Figure 1.

Schematic representation of the options available in the CAIcal server. Using a combination of three inputs (DNA or RNA sequences, a codon usage reference table and/or a protein alignment), the server calculates gene parameters such as %G+C, Relative Synonymous Codon Usage (RSCU) and Effective Number of Codons (ENc), the CAI for one or more DNA or RNA sequences and an expected CAI and represents the CAI along a DNA sequence or in a protein multialignment translated to DNA.