Research
Proteomic and bioinformatic analysis of epithelial tight junction reveals an unexpected cluster of synaptic molecules
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Correspondence: Vivian W Tang vivian_tang@hms.harvard.edu
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Biology Direct 2006, 1:37 doi:10.1186/1745-6150-1-37
Published: 8 December 2006Additional files
Additional File 1:
Identification and scoring of hits. (A) Bands from 3 separate purifications were used for mass spectroscopy (see Methods for details). (B) Peptides from the same bands were pooled for fingerprint analysis.
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Additional File 2:
Filemaker prototype for bioinformatic analysis
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Additional File 3:
Co-enrichment of hits with occludin. (A) Western blots of whole cell, WC, and heavy plasma membrane, HPM, showing co-enrichment of ERK3, STAT2, Homer, Connexin 36, and mGluR5 with occludin. EAAT1 and Hsc70 are also present in the heavy plasma fraction (see Methods for details). (B) Antibodies used in this study.
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