Figure 2.

Immunofluorescence staining of tight junction proteins. (A) Protocol for visualization of tight junction proteins in monolayer of cells grown on filter support (see Methods for details). (B) Confocal images of T84 cells scanned at 1 μm intervals. Occludin (green) and PKC zeta (red) are localized to the apical tight junction domain. Merged images show both proteins appearing and disappearing together in the same confocal planes.