguerite, Marseille, France

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Biology Direct 2009, 4:1 doi:10.1186/1745-6150-4-1

Published: 13 January 2009

Additional files

Additional file 1:

Figure S1. Profile analysis population with teicoplanin showing the h-GISA population.

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Additional file 2:

Table S1. List of genes down- and up-regulated in CF-Marseille as compared to other available genomes.

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Additional file 3:

Table S2. Patient information and typing of the strains. Study n°: number attributed to the strain in this study. R, resistant; S, sensitive. MecA: detection of the mecA gene. SCC Mec: type of the SCCmec cassette; SCND, cassette type not determined (i.e. not 1, 2 or 4). AGR: agr type. PVL, TSST-1, EXFO-A, EXFO-B: detection of the gene coding for the toxin.

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Additional file 4:

Figure S2. Complete Genome Hybridization results with other strains. Gene and strain clustering by GeneSpring (a). Dendogram based on the presence and absence of genes in the 12 strains on CGH results (b).

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