Figure 5.

Evolution of the peroxisomal proteome. Biochemical pathways reconstructed according to KEGG and annotations of peroxisomal proteins. For details on the reconstruction of ancestral states see supplemental material. Color code: yellow, eukaryotic origin; green, alpha-proteobacterial origin; red, actinomycetales origin; blue, cyanobacterial origin; white, origin unresolved. Note that the ancestral eukaryotic peroxisomal proteome reconstruction depends on the topology of the eukaryotic tree. If an alternative topology is considered, placing kinetoplastida and viridiplantae together [49], and the plant peroxisomal proteome is taken from the Araperox database [31], then the reconstructed ancestral eukaryotic peroxisomal proteome would be much larger, including all proteins present in the opisthokont proteome except for ANT1, IDP3, FOX3, PEX13 and PEX19.