Discovery notes
A DNA topoisomerase IB in Thaumarchaeota testifies for the presence of this enzyme in the last common ancestor of Archaea and Eucarya
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* Corresponding author: Céline Brochier-Armanet celine.brochier@ibsm.cnrs-mrs.fr
1 Université de Provence, Aix-Marseille I, CNRS UPR9043, Laboratoire de Chimie Bactérienne, IFR88, Marseille, France
2 Institut Pasteur, 25 rue du Docteur roux, 75015 Paris, France
3 Univ Paris-sud, Institut de Génétique et Microbiologie, CNRS UMR8621, 91405 Orsay Cedex, France
Biology Direct 2008, 3:54 doi:10.1186/1745-6150-3-54
Published: 23 December 2008Additional files
Additional file 1:
Archaeal-topoin-af1. Multiple alignment of Topo IB sequences from three eukaryotes (Scerevisiae = Saccharomyces cerevisiae, Hsapiens = Homo sapiens, Osativa = Oryza sativa), the two thaumarchaeota (Cenarchaeum symbiosum and Nitrosopumilus maritimus), two bacteria (Oterrae = Opitutus terrae PB90-1 and Rlitoralis = Roseobacter litoralis Och 149) and three viruses (Apolyphaga = Acanthamoeba polyphaga mimivirus, Bpapular = Bovine papular stomatitis virus and Ymonkey = Yaba monkey tumor virus). Coloured boxes delineate the putative functional domains according to the PFAM database: Topoisom_I_N (PF02919, Eukaryotic DNA topoisomerase I, DNA binding fragment) in red, Topoisom_I (PF01028, Eukaryotic DNA topoisomerase I, catalytic core) in blue and virDNA-Topo-I_N (PF09266, Viral DNA topoisomerase I, N-terminal) in green. The N-ter regions of viral Topo IB share conserved residues with bacterial and mimiviral homologues, suggesting the presence of a virDNA-Topo-I_N-like domain in these sequences.
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Additional file 2:
Archaeal-topoin-af2. Material and methods.
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Additional file 3:
Archaeal-topoin-af3. Table showing the taxonomic distribution of the 95 Topo IB, 634 Topo IA sensu stricto, 369 Topo III and 40 Reverse gyrase sequences retrieved from the 670 complete bacterial and archaeal genomes available in June 2008.
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