Table 1

Characterization of major cell types in the Idd9 islet infiltrate



Mean
Sem
Gate

Idd9
NOD
Idd9
NOD


%CD45
54.2
51.8
4.4
10.0

CD45+
%CD11b
30.8
26.3
3.2
6.2
CD45+
%CD11c
9.9
9.9
1.2
2.9
CD45+
%B220
42.7
36.5
4.0
6.5
CD45+
%CD4
14.0
16.9
1.3
1.0
CD45+
%CD8
1.5
6.8
0.6
1.7

Islets isolated from the pancreas of 12 week old female Idd9 congenic (n = 5) and NOD (n = 5) mice using histopaque density gradient and stained for analysis by flow cytometry to identify the major infiltrating leukocyte populations. Staining for CD45 was used to gate on the leukocyte population. Representative of two independent experiments.

Hill et al. Biology Direct 2007 2:5   doi:10.1186/1745-6150-2-5

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